Experiment: Glycine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2052 and Shewana3_2053 overlap by 8 nucleotides Shewana3_2053 and Shewana3_2054 are separated by 18 nucleotides Shewana3_2054 and Shewana3_2055 are separated by 18 nucleotides Shewana3_2055 and Shewana3_2056 are separated by 67 nucleotides Shewana3_2056 and Shewana3_2057 overlap by 7 nucleotides Shewana3_2057 and Shewana3_2058 overlap by 1 nucleotides
Shewana3_2052: Shewana3_2052 - recombination factor protein RarA (RefSeq), at 2,461,370 to 2,462,701
_2052
Shewana3_2053: Shewana3_2053 - camphor resistance protein CrcB (RefSeq), at 2,462,694 to 2,463,068
_2053
Shewana3_2054: Shewana3_2054 - seryl-tRNA synthetase (RefSeq), at 2,463,087 to 2,464,373
_2054
Shewana3_2055: Shewana3_2055 - hypothetical protein (RefSeq), at 2,464,392 to 2,464,514
_2055
Shewana3_2056: Shewana3_2056 - formate dehydrogenase (RefSeq), at 2,464,582 to 2,464,920
_2056
Shewana3_2057: Shewana3_2057 - DsrH family protein (RefSeq), at 2,464,914 to 2,465,195
_2057
Shewana3_2058: Shewana3_2058 - DsrE family protein (RefSeq), at 2,465,195 to 2,465,551
_2058
Position (kb)
2463
2464
2465 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2462.307 kb on - strand, within Shewana3_2052 at 2462.584 kb on + strand at 2462.795 kb on + strand, within Shewana3_2053 at 2464.382 kb on + strand at 2464.407 kb on + strand, within Shewana3_2055 at 2464.475 kb on + strand, within Shewana3_2055 at 2464.554 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Glycine (N) remove 2,462,307 - Shewana3_2052 0.70 -0.6 2,462,584 + +1.0 2,462,795 + Shewana3_2053 0.27 +1.0 2,464,382 + +3.4 2,464,407 + Shewana3_2055 0.12 +1.8 2,464,475 + Shewana3_2055 0.67 -0.6 2,464,554 - +1.3
Or see this region's nucleotide sequence