Experiment: NAG (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0061 and Shew_0062 are separated by 491 nucleotides Shew_0062 and Shew_0063 are separated by 17 nucleotides Shew_0063 and Shew_0064 are separated by 20 nucleotides Shew_0064 and Shew_0065 are separated by 68 nucleotides
Shew_0061: Shew_0061 - formate dehydrogenase, gamma subunit (RefSeq), at 73,390 to 74,397
_0061
Shew_0062: Shew_0062 - hypothetical protein (RefSeq), at 74,889 to 75,086
_0062
Shew_0063: Shew_0063 - molybdopterin oxidoreductase (RefSeq), at 75,104 to 77,959
_0063
Shew_0064: Shew_0064 - 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein (RefSeq), at 77,980 to 78,549
_0064
Shew_0065: Shew_0065 - formate dehydrogenase, gamma subunit (RefSeq), at 78,618 to 79,610
_0065
Position (kb)
75
76
77
78 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 74.119 kb on - strand, within Shew_0061 at 74.119 kb on - strand, within Shew_0061 at 74.130 kb on + strand, within Shew_0061 at 74.295 kb on - strand, within Shew_0061 at 74.330 kb on - strand at 74.330 kb on - strand at 74.332 kb on + strand at 74.332 kb on + strand at 74.332 kb on + strand at 74.340 kb on - strand at 74.340 kb on - strand at 74.399 kb on - strand at 74.642 kb on + strand at 74.653 kb on + strand at 74.653 kb on + strand at 74.661 kb on - strand at 74.661 kb on - strand at 75.419 kb on - strand, within Shew_0063 at 75.712 kb on + strand, within Shew_0063 at 75.718 kb on + strand, within Shew_0063 at 75.726 kb on - strand, within Shew_0063 at 75.726 kb on - strand, within Shew_0063 at 75.897 kb on + strand, within Shew_0063 at 75.905 kb on - strand, within Shew_0063 at 75.909 kb on + strand, within Shew_0063 at 75.910 kb on - strand, within Shew_0063 at 76.063 kb on - strand, within Shew_0063 at 76.072 kb on + strand, within Shew_0063 at 76.086 kb on + strand, within Shew_0063 at 76.488 kb on + strand, within Shew_0063 at 76.574 kb on + strand, within Shew_0063 at 76.719 kb on - strand, within Shew_0063 at 76.719 kb on - strand, within Shew_0063 at 76.932 kb on + strand, within Shew_0063 at 76.994 kb on + strand, within Shew_0063 at 77.047 kb on + strand, within Shew_0063 at 77.060 kb on - strand, within Shew_0063 at 77.060 kb on - strand, within Shew_0063 at 77.060 kb on - strand, within Shew_0063 at 77.083 kb on - strand, within Shew_0063 at 77.156 kb on + strand, within Shew_0063 at 77.164 kb on - strand, within Shew_0063 at 77.256 kb on - strand, within Shew_0063 at 77.370 kb on + strand, within Shew_0063 at 77.608 kb on + strand, within Shew_0063 at 77.621 kb on - strand, within Shew_0063 at 77.751 kb on - strand at 77.794 kb on - strand at 77.882 kb on + strand at 77.928 kb on + strand at 78.067 kb on + strand, within Shew_0064 at 78.075 kb on - strand, within Shew_0064 at 78.138 kb on - strand, within Shew_0064 at 78.265 kb on + strand, within Shew_0064 at 78.265 kb on + strand, within Shew_0064 at 78.372 kb on - strand, within Shew_0064 at 78.519 kb on - strand at 78.568 kb on - strand at 78.579 kb on - strand at 78.712 kb on + strand at 78.817 kb on + strand, within Shew_0065 at 78.853 kb on + strand, within Shew_0065
Per-strain Table
Position Strand Gene LocusTag Fraction NAG (C) remove 74,119 - Shew_0061 0.72 +0.5 74,119 - Shew_0061 0.72 +0.2 74,130 + Shew_0061 0.73 -1.3 74,295 - Shew_0061 0.90 -0.7 74,330 - +1.3 74,330 - -3.1 74,332 + -0.1 74,332 + -1.3 74,332 + -0.7 74,340 - +0.1 74,340 - -0.5 74,399 - +1.1 74,642 + +0.1 74,653 + +1.2 74,653 + +0.2 74,661 - -0.1 74,661 - +0.1 75,419 - Shew_0063 0.11 +0.3 75,712 + Shew_0063 0.21 -1.3 75,718 + Shew_0063 0.21 +0.2 75,726 - Shew_0063 0.22 +0.5 75,726 - Shew_0063 0.22 +0.3 75,897 + Shew_0063 0.28 -0.8 75,905 - Shew_0063 0.28 -0.3 75,909 + Shew_0063 0.28 -0.8 75,910 - Shew_0063 0.28 -0.6 76,063 - Shew_0063 0.34 +0.0 76,072 + Shew_0063 0.34 -0.2 76,086 + Shew_0063 0.34 +0.3 76,488 + Shew_0063 0.48 -1.0 76,574 + Shew_0063 0.51 -0.3 76,719 - Shew_0063 0.57 +0.2 76,719 - Shew_0063 0.57 -0.4 76,932 + Shew_0063 0.64 +0.6 76,994 + Shew_0063 0.66 +0.7 77,047 + Shew_0063 0.68 +0.1 77,060 - Shew_0063 0.68 -0.3 77,060 - Shew_0063 0.68 -0.4 77,060 - Shew_0063 0.68 -0.9 77,083 - Shew_0063 0.69 -0.0 77,156 + Shew_0063 0.72 +0.7 77,164 - Shew_0063 0.72 +0.1 77,256 - Shew_0063 0.75 -0.1 77,370 + Shew_0063 0.79 -2.0 77,608 + Shew_0063 0.88 +0.7 77,621 - Shew_0063 0.88 +0.2 77,751 - +0.2 77,794 - -0.0 77,882 + -0.5 77,928 + +0.4 78,067 + Shew_0064 0.15 +0.1 78,075 - Shew_0064 0.17 +0.3 78,138 - Shew_0064 0.28 +0.5 78,265 + Shew_0064 0.50 -0.7 78,265 + Shew_0064 0.50 +0.1 78,372 - Shew_0064 0.69 +0.2 78,519 - -0.7 78,568 - +0.3 78,579 - +0.2 78,712 + -0.3 78,817 + Shew_0065 0.20 +1.3 78,853 + Shew_0065 0.24 +0.3
Or see this region's nucleotide sequence