Experiment: Glycine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2365 and rnhA are separated by 70 nucleotides rnhA and Shewana3_2367 are separated by 36 nucleotides Shewana3_2367 and Shewana3_2368 are separated by 23 nucleotides
Shewana3_2365: Shewana3_2365 - DNA polymerase III, epsilon subunit (RefSeq), at 2,844,011 to 2,844,739
_2365
Shewana3_2366: rnhA - ribonuclease H (RefSeq), at 2,844,810 to 2,845,286
rnhA
Shewana3_2367: Shewana3_2367 - LysR family transcriptional regulator (RefSeq), at 2,845,323 to 2,846,222
_2367
Shewana3_2368: Shewana3_2368 - methyltransferase type 11 (RefSeq), at 2,846,246 to 2,846,980
_2368
Position (kb)
2844
2845
2846 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2843.837 kb on + strand at 2843.837 kb on + strand at 2844.870 kb on - strand, within rnhA at 2845.080 kb on + strand, within rnhA at 2845.433 kb on - strand, within Shewana3_2367 at 2845.438 kb on - strand, within Shewana3_2367 at 2845.471 kb on - strand, within Shewana3_2367 at 2845.506 kb on + strand, within Shewana3_2367
Per-strain Table
Position Strand Gene LocusTag Fraction Glycine (N) remove 2,843,837 + +0.5 2,843,837 + -0.4 2,844,870 - rnhA Shewana3_2366 0.13 -0.8 2,845,080 + rnhA Shewana3_2366 0.57 -2.0 2,845,433 - Shewana3_2367 0.12 -1.1 2,845,438 - Shewana3_2367 0.13 -0.2 2,845,471 - Shewana3_2367 0.16 +1.1 2,845,506 + Shewana3_2367 0.20 +3.6
Or see this region's nucleotide sequence