Experiment: Glycine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tyrA and Shewana3_3013 are separated by 62 nucleotides Shewana3_3013 and rplS are separated by 297 nucleotides rplS and trmD are separated by 34 nucleotides
Shewana3_3012: tyrA - bifunctional chorismate mutase/prephenate dehydrogenase (RefSeq), at 3,619,096 to 3,620,235
tyrA
Shewana3_3013: Shewana3_3013 - phospho-2-dehydro-3-deoxyheptonate aldolase (RefSeq), at 3,620,298 to 3,621,389
_3013
Shewana3_3014: rplS - 50S ribosomal protein L19 (RefSeq), at 3,621,687 to 3,622,040
rplS
Shewana3_3015: trmD - tRNA (guanine-N(1)-)-methyltransferase (RefSeq), at 3,622,075 to 3,622,821
trmD
Position (kb)
3620
3621
3622 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3619.844 kb on + strand, within tyrA at 3619.903 kb on - strand, within tyrA at 3620.112 kb on + strand, within tyrA at 3620.420 kb on + strand, within Shewana3_3013 at 3620.568 kb on - strand, within Shewana3_3013 at 3620.568 kb on - strand, within Shewana3_3013 at 3620.652 kb on + strand, within Shewana3_3013 at 3620.660 kb on - strand, within Shewana3_3013 at 3620.811 kb on - strand, within Shewana3_3013 at 3621.032 kb on - strand, within Shewana3_3013 at 3621.169 kb on - strand, within Shewana3_3013
Per-strain Table
Position Strand Gene LocusTag Fraction Glycine (N) remove 3,619,844 + tyrA Shewana3_3012 0.66 -3.6 3,619,903 - tyrA Shewana3_3012 0.71 -4.2 3,620,112 + tyrA Shewana3_3012 0.89 -2.9 3,620,420 + Shewana3_3013 0.11 -4.1 3,620,568 - Shewana3_3013 0.25 -2.6 3,620,568 - Shewana3_3013 0.25 -3.2 3,620,652 + Shewana3_3013 0.32 -2.6 3,620,660 - Shewana3_3013 0.33 -0.9 3,620,811 - Shewana3_3013 0.47 -2.3 3,621,032 - Shewana3_3013 0.67 -1.3 3,621,169 - Shewana3_3013 0.80 -2.7
Or see this region's nucleotide sequence