Experiment: Glycine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0545 and Shewana3_0546 are separated by 62 nucleotides Shewana3_0546 and Shewana3_0547 are separated by 289 nucleotides Shewana3_0547 and Shewana3_0548 are separated by 146 nucleotides
Shewana3_0545: Shewana3_0545 - alpha-L-glutamate ligase (RefSeq), at 640,850 to 641,755
_0545
Shewana3_0546: Shewana3_0546 - hypothetical protein (RefSeq), at 641,818 to 642,510
_0546
Shewana3_0547: Shewana3_0547 - histone family protein DNA-binding protein (RefSeq), at 642,800 to 643,072
_0547
Shewana3_0548: Shewana3_0548 - no description, at 643,219 to 644,016
_0548
Position (kb) 641
642
643 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2
3 at 640.890 kb on - strand at 641.188 kb on - strand, within Shewana3_0545 at 641.188 kb on - strand, within Shewana3_0545 at 641.236 kb on + strand, within Shewana3_0545 at 641.486 kb on + strand, within Shewana3_0545 at 641.526 kb on + strand, within Shewana3_0545 at 641.571 kb on + strand, within Shewana3_0545 at 641.579 kb on - strand, within Shewana3_0545 at 641.655 kb on + strand, within Shewana3_0545 at 642.001 kb on + strand, within Shewana3_0546 at 642.192 kb on + strand, within Shewana3_0546 at 642.216 kb on + strand, within Shewana3_0546 at 642.258 kb on - strand, within Shewana3_0546 at 642.260 kb on + strand, within Shewana3_0546 at 642.260 kb on + strand, within Shewana3_0546 at 642.266 kb on + strand, within Shewana3_0546 at 642.358 kb on + strand, within Shewana3_0546 at 642.522 kb on + strand at 642.564 kb on + strand at 642.831 kb on + strand, within Shewana3_0547 at 642.859 kb on - strand, within Shewana3_0547 at 643.065 kb on - strand at 643.124 kb on + strand at 643.280 kb on - strand at 643.280 kb on - strand at 643.300 kb on - strand, within Shewana3_0548 at 643.388 kb on + strand, within Shewana3_0548 at 643.406 kb on - strand, within Shewana3_0548
Per-strain Table
Position Strand Gene LocusTag Fraction Glycine (N) remove 640,890 - +0.7 641,188 - Shewana3_0545 0.37 -1.2 641,188 - Shewana3_0545 0.37 +0.7 641,236 + Shewana3_0545 0.43 -0.4 641,486 + Shewana3_0545 0.70 +0.7 641,526 + Shewana3_0545 0.75 +1.1 641,571 + Shewana3_0545 0.80 +0.5 641,579 - Shewana3_0545 0.80 -0.8 641,655 + Shewana3_0545 0.89 -1.0 642,001 + Shewana3_0546 0.26 -2.0 642,192 + Shewana3_0546 0.54 -0.7 642,216 + Shewana3_0546 0.57 -1.5 642,258 - Shewana3_0546 0.63 +0.1 642,260 + Shewana3_0546 0.64 +0.2 642,260 + Shewana3_0546 0.64 -6.5 642,266 + Shewana3_0546 0.65 +0.6 642,358 + Shewana3_0546 0.78 -1.1 642,522 + +0.4 642,564 + +0.4 642,831 + Shewana3_0547 0.11 +1.5 642,859 - Shewana3_0547 0.22 -3.2 643,065 - -0.1 643,124 + +0.7 643,280 - +0.1 643,280 - +0.2 643,300 - Shewana3_0548 0.10 +3.6 643,388 + Shewana3_0548 0.21 +0.3 643,406 - Shewana3_0548 0.23 -0.5
Or see this region's nucleotide sequence