Experiment: Glycine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2383 and minE are separated by 2 nucleotides minE and Shewana3_2385 are separated by 162 nucleotides Shewana3_2385 and Shewana3_2386 are separated by 14 nucleotides
Shewana3_2383: Shewana3_2383 - septum site-determining protein MinD (RefSeq), at 2,862,499 to 2,863,308
_2383
Shewana3_2384: minE - cell division topological specificity factor MinE (RefSeq), at 2,863,311 to 2,863,568
minE
Shewana3_2385: Shewana3_2385 - ribonuclease D (RefSeq), at 2,863,731 to 2,864,897
_2385
Shewana3_2386: Shewana3_2386 - long-chain-fatty-acid--CoA ligase (RefSeq), at 2,864,912 to 2,866,585
_2386
Position (kb)
2863
2864
2865 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2862.926 kb on + strand, within Shewana3_2383 at 2863.079 kb on + strand, within Shewana3_2383 at 2863.526 kb on + strand, within minE at 2863.669 kb on + strand at 2863.677 kb on - strand at 2864.253 kb on - strand, within Shewana3_2385 at 2864.386 kb on + strand, within Shewana3_2385 at 2864.388 kb on + strand, within Shewana3_2385 at 2864.487 kb on + strand, within Shewana3_2385 at 2864.495 kb on - strand, within Shewana3_2385 at 2864.550 kb on - strand, within Shewana3_2385 at 2864.635 kb on + strand, within Shewana3_2385 at 2864.687 kb on - strand, within Shewana3_2385 at 2864.717 kb on + strand, within Shewana3_2385 at 2864.870 kb on - strand at 2865.400 kb on - strand, within Shewana3_2386 at 2865.875 kb on - strand, within Shewana3_2386
Per-strain Table
Position Strand Gene LocusTag Fraction Glycine (N) remove 2,862,926 + Shewana3_2383 0.53 -0.3 2,863,079 + Shewana3_2383 0.72 +0.3 2,863,526 + minE Shewana3_2384 0.83 -0.6 2,863,669 + -0.2 2,863,677 - -0.7 2,864,253 - Shewana3_2385 0.45 -1.7 2,864,386 + Shewana3_2385 0.56 -0.3 2,864,388 + Shewana3_2385 0.56 -2.8 2,864,487 + Shewana3_2385 0.65 -0.6 2,864,495 - Shewana3_2385 0.65 -0.0 2,864,550 - Shewana3_2385 0.70 -0.7 2,864,635 + Shewana3_2385 0.77 -0.3 2,864,687 - Shewana3_2385 0.82 -0.4 2,864,717 + Shewana3_2385 0.84 -1.9 2,864,870 - +0.4 2,865,400 - Shewana3_2386 0.29 -0.6 2,865,875 - Shewana3_2386 0.58 +0.4
Or see this region's nucleotide sequence