Experiment: Glycine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_3200 and Shewana3_3201 are separated by 385 nucleotides Shewana3_3201 and Shewana3_3202 are separated by 46 nucleotides Shewana3_3202 and djlA are separated by 55 nucleotides
Shewana3_3200: Shewana3_3200 - hypothetical protein (RefSeq), at 3,842,938 to 3,843,972
_3200
Shewana3_3201: Shewana3_3201 - D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding (RefSeq), at 3,844,358 to 3,845,311
_3201
Shewana3_3202: Shewana3_3202 - hypothetical protein (RefSeq), at 3,845,358 to 3,846,002
_3202
Shewana3_3203: djlA - Dna-J like membrane chaperone protein (RefSeq), at 3,846,058 to 3,846,846
djlA
Position (kb)
3844
3845
3846 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3843.387 kb on + strand, within Shewana3_3200 at 3843.482 kb on - strand, within Shewana3_3200 at 3843.948 kb on - strand at 3843.984 kb on - strand at 3844.124 kb on - strand at 3844.222 kb on - strand at 3844.687 kb on + strand, within Shewana3_3201 at 3844.873 kb on - strand, within Shewana3_3201 at 3844.944 kb on + strand, within Shewana3_3201 at 3844.944 kb on + strand, within Shewana3_3201 at 3844.952 kb on - strand, within Shewana3_3201 at 3845.041 kb on + strand, within Shewana3_3201 at 3845.056 kb on - strand, within Shewana3_3201 at 3845.088 kb on - strand, within Shewana3_3201 at 3845.706 kb on - strand, within Shewana3_3202 at 3845.910 kb on - strand, within Shewana3_3202 at 3845.952 kb on + strand at 3845.959 kb on - strand at 3846.088 kb on + strand at 3846.088 kb on + strand at 3846.088 kb on + strand at 3846.096 kb on - strand at 3846.137 kb on + strand, within djlA
Per-strain Table
Position Strand Gene LocusTag Fraction Glycine (N) remove 3,843,387 + Shewana3_3200 0.43 +0.2 3,843,482 - Shewana3_3200 0.53 +0.8 3,843,948 - -0.9 3,843,984 - -0.6 3,844,124 - -0.1 3,844,222 - +0.8 3,844,687 + Shewana3_3201 0.34 +0.8 3,844,873 - Shewana3_3201 0.54 +0.4 3,844,944 + Shewana3_3201 0.61 -1.2 3,844,944 + Shewana3_3201 0.61 +0.4 3,844,952 - Shewana3_3201 0.62 -0.1 3,845,041 + Shewana3_3201 0.72 +0.6 3,845,056 - Shewana3_3201 0.73 -1.0 3,845,088 - Shewana3_3201 0.77 +1.0 3,845,706 - Shewana3_3202 0.54 -0.4 3,845,910 - Shewana3_3202 0.86 +0.2 3,845,952 + +1.3 3,845,959 - -1.0 3,846,088 + -0.2 3,846,088 + +1.0 3,846,088 + +0.8 3,846,096 - +2.9 3,846,137 + djlA Shewana3_3203 0.10 -0.1
Or see this region's nucleotide sequence