Experiment: Glycine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0022 and fadA are separated by 224 nucleotides fadA and fadB are separated by 22 nucleotides
Shewana3_0022: Shewana3_0022 - diguanylate cyclase (RefSeq), at 26,815 to 28,026
_0022
Shewana3_0023: fadA - Acetyl-CoA C-acyltransferase (EC 2.3.1.16) <small>(from data)</small>, at 28,251 to 29,414
fadA
Shewana3_0024: fadB - Enoyl-CoA hydratase (EC 4.2.1.17); 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) <small>(from data)</small>, at 29,437 to 31,587
fadB
Position (kb) 28
29
30 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 27.279 kb on - strand, within Shewana3_0022 at 27.314 kb on - strand, within Shewana3_0022 at 27.315 kb on + strand, within Shewana3_0022 at 27.340 kb on + strand, within Shewana3_0022 at 27.340 kb on + strand, within Shewana3_0022 at 27.340 kb on + strand, within Shewana3_0022 at 27.348 kb on - strand, within Shewana3_0022 at 27.381 kb on + strand, within Shewana3_0022 at 27.438 kb on + strand, within Shewana3_0022 at 27.446 kb on - strand, within Shewana3_0022 at 27.763 kb on - strand, within Shewana3_0022 at 27.763 kb on - strand, within Shewana3_0022 at 27.763 kb on - strand, within Shewana3_0022 at 27.763 kb on - strand, within Shewana3_0022 at 27.831 kb on + strand, within Shewana3_0022 at 27.850 kb on + strand, within Shewana3_0022 at 27.978 kb on + strand at 28.041 kb on - strand at 28.069 kb on + strand at 28.069 kb on + strand at 28.069 kb on + strand at 28.071 kb on + strand at 28.077 kb on - strand at 28.077 kb on - strand at 28.077 kb on - strand at 28.143 kb on - strand at 28.169 kb on + strand at 28.249 kb on + strand at 28.256 kb on - strand at 28.283 kb on + strand at 28.283 kb on + strand at 28.292 kb on - strand at 28.375 kb on - strand, within fadA at 28.377 kb on + strand, within fadA at 28.385 kb on - strand, within fadA at 28.418 kb on - strand, within fadA at 28.428 kb on - strand, within fadA at 28.438 kb on - strand, within fadA at 28.445 kb on + strand, within fadA at 28.445 kb on + strand, within fadA at 28.454 kb on + strand, within fadA at 28.464 kb on + strand, within fadA at 28.473 kb on - strand, within fadA at 28.473 kb on - strand, within fadA at 28.473 kb on - strand, within fadA at 28.734 kb on + strand, within fadA at 28.742 kb on - strand, within fadA at 28.804 kb on + strand, within fadA at 28.841 kb on - strand, within fadA at 28.874 kb on - strand, within fadA at 28.903 kb on - strand, within fadA at 28.931 kb on + strand, within fadA at 28.947 kb on - strand, within fadA at 29.012 kb on - strand, within fadA at 29.042 kb on - strand, within fadA at 29.065 kb on - strand, within fadA at 29.220 kb on - strand, within fadA at 29.222 kb on - strand, within fadA at 29.225 kb on + strand, within fadA at 29.233 kb on - strand, within fadA at 29.233 kb on - strand, within fadA at 29.289 kb on - strand, within fadA at 29.297 kb on + strand, within fadA at 29.297 kb on + strand, within fadA at 29.305 kb on - strand at 29.370 kb on + strand at 29.370 kb on + strand at 29.378 kb on - strand at 29.378 kb on - strand at 29.378 kb on - strand at 29.458 kb on - strand at 29.512 kb on - strand at 29.560 kb on - strand at 29.580 kb on - strand at 29.614 kb on - strand at 29.655 kb on + strand, within fadB at 29.663 kb on - strand, within fadB at 29.839 kb on - strand, within fadB at 29.976 kb on - strand, within fadB at 30.174 kb on + strand, within fadB at 30.174 kb on + strand, within fadB at 30.179 kb on + strand, within fadB at 30.182 kb on - strand, within fadB at 30.200 kb on + strand, within fadB at 30.227 kb on + strand, within fadB at 30.227 kb on + strand, within fadB at 30.235 kb on - strand, within fadB at 30.235 kb on - strand, within fadB at 30.235 kb on - strand, within fadB at 30.235 kb on - strand, within fadB
Per-strain Table
Position Strand Gene LocusTag Fraction Glycine (N) remove 27,279 - Shewana3_0022 0.38 -2.3 27,314 - Shewana3_0022 0.41 +2.7 27,315 + Shewana3_0022 0.41 +0.5 27,340 + Shewana3_0022 0.43 +0.7 27,340 + Shewana3_0022 0.43 +3.2 27,340 + Shewana3_0022 0.43 -1.5 27,348 - Shewana3_0022 0.44 +0.3 27,381 + Shewana3_0022 0.47 -1.2 27,438 + Shewana3_0022 0.51 +0.9 27,446 - Shewana3_0022 0.52 -1.2 27,763 - Shewana3_0022 0.78 -0.7 27,763 - Shewana3_0022 0.78 -0.1 27,763 - Shewana3_0022 0.78 -1.6 27,763 - Shewana3_0022 0.78 -2.0 27,831 + Shewana3_0022 0.84 -1.0 27,850 + Shewana3_0022 0.85 +1.6 27,978 + -0.8 28,041 - -1.6 28,069 + +0.1 28,069 + -1.2 28,069 + +0.2 28,071 + +0.4 28,077 - +0.4 28,077 - +0.6 28,077 - +1.6 28,143 - +0.0 28,169 + -0.7 28,249 + +0.4 28,256 - -1.1 28,283 + +0.5 28,283 + -1.6 28,292 - -0.1 28,375 - fadA Shewana3_0023 0.11 +0.3 28,377 + fadA Shewana3_0023 0.11 +0.4 28,385 - fadA Shewana3_0023 0.12 +0.2 28,418 - fadA Shewana3_0023 0.14 -0.0 28,428 - fadA Shewana3_0023 0.15 +0.8 28,438 - fadA Shewana3_0023 0.16 -1.7 28,445 + fadA Shewana3_0023 0.17 -2.3 28,445 + fadA Shewana3_0023 0.17 -0.5 28,454 + fadA Shewana3_0023 0.17 +0.5 28,464 + fadA Shewana3_0023 0.18 +0.4 28,473 - fadA Shewana3_0023 0.19 -0.1 28,473 - fadA Shewana3_0023 0.19 +2.0 28,473 - fadA Shewana3_0023 0.19 -1.0 28,734 + fadA Shewana3_0023 0.41 +1.7 28,742 - fadA Shewana3_0023 0.42 +0.2 28,804 + fadA Shewana3_0023 0.48 -1.3 28,841 - fadA Shewana3_0023 0.51 -0.4 28,874 - fadA Shewana3_0023 0.54 -1.4 28,903 - fadA Shewana3_0023 0.56 +0.4 28,931 + fadA Shewana3_0023 0.58 -1.1 28,947 - fadA Shewana3_0023 0.60 +0.7 29,012 - fadA Shewana3_0023 0.65 +0.6 29,042 - fadA Shewana3_0023 0.68 +1.5 29,065 - fadA Shewana3_0023 0.70 +0.4 29,220 - fadA Shewana3_0023 0.83 -0.6 29,222 - fadA Shewana3_0023 0.83 +0.2 29,225 + fadA Shewana3_0023 0.84 +1.2 29,233 - fadA Shewana3_0023 0.84 -1.2 29,233 - fadA Shewana3_0023 0.84 +0.2 29,289 - fadA Shewana3_0023 0.89 -0.6 29,297 + fadA Shewana3_0023 0.90 +1.5 29,297 + fadA Shewana3_0023 0.90 +0.2 29,305 - +0.8 29,370 + -1.3 29,370 + -0.6 29,378 - -1.0 29,378 - +0.7 29,378 - +0.5 29,458 - +0.4 29,512 - -2.4 29,560 - +2.1 29,580 - -1.2 29,614 - +0.1 29,655 + fadB Shewana3_0024 0.10 -1.5 29,663 - fadB Shewana3_0024 0.11 -1.5 29,839 - fadB Shewana3_0024 0.19 -0.9 29,976 - fadB Shewana3_0024 0.25 -0.7 30,174 + fadB Shewana3_0024 0.34 -1.0 30,174 + fadB Shewana3_0024 0.34 +0.0 30,179 + fadB Shewana3_0024 0.34 -0.1 30,182 - fadB Shewana3_0024 0.35 -0.2 30,200 + fadB Shewana3_0024 0.35 -2.1 30,227 + fadB Shewana3_0024 0.37 -1.6 30,227 + fadB Shewana3_0024 0.37 -1.3 30,235 - fadB Shewana3_0024 0.37 +2.2 30,235 - fadB Shewana3_0024 0.37 +0.5 30,235 - fadB Shewana3_0024 0.37 -1.4 30,235 - fadB Shewana3_0024 0.37 +0.9
Or see this region's nucleotide sequence