Experiment: casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2421 and Shew_2422 are separated by 150 nucleotides Shew_2422 and Shew_2423 are separated by 18 nucleotides Shew_2423 and Shew_2424 are separated by 293 nucleotides Shew_2424 and fadJ are separated by 158 nucleotides
Shew_2421: Shew_2421 - diguanylate cyclase/phosphodiesterase with PAS/PAC and GAF sensor(s) (RefSeq), at 2,843,433 to 2,847,746
_2421
Shew_2422: Shew_2422 - hypothetical protein (RefSeq), at 2,847,897 to 2,848,532
_2422
Shew_2423: Shew_2423 - hypothetical protein (RefSeq), at 2,848,551 to 2,848,670
_2423
Shew_2424: Shew_2424 - hypothetical protein (RefSeq), at 2,848,964 to 2,849,170
_2424
Shew_2425: fadJ - multifunctional fatty acid oxidation complex subunit alpha (RefSeq), at 2,849,329 to 2,851,449
fadJ
Position (kb)
2847
2848
2849 Strain fitness (log2 ratio)
-1
0
1 at 2846.981 kb on - strand, within Shew_2421 at 2846.981 kb on - strand, within Shew_2421 at 2846.997 kb on + strand, within Shew_2421 at 2847.100 kb on + strand, within Shew_2421 at 2847.408 kb on - strand at 2847.408 kb on + strand at 2847.527 kb on - strand at 2848.360 kb on - strand, within Shew_2422 at 2848.621 kb on - strand, within Shew_2423 at 2848.819 kb on - strand at 2848.855 kb on + strand at 2848.954 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction casaminos (C) remove 2,846,981 - Shew_2421 0.82 +0.2 2,846,981 - Shew_2421 0.82 +0.1 2,846,997 + Shew_2421 0.83 +0.0 2,847,100 + Shew_2421 0.85 +0.1 2,847,408 - +0.4 2,847,408 + +0.2 2,847,527 - -0.0 2,848,360 - Shew_2422 0.73 +0.6 2,848,621 - Shew_2423 0.58 +0.7 2,848,819 - -0.0 2,848,855 + -0.3 2,848,954 - +0.5
Or see this region's nucleotide sequence