Experiment: casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0101 and Shew_0102 are separated by 101 nucleotides Shew_0102 and Shew_0103 are separated by 206 nucleotides Shew_0103 and Shew_0104 are separated by 52 nucleotides Shew_0104 and Shew_0105 are separated by 75 nucleotides
Shew_0101: Shew_0101 - endoribonuclease L-PSP (RefSeq), at 111,320 to 111,721
_0101
Shew_0102: Shew_0102 - hypothetical protein (RefSeq), at 111,823 to 111,978
_0102
Shew_0103: Shew_0103 - sodium/glutamate symporter (RefSeq), at 112,185 to 113,408
_0103
Shew_0104: Shew_0104 - hypothetical protein (RefSeq), at 113,461 to 113,712
_0104
Shew_0105: Shew_0105 - peptidase M14, carboxypeptidase A (RefSeq), at 113,788 to 116,325
_0105
Position (kb) 112
113
114 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 111.225 kb on + strand at 111.413 kb on - strand, within Shew_0101 at 111.429 kb on + strand, within Shew_0101 at 111.450 kb on - strand, within Shew_0101 at 111.493 kb on + strand, within Shew_0101 at 111.745 kb on + strand at 111.879 kb on + strand, within Shew_0102 at 112.067 kb on - strand at 112.101 kb on - strand at 113.001 kb on - strand, within Shew_0103 at 113.193 kb on - strand, within Shew_0103 at 113.321 kb on - strand at 113.688 kb on + strand at 113.698 kb on - strand at 113.821 kb on + strand at 113.936 kb on - strand at 114.073 kb on - strand, within Shew_0105 at 114.378 kb on + strand, within Shew_0105
Per-strain Table
Position Strand Gene LocusTag Fraction casaminos (C) remove 111,225 + -0.1 111,413 - Shew_0101 0.23 +0.3 111,429 + Shew_0101 0.27 +0.5 111,450 - Shew_0101 0.32 -0.4 111,493 + Shew_0101 0.43 +0.1 111,745 + +0.3 111,879 + Shew_0102 0.36 +0.7 112,067 - -0.4 112,101 - +0.1 113,001 - Shew_0103 0.67 -0.4 113,193 - Shew_0103 0.82 -2.9 113,321 - -0.5 113,688 + -0.1 113,698 - -0.0 113,821 + -0.0 113,936 - +0.2 114,073 - Shew_0105 0.11 +0.3 114,378 + Shew_0105 0.23 -1.0
Or see this region's nucleotide sequence