Experiment: 6 days starvation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2303 and DvMF_2304 are separated by 161 nucleotides DvMF_2304 and hisH overlap by 8 nucleotides hisH and DvMF_2306 are separated by 712 nucleotides
DvMF_2303: DvMF_2303 - esterase/lipase-like protein (RefSeq), at 2,893,323 to 2,894,333
_2303
DvMF_2304: DvMF_2304 - imidazole glycerol phosphate synthase subunit HisF (RefSeq), at 2,894,495 to 2,895,274
_2304
DvMF_2305: hisH - IGP synthase, amidotransferase subunit (EC 4.3.2.10) (from data) , at 2,895,267 to 2,895,905
hisH
DvMF_2306: DvMF_2306 - carbamoyl-phosphate synthase, large subunit (RefSeq), at 2,896,618 to 2,899,854
_2306
Position (kb)
2895
2896 Strain fitness (log2 ratio)
-8
-7
-6
-5
-4
-3
-2
-1
0
1 at 2894.512 kb on + strand at 2894.553 kb on + strand at 2894.615 kb on - strand, within DvMF_2304 at 2894.896 kb on + strand, within DvMF_2304 at 2895.003 kb on + strand, within DvMF_2304 at 2895.005 kb on - strand, within DvMF_2304 at 2895.216 kb on + strand at 2895.282 kb on - strand at 2895.411 kb on + strand, within hisH at 2895.738 kb on - strand, within hisH at 2895.840 kb on - strand, within hisH at 2895.896 kb on - strand at 2895.958 kb on + strand at 2896.166 kb on + strand at 2896.658 kb on + strand at 2896.750 kb on + strand at 2896.750 kb on + strand at 2896.858 kb on + strand at 2896.883 kb on - strand at 2896.900 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 6 days starvation remove 2,894,512 + -3.4 2,894,553 + -6.1 2,894,615 - DvMF_2304 0.15 -6.3 2,894,896 + DvMF_2304 0.51 -3.8 2,895,003 + DvMF_2304 0.65 -5.2 2,895,005 - DvMF_2304 0.65 -3.9 2,895,216 + -6.4 2,895,282 - -4.6 2,895,411 + hisH DvMF_2305 0.23 -5.7 2,895,738 - hisH DvMF_2305 0.74 -7.3 2,895,840 - hisH DvMF_2305 0.90 -4.8 2,895,896 - -8.2 2,895,958 + -0.2 2,896,166 + -1.0 2,896,658 + -5.2 2,896,750 + -3.6 2,896,750 + -7.3 2,896,858 + -5.2 2,896,883 - -5.6 2,896,900 + -6.5
Or see this region's nucleotide sequence