Experiment: casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1258 and Shew_1259 are separated by 284 nucleotides Shew_1259 and Shew_1260 are separated by 370 nucleotides Shew_1260 and Shew_1261 are separated by 5 nucleotides
Shew_1258: Shew_1258 - phage transcriptional regulator, AlpA (RefSeq), at 1,454,598 to 1,454,783
_1258
Shew_1259: Shew_1259 - phage transcriptional regulator, AlpA (RefSeq), at 1,455,068 to 1,455,226
_1259
Shew_1260: Shew_1260 - DNA methylase N-4/N-6 domain-containing protein (RefSeq), at 1,455,597 to 1,457,756
_1260
Shew_1261: Shew_1261 - type III restriction enzyme, res subunit (RefSeq), at 1,457,762 to 1,460,347
_1261
Position (kb)
1455
1456
1457
1458 Strain fitness (log2 ratio)
-1
0
1 at 1454.673 kb on - strand, within Shew_1258 at 1454.958 kb on - strand at 1455.085 kb on + strand, within Shew_1259 at 1455.118 kb on + strand, within Shew_1259 at 1455.383 kb on + strand at 1455.391 kb on - strand at 1455.565 kb on + strand at 1455.581 kb on - strand at 1455.626 kb on + strand at 1455.733 kb on + strand at 1455.830 kb on + strand, within Shew_1260 at 1456.060 kb on - strand, within Shew_1260 at 1456.100 kb on + strand, within Shew_1260 at 1456.734 kb on + strand, within Shew_1260 at 1456.837 kb on + strand, within Shew_1260 at 1457.340 kb on - strand, within Shew_1260 at 1457.692 kb on + strand at 1457.884 kb on + strand at 1458.270 kb on + strand, within Shew_1261
Per-strain Table
Position Strand Gene LocusTag Fraction casaminos (C) remove 1,454,673 - Shew_1258 0.40 -0.1 1,454,958 - +0.0 1,455,085 + Shew_1259 0.11 -0.4 1,455,118 + Shew_1259 0.31 +0.2 1,455,383 + +0.9 1,455,391 - +0.1 1,455,565 + -0.1 1,455,581 - +0.2 1,455,626 + -0.4 1,455,733 + -0.8 1,455,830 + Shew_1260 0.11 -0.3 1,456,060 - Shew_1260 0.21 -0.8 1,456,100 + Shew_1260 0.23 -0.6 1,456,734 + Shew_1260 0.53 -0.5 1,456,837 + Shew_1260 0.57 -0.1 1,457,340 - Shew_1260 0.81 -0.0 1,457,692 + -0.2 1,457,884 + +0.1 1,458,270 + Shew_1261 0.20 +0.1
Or see this region's nucleotide sequence