Experiment: NAG (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0561 and Shew_0562 are separated by 100 nucleotides Shew_0562 and Shew_0563 overlap by 14 nucleotides Shew_0563 and Shew_0564 are separated by 9 nucleotides
Shew_0561: Shew_0561 - carbohydrate kinase, YjeF-related protein (RefSeq), at 635,897 to 637,375
_0561
Shew_0562: Shew_0562 - hypothetical protein (RefSeq), at 637,476 to 637,949
_0562
Shew_0563: Shew_0563 - N-acetylmuramoyl-L-alanine amidase (RefSeq), at 637,936 to 639,255
_0563
Shew_0564: Shew_0564 - DNA mismatch repair protein MutL (RefSeq), at 639,265 to 641,160
_0564
Position (kb) 637
638
639
640 Strain fitness (log2 ratio) -1
0
1 at 637.105 kb on + strand, within Shew_0561 at 637.369 kb on + strand at 637.369 kb on + strand at 637.377 kb on - strand at 637.377 kb on - strand at 638.948 kb on + strand, within Shew_0563 at 639.209 kb on - strand at 639.355 kb on + strand at 639.363 kb on - strand at 639.363 kb on - strand at 639.478 kb on - strand, within Shew_0564 at 639.494 kb on + strand, within Shew_0564 at 639.494 kb on + strand, within Shew_0564 at 639.494 kb on + strand, within Shew_0564 at 639.613 kb on - strand, within Shew_0564 at 639.638 kb on - strand, within Shew_0564 at 639.652 kb on - strand, within Shew_0564 at 639.962 kb on + strand, within Shew_0564 at 640.178 kb on + strand, within Shew_0564 at 640.186 kb on - strand, within Shew_0564 at 640.225 kb on - strand, within Shew_0564
Per-strain Table
Position Strand Gene LocusTag Fraction NAG (C) remove 637,105 + Shew_0561 0.82 +0.1 637,369 + +1.2 637,369 + +0.5 637,377 - +0.4 637,377 - +0.5 638,948 + Shew_0563 0.77 -0.6 639,209 - -0.5 639,355 + -0.5 639,363 - -1.1 639,363 - -0.5 639,478 - Shew_0564 0.11 -1.0 639,494 + Shew_0564 0.12 -0.6 639,494 + Shew_0564 0.12 -0.2 639,494 + Shew_0564 0.12 -0.6 639,613 - Shew_0564 0.18 -0.3 639,638 - Shew_0564 0.20 -0.2 639,652 - Shew_0564 0.20 -0.8 639,962 + Shew_0564 0.37 -0.4 640,178 + Shew_0564 0.48 +0.8 640,186 - Shew_0564 0.49 -0.8 640,225 - Shew_0564 0.51 -0.8
Or see this region's nucleotide sequence