Experiment: R2A pH7
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS10345 and LRK54_RS10350 are separated by 111 nucleotides LRK54_RS10350 and LRK54_RS10355 are separated by 119 nucleotides LRK54_RS10355 and LRK54_RS10360 are separated by 120 nucleotides
LRK54_RS10345: LRK54_RS10345 - 2-isopropylmalate synthase, at 2,279,618 to 2,281,273
_RS10345
LRK54_RS10350: LRK54_RS10350 - hypothetical protein, at 2,281,385 to 2,281,522
_RS10350
LRK54_RS10355: LRK54_RS10355 - threonine synthase, at 2,281,642 to 2,282,952
_RS10355
LRK54_RS10360: LRK54_RS10360 - homoserine kinase, at 2,283,073 to 2,284,068
_RS10360
Position (kb)
2281
2282
2283 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2280.669 kb on + strand, within LRK54_RS10345 at 2280.670 kb on - strand, within LRK54_RS10345 at 2280.670 kb on - strand, within LRK54_RS10345 at 2280.670 kb on - strand, within LRK54_RS10345 at 2280.705 kb on + strand, within LRK54_RS10345 at 2280.705 kb on + strand, within LRK54_RS10345 at 2280.706 kb on - strand, within LRK54_RS10345 at 2280.706 kb on - strand, within LRK54_RS10345 at 2280.790 kb on + strand, within LRK54_RS10345 at 2280.790 kb on + strand, within LRK54_RS10345 at 2280.962 kb on + strand, within LRK54_RS10345 at 2281.226 kb on + strand at 2281.226 kb on + strand at 2281.226 kb on + strand at 2281.226 kb on + strand at 2281.226 kb on + strand at 2281.227 kb on - strand at 2281.227 kb on - strand at 2281.227 kb on - strand at 2281.364 kb on + strand at 2281.364 kb on + strand at 2281.364 kb on + strand at 2281.364 kb on + strand at 2281.364 kb on + strand at 2281.364 kb on + strand at 2281.365 kb on - strand at 2281.365 kb on - strand at 2281.365 kb on - strand at 2281.365 kb on - strand at 2281.365 kb on - strand at 2281.365 kb on - strand at 2281.365 kb on - strand at 2281.365 kb on - strand at 2281.365 kb on - strand at 2281.368 kb on + strand at 2281.368 kb on + strand at 2281.369 kb on - strand at 2281.369 kb on - strand at 2281.369 kb on - strand at 2281.369 kb on - strand at 2281.369 kb on - strand at 2281.405 kb on + strand, within LRK54_RS10350 at 2281.405 kb on + strand, within LRK54_RS10350 at 2281.406 kb on - strand, within LRK54_RS10350 at 2281.406 kb on - strand, within LRK54_RS10350 at 2281.406 kb on - strand, within LRK54_RS10350 at 2281.408 kb on + strand, within LRK54_RS10350 at 2281.408 kb on + strand, within LRK54_RS10350 at 2281.408 kb on + strand, within LRK54_RS10350 at 2281.409 kb on - strand, within LRK54_RS10350 at 2281.409 kb on - strand, within LRK54_RS10350 at 2281.409 kb on - strand, within LRK54_RS10350 at 2281.409 kb on - strand, within LRK54_RS10350 at 2281.409 kb on - strand, within LRK54_RS10350 at 2281.409 kb on - strand, within LRK54_RS10350 at 2281.409 kb on - strand, within LRK54_RS10350 at 2281.409 kb on - strand, within LRK54_RS10350 at 2281.415 kb on + strand, within LRK54_RS10350 at 2281.415 kb on + strand, within LRK54_RS10350 at 2281.421 kb on + strand, within LRK54_RS10350 at 2281.422 kb on - strand, within LRK54_RS10350 at 2281.422 kb on - strand, within LRK54_RS10350 at 2281.429 kb on + strand, within LRK54_RS10350 at 2281.429 kb on + strand, within LRK54_RS10350 at 2281.429 kb on + strand, within LRK54_RS10350 at 2281.429 kb on + strand, within LRK54_RS10350 at 2281.429 kb on + strand, within LRK54_RS10350 at 2281.432 kb on + strand, within LRK54_RS10350 at 2281.432 kb on + strand, within LRK54_RS10350 at 2281.432 kb on + strand, within LRK54_RS10350 at 2281.432 kb on + strand, within LRK54_RS10350 at 2281.432 kb on + strand, within LRK54_RS10350 at 2281.432 kb on + strand, within LRK54_RS10350 at 2281.432 kb on + strand, within LRK54_RS10350 at 2281.432 kb on + strand, within LRK54_RS10350 at 2281.432 kb on + strand, within LRK54_RS10350 at 2281.432 kb on + strand, within LRK54_RS10350 at 2281.432 kb on + strand, within LRK54_RS10350 at 2281.433 kb on - strand, within LRK54_RS10350 at 2281.433 kb on - strand, within LRK54_RS10350 at 2281.534 kb on + strand at 2281.638 kb on + strand at 2281.638 kb on + strand at 2281.638 kb on + strand at 2281.638 kb on + strand at 2281.639 kb on - strand at 2281.639 kb on - strand at 2281.639 kb on - strand at 2282.072 kb on + strand, within LRK54_RS10355 at 2282.258 kb on + strand, within LRK54_RS10355 at 2282.259 kb on - strand, within LRK54_RS10355 at 2282.259 kb on - strand, within LRK54_RS10355 at 2282.261 kb on + strand, within LRK54_RS10355 at 2282.261 kb on + strand, within LRK54_RS10355 at 2282.261 kb on + strand, within LRK54_RS10355 at 2282.261 kb on + strand, within LRK54_RS10355 at 2282.261 kb on + strand, within LRK54_RS10355 at 2282.261 kb on + strand, within LRK54_RS10355 at 2282.262 kb on - strand, within LRK54_RS10355 at 2282.262 kb on - strand, within LRK54_RS10355 at 2282.262 kb on - strand, within LRK54_RS10355 at 2282.262 kb on - strand, within LRK54_RS10355 at 2282.852 kb on + strand at 2282.852 kb on + strand at 2282.852 kb on + strand at 2282.852 kb on + strand at 2282.852 kb on + strand at 2282.852 kb on + strand at 2282.853 kb on - strand at 2282.853 kb on - strand at 2282.853 kb on - strand at 2282.853 kb on - strand at 2282.853 kb on - strand at 2282.853 kb on - strand at 2282.853 kb on - strand at 2282.853 kb on - strand at 2282.933 kb on + strand at 2283.218 kb on + strand, within LRK54_RS10360 at 2283.218 kb on + strand, within LRK54_RS10360 at 2283.218 kb on + strand, within LRK54_RS10360 at 2283.218 kb on + strand, within LRK54_RS10360 at 2283.219 kb on - strand, within LRK54_RS10360 at 2283.219 kb on - strand, within LRK54_RS10360 at 2283.219 kb on - strand, within LRK54_RS10360 at 2283.219 kb on - strand, within LRK54_RS10360 at 2283.444 kb on - strand, within LRK54_RS10360 at 2283.444 kb on - strand, within LRK54_RS10360 at 2283.647 kb on + strand, within LRK54_RS10360 at 2283.647 kb on + strand, within LRK54_RS10360 at 2283.648 kb on - strand, within LRK54_RS10360 at 2283.648 kb on - strand, within LRK54_RS10360
Per-strain Table
Position Strand Gene LocusTag Fraction R2A pH7 remove 2,280,669 + LRK54_RS10345 0.63 +0.3 2,280,670 - LRK54_RS10345 0.64 +0.0 2,280,670 - LRK54_RS10345 0.64 +0.1 2,280,670 - LRK54_RS10345 0.64 +1.6 2,280,705 + LRK54_RS10345 0.66 +0.3 2,280,705 + LRK54_RS10345 0.66 -0.7 2,280,706 - LRK54_RS10345 0.66 -0.5 2,280,706 - LRK54_RS10345 0.66 +0.4 2,280,790 + LRK54_RS10345 0.71 +0.2 2,280,790 + LRK54_RS10345 0.71 -1.7 2,280,962 + LRK54_RS10345 0.81 -0.6 2,281,226 + +0.2 2,281,226 + -0.4 2,281,226 + -1.5 2,281,226 + -0.5 2,281,226 + -0.5 2,281,227 - -0.9 2,281,227 - +0.4 2,281,227 - -1.4 2,281,364 + -1.7 2,281,364 + -0.4 2,281,364 + +0.8 2,281,364 + -0.6 2,281,364 + -0.9 2,281,364 + -0.3 2,281,365 - +0.6 2,281,365 - +3.3 2,281,365 - +0.0 2,281,365 - +0.6 2,281,365 - +0.7 2,281,365 - -0.2 2,281,365 - +0.0 2,281,365 - +1.6 2,281,365 - -0.3 2,281,368 + -1.5 2,281,368 + -1.8 2,281,369 - -0.3 2,281,369 - +0.1 2,281,369 - +0.1 2,281,369 - +1.6 2,281,369 - -0.6 2,281,405 + LRK54_RS10350 0.14 -0.1 2,281,405 + LRK54_RS10350 0.14 +1.8 2,281,406 - LRK54_RS10350 0.15 -0.1 2,281,406 - LRK54_RS10350 0.15 -0.4 2,281,406 - LRK54_RS10350 0.15 +3.6 2,281,408 + LRK54_RS10350 0.17 -1.4 2,281,408 + LRK54_RS10350 0.17 -0.2 2,281,408 + LRK54_RS10350 0.17 -0.8 2,281,409 - LRK54_RS10350 0.17 -0.1 2,281,409 - LRK54_RS10350 0.17 -0.2 2,281,409 - LRK54_RS10350 0.17 -0.3 2,281,409 - LRK54_RS10350 0.17 +1.0 2,281,409 - LRK54_RS10350 0.17 +0.3 2,281,409 - LRK54_RS10350 0.17 +0.5 2,281,409 - LRK54_RS10350 0.17 +0.8 2,281,409 - LRK54_RS10350 0.17 +0.2 2,281,415 + LRK54_RS10350 0.22 -0.1 2,281,415 + LRK54_RS10350 0.22 -1.5 2,281,421 + LRK54_RS10350 0.26 -0.0 2,281,422 - LRK54_RS10350 0.27 +0.1 2,281,422 - LRK54_RS10350 0.27 -0.2 2,281,429 + LRK54_RS10350 0.32 -1.1 2,281,429 + LRK54_RS10350 0.32 -0.7 2,281,429 + LRK54_RS10350 0.32 -0.4 2,281,429 + LRK54_RS10350 0.32 -0.7 2,281,429 + LRK54_RS10350 0.32 -0.6 2,281,432 + LRK54_RS10350 0.34 -0.7 2,281,432 + LRK54_RS10350 0.34 -0.7 2,281,432 + LRK54_RS10350 0.34 -0.6 2,281,432 + LRK54_RS10350 0.34 +1.0 2,281,432 + LRK54_RS10350 0.34 -0.6 2,281,432 + LRK54_RS10350 0.34 -0.4 2,281,432 + LRK54_RS10350 0.34 -1.0 2,281,432 + LRK54_RS10350 0.34 +0.6 2,281,432 + LRK54_RS10350 0.34 -0.4 2,281,432 + LRK54_RS10350 0.34 -0.6 2,281,432 + LRK54_RS10350 0.34 -0.7 2,281,433 - LRK54_RS10350 0.35 -0.7 2,281,433 - LRK54_RS10350 0.35 -0.2 2,281,534 + +0.0 2,281,638 + -0.4 2,281,638 + +1.5 2,281,638 + +0.1 2,281,638 + +1.8 2,281,639 - -0.1 2,281,639 - +0.6 2,281,639 - -0.1 2,282,072 + LRK54_RS10355 0.33 -1.2 2,282,258 + LRK54_RS10355 0.47 -0.9 2,282,259 - LRK54_RS10355 0.47 -0.2 2,282,259 - LRK54_RS10355 0.47 -0.2 2,282,261 + LRK54_RS10355 0.47 +2.6 2,282,261 + LRK54_RS10355 0.47 -1.2 2,282,261 + LRK54_RS10355 0.47 +1.4 2,282,261 + LRK54_RS10355 0.47 -1.6 2,282,261 + LRK54_RS10355 0.47 +0.6 2,282,261 + LRK54_RS10355 0.47 +0.0 2,282,262 - LRK54_RS10355 0.47 -0.9 2,282,262 - LRK54_RS10355 0.47 +0.0 2,282,262 - LRK54_RS10355 0.47 -0.6 2,282,262 - LRK54_RS10355 0.47 -0.4 2,282,852 + -1.4 2,282,852 + -1.8 2,282,852 + +1.0 2,282,852 + -3.1 2,282,852 + -0.6 2,282,852 + -1.7 2,282,853 - +0.4 2,282,853 - -0.9 2,282,853 - +0.0 2,282,853 - +0.0 2,282,853 - +0.6 2,282,853 - +0.6 2,282,853 - +0.0 2,282,853 - -0.6 2,282,933 + -1.0 2,283,218 + LRK54_RS10360 0.15 +0.0 2,283,218 + LRK54_RS10360 0.15 -0.2 2,283,218 + LRK54_RS10360 0.15 -1.2 2,283,218 + LRK54_RS10360 0.15 -1.7 2,283,219 - LRK54_RS10360 0.15 -0.4 2,283,219 - LRK54_RS10360 0.15 -1.2 2,283,219 - LRK54_RS10360 0.15 -0.6 2,283,219 - LRK54_RS10360 0.15 +0.2 2,283,444 - LRK54_RS10360 0.37 +0.3 2,283,444 - LRK54_RS10360 0.37 -0.5 2,283,647 + LRK54_RS10360 0.58 -0.8 2,283,647 + LRK54_RS10360 0.58 +1.0 2,283,648 - LRK54_RS10360 0.58 +0.4 2,283,648 - LRK54_RS10360 0.58 +1.6
Or see this region's nucleotide sequence