Experiment: Glycine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_3033 and Shewana3_3034 are separated by 90 nucleotides Shewana3_3034 and Shewana3_3035 overlap by 20 nucleotides Shewana3_3035 and nhaR are separated by 91 nucleotides
Shewana3_3033: Shewana3_3033 - L-aspartate oxidase (RefSeq), at 3,638,013 to 3,639,626
_3033
Shewana3_3034: Shewana3_3034 - hypothetical protein (RefSeq), at 3,639,717 to 3,640,157
_3034
Shewana3_3035: Shewana3_3035 - hypothetical protein (RefSeq), at 3,640,138 to 3,640,377
_3035
Shewana3_3036: nhaR - transcriptional activator NhaR (RefSeq), at 3,640,469 to 3,641,377
nhaR
Position (kb)
3640
3641 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3639.663 kb on - strand at 3639.670 kb on + strand at 3639.861 kb on + strand, within Shewana3_3034 at 3640.198 kb on + strand, within Shewana3_3035 at 3640.206 kb on - strand, within Shewana3_3035 at 3640.336 kb on - strand, within Shewana3_3035 at 3640.424 kb on + strand at 3640.865 kb on + strand, within nhaR at 3641.137 kb on + strand, within nhaR at 3641.276 kb on + strand, within nhaR at 3641.290 kb on + strand at 3641.298 kb on - strand at 3641.305 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Glycine (N) remove 3,639,663 - -2.4 3,639,670 + +0.0 3,639,861 + Shewana3_3034 0.33 -0.4 3,640,198 + Shewana3_3035 0.25 -2.1 3,640,206 - Shewana3_3035 0.28 -0.8 3,640,336 - Shewana3_3035 0.82 -2.2 3,640,424 + +1.9 3,640,865 + nhaR Shewana3_3036 0.44 +0.9 3,641,137 + nhaR Shewana3_3036 0.73 -0.6 3,641,276 + nhaR Shewana3_3036 0.89 +0.4 3,641,290 + +0.5 3,641,298 - -1.1 3,641,305 - -1.2
Or see this region's nucleotide sequence