Experiment: casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2051 and Shew_2052 are separated by 8 nucleotides Shew_2052 and Shew_2053 are separated by 219 nucleotides Shew_2053 and Shew_2054 are separated by 154 nucleotides
Shew_2051: Shew_2051 - acyl-CoA dehydrogenase (RefSeq), at 2,368,011 to 2,370,287
_2051
Shew_2052: Shew_2052 - TetR family transcriptional regulator (RefSeq), at 2,370,296 to 2,370,934
_2052
Shew_2053: Shew_2053 - peptidyl-dipeptidase A (RefSeq), at 2,371,154 to 2,373,058
_2053
Shew_2054: Shew_2054 - Smr protein/MutS2 (RefSeq), at 2,373,213 to 2,373,803
_2054
Position (kb)
2371
2372
2373
2374 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2370.273 kb on + strand at 2370.418 kb on - strand, within Shew_2052 at 2370.593 kb on + strand, within Shew_2052 at 2371.661 kb on + strand, within Shew_2053 at 2371.689 kb on + strand, within Shew_2053 at 2372.072 kb on + strand, within Shew_2053 at 2372.576 kb on + strand, within Shew_2053 at 2372.669 kb on - strand, within Shew_2053 at 2372.818 kb on + strand, within Shew_2053 at 2372.906 kb on - strand at 2372.917 kb on + strand at 2372.917 kb on + strand at 2372.917 kb on + strand at 2372.945 kb on - strand at 2372.973 kb on + strand at 2373.413 kb on + strand, within Shew_2054 at 2373.719 kb on + strand, within Shew_2054 at 2373.760 kb on - strand at 2373.938 kb on - strand at 2373.938 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction casaminos (C) remove 2,370,273 + -2.4 2,370,418 - Shew_2052 0.19 -1.0 2,370,593 + Shew_2052 0.46 +1.5 2,371,661 + Shew_2053 0.27 +0.5 2,371,689 + Shew_2053 0.28 +0.4 2,372,072 + Shew_2053 0.48 +0.1 2,372,576 + Shew_2053 0.75 +0.5 2,372,669 - Shew_2053 0.80 +3.1 2,372,818 + Shew_2053 0.87 +1.2 2,372,906 - -0.4 2,372,917 + +0.6 2,372,917 + -0.3 2,372,917 + +0.6 2,372,945 - +0.4 2,372,973 + -0.1 2,373,413 + Shew_2054 0.34 -1.8 2,373,719 + Shew_2054 0.86 +0.4 2,373,760 - -0.3 2,373,938 - +1.1 2,373,938 - +1.5
Or see this region's nucleotide sequence