Experiment: Entrained for one LD cycle; Darkness for 72h then Flask growth in 60 uE constant light
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_2097 and rfbC are separated by 65 nucleotides rfbC and rfbD overlap by 4 nucleotides rfbD and rfbB overlap by 4 nucleotides
Synpcc7942_2097: Synpcc7942_2097 - hypothetical protein, at 2,179,471 to 2,180,391
_2097
Synpcc7942_2098: rfbC - dTDP-4-dehydrorhamnose 3,5-epimerase, at 2,180,457 to 2,181,008
rfbC
Synpcc7942_2099: rfbD - dTDP-4-dehydrorhamnose reductase, at 2,181,005 to 2,181,874
rfbD
Synpcc7942_2100: rfbB - dTDP-glucose 4,6-dehydratase, at 2,181,871 to 2,182,944
rfbB
Position (kb)
2181
2182 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2180.021 kb on + strand, within Synpcc7942_2097 at 2180.061 kb on + strand, within Synpcc7942_2097 at 2180.063 kb on + strand, within Synpcc7942_2097 at 2180.069 kb on - strand, within Synpcc7942_2097 at 2180.071 kb on + strand, within Synpcc7942_2097 at 2180.071 kb on + strand, within Synpcc7942_2097 at 2180.079 kb on - strand, within Synpcc7942_2097 at 2180.079 kb on - strand, within Synpcc7942_2097 at 2180.079 kb on - strand, within Synpcc7942_2097 at 2180.092 kb on + strand, within Synpcc7942_2097 at 2180.148 kb on - strand, within Synpcc7942_2097 at 2180.230 kb on + strand, within Synpcc7942_2097 at 2180.238 kb on - strand, within Synpcc7942_2097 at 2180.257 kb on - strand, within Synpcc7942_2097 at 2180.257 kb on - strand, within Synpcc7942_2097 at 2180.290 kb on + strand, within Synpcc7942_2097 at 2180.298 kb on - strand, within Synpcc7942_2097 at 2180.304 kb on - strand at 2180.319 kb on + strand at 2180.344 kb on - strand at 2180.423 kb on - strand at 2180.428 kb on - strand at 2180.428 kb on - strand at 2180.435 kb on - strand at 2180.462 kb on - strand at 2180.494 kb on - strand at 2180.542 kb on + strand, within rfbC at 2180.677 kb on + strand, within rfbC at 2180.685 kb on - strand, within rfbC at 2180.713 kb on + strand, within rfbC at 2180.734 kb on + strand, within rfbC at 2180.754 kb on - strand, within rfbC at 2180.793 kb on + strand, within rfbC at 2180.891 kb on + strand, within rfbC at 2180.899 kb on - strand, within rfbC at 2180.946 kb on + strand, within rfbC at 2180.954 kb on - strand at 2180.981 kb on + strand at 2181.019 kb on + strand at 2181.027 kb on + strand at 2181.037 kb on + strand at 2181.045 kb on - strand at 2181.174 kb on - strand, within rfbD at 2181.179 kb on + strand, within rfbD at 2181.207 kb on - strand, within rfbD at 2181.328 kb on + strand, within rfbD at 2181.342 kb on - strand, within rfbD at 2181.382 kb on + strand, within rfbD at 2181.449 kb on + strand, within rfbD at 2181.553 kb on + strand, within rfbD at 2181.561 kb on - strand, within rfbD at 2181.614 kb on + strand, within rfbD at 2181.622 kb on - strand, within rfbD at 2181.710 kb on + strand, within rfbD at 2181.718 kb on - strand, within rfbD at 2181.727 kb on + strand, within rfbD at 2181.763 kb on + strand, within rfbD at 2181.764 kb on + strand, within rfbD at 2181.773 kb on + strand, within rfbD at 2181.786 kb on - strand, within rfbD at 2181.815 kb on + strand at 2181.823 kb on - strand at 2181.890 kb on - strand at 2181.913 kb on + strand at 2181.921 kb on - strand at 2181.935 kb on - strand at 2182.006 kb on - strand, within rfbB at 2182.007 kb on + strand, within rfbB at 2182.015 kb on - strand, within rfbB at 2182.015 kb on - strand, within rfbB at 2182.015 kb on - strand, within rfbB at 2182.038 kb on - strand, within rfbB at 2182.053 kb on + strand, within rfbB at 2182.093 kb on - strand, within rfbB at 2182.093 kb on - strand, within rfbB at 2182.107 kb on - strand, within rfbB at 2182.152 kb on - strand, within rfbB at 2182.202 kb on - strand, within rfbB at 2182.232 kb on + strand, within rfbB at 2182.258 kb on + strand, within rfbB at 2182.271 kb on - strand, within rfbB at 2182.278 kb on + strand, within rfbB at 2182.279 kb on + strand, within rfbB at 2182.286 kb on - strand, within rfbB at 2182.287 kb on - strand, within rfbB at 2182.362 kb on - strand, within rfbB at 2182.383 kb on + strand, within rfbB at 2182.395 kb on + strand, within rfbB at 2182.403 kb on - strand, within rfbB at 2182.405 kb on - strand, within rfbB at 2182.405 kb on - strand, within rfbB at 2182.457 kb on - strand, within rfbB at 2182.475 kb on - strand, within rfbB at 2182.489 kb on - strand, within rfbB at 2182.566 kb on - strand, within rfbB at 2182.571 kb on + strand, within rfbB at 2182.584 kb on - strand, within rfbB at 2182.584 kb on - strand, within rfbB at 2182.617 kb on - strand, within rfbB at 2182.641 kb on - strand, within rfbB at 2182.762 kb on + strand, within rfbB
Per-strain Table
Position Strand Gene LocusTag Fraction Entrained for one LD cycle; Darkness for 72h then Flask growth in 60 uE constant light remove 2,180,021 + Synpcc7942_2097 0.60 -0.0 2,180,061 + Synpcc7942_2097 0.64 -0.7 2,180,063 + Synpcc7942_2097 0.64 +0.2 2,180,069 - Synpcc7942_2097 0.65 -2.0 2,180,071 + Synpcc7942_2097 0.65 -1.0 2,180,071 + Synpcc7942_2097 0.65 -0.8 2,180,079 - Synpcc7942_2097 0.66 +0.6 2,180,079 - Synpcc7942_2097 0.66 +0.7 2,180,079 - Synpcc7942_2097 0.66 -0.1 2,180,092 + Synpcc7942_2097 0.67 -0.2 2,180,148 - Synpcc7942_2097 0.74 -0.4 2,180,230 + Synpcc7942_2097 0.82 -2.5 2,180,238 - Synpcc7942_2097 0.83 +0.8 2,180,257 - Synpcc7942_2097 0.85 -0.3 2,180,257 - Synpcc7942_2097 0.85 -0.7 2,180,290 + Synpcc7942_2097 0.89 +1.3 2,180,298 - Synpcc7942_2097 0.90 +0.7 2,180,304 - +0.2 2,180,319 + -0.4 2,180,344 - -0.7 2,180,423 - +0.8 2,180,428 - -2.5 2,180,428 - +0.5 2,180,435 - -0.4 2,180,462 - +0.5 2,180,494 - -3.8 2,180,542 + rfbC Synpcc7942_2098 0.15 -2.5 2,180,677 + rfbC Synpcc7942_2098 0.40 +1.0 2,180,685 - rfbC Synpcc7942_2098 0.41 +1.6 2,180,713 + rfbC Synpcc7942_2098 0.46 +0.3 2,180,734 + rfbC Synpcc7942_2098 0.50 +1.0 2,180,754 - rfbC Synpcc7942_2098 0.54 +0.2 2,180,793 + rfbC Synpcc7942_2098 0.61 +2.2 2,180,891 + rfbC Synpcc7942_2098 0.79 +0.7 2,180,899 - rfbC Synpcc7942_2098 0.80 +0.6 2,180,946 + rfbC Synpcc7942_2098 0.89 +0.1 2,180,954 - +0.5 2,180,981 + +0.3 2,181,019 + +1.5 2,181,027 + +1.8 2,181,037 + +1.4 2,181,045 - +3.1 2,181,174 - rfbD Synpcc7942_2099 0.19 +1.9 2,181,179 + rfbD Synpcc7942_2099 0.20 +1.0 2,181,207 - rfbD Synpcc7942_2099 0.23 +0.8 2,181,328 + rfbD Synpcc7942_2099 0.37 +2.8 2,181,342 - rfbD Synpcc7942_2099 0.39 +2.1 2,181,382 + rfbD Synpcc7942_2099 0.43 +1.6 2,181,449 + rfbD Synpcc7942_2099 0.51 +2.4 2,181,553 + rfbD Synpcc7942_2099 0.63 +1.6 2,181,561 - rfbD Synpcc7942_2099 0.64 +2.0 2,181,614 + rfbD Synpcc7942_2099 0.70 +2.7 2,181,622 - rfbD Synpcc7942_2099 0.71 -3.7 2,181,710 + rfbD Synpcc7942_2099 0.81 +2.0 2,181,718 - rfbD Synpcc7942_2099 0.82 +0.2 2,181,727 + rfbD Synpcc7942_2099 0.83 -0.3 2,181,763 + rfbD Synpcc7942_2099 0.87 +1.6 2,181,764 + rfbD Synpcc7942_2099 0.87 +1.2 2,181,773 + rfbD Synpcc7942_2099 0.88 +1.4 2,181,786 - rfbD Synpcc7942_2099 0.90 +2.2 2,181,815 + +2.1 2,181,823 - +2.4 2,181,890 - -1.6 2,181,913 + +0.4 2,181,921 - +0.9 2,181,935 - +0.8 2,182,006 - rfbB Synpcc7942_2100 0.13 +0.5 2,182,007 + rfbB Synpcc7942_2100 0.13 +1.9 2,182,015 - rfbB Synpcc7942_2100 0.13 +0.2 2,182,015 - rfbB Synpcc7942_2100 0.13 -2.1 2,182,015 - rfbB Synpcc7942_2100 0.13 -1.3 2,182,038 - rfbB Synpcc7942_2100 0.16 +0.0 2,182,053 + rfbB Synpcc7942_2100 0.17 +0.1 2,182,093 - rfbB Synpcc7942_2100 0.21 -0.8 2,182,093 - rfbB Synpcc7942_2100 0.21 +1.2 2,182,107 - rfbB Synpcc7942_2100 0.22 +0.2 2,182,152 - rfbB Synpcc7942_2100 0.26 +1.2 2,182,202 - rfbB Synpcc7942_2100 0.31 +0.9 2,182,232 + rfbB Synpcc7942_2100 0.34 +1.0 2,182,258 + rfbB Synpcc7942_2100 0.36 +1.7 2,182,271 - rfbB Synpcc7942_2100 0.37 -2.8 2,182,278 + rfbB Synpcc7942_2100 0.38 +0.3 2,182,279 + rfbB Synpcc7942_2100 0.38 -0.4 2,182,286 - rfbB Synpcc7942_2100 0.39 +0.0 2,182,287 - rfbB Synpcc7942_2100 0.39 +1.1 2,182,362 - rfbB Synpcc7942_2100 0.46 -3.1 2,182,383 + rfbB Synpcc7942_2100 0.48 +1.7 2,182,395 + rfbB Synpcc7942_2100 0.49 -1.2 2,182,403 - rfbB Synpcc7942_2100 0.50 -1.0 2,182,405 - rfbB Synpcc7942_2100 0.50 -0.8 2,182,405 - rfbB Synpcc7942_2100 0.50 -0.5 2,182,457 - rfbB Synpcc7942_2100 0.55 +0.9 2,182,475 - rfbB Synpcc7942_2100 0.56 +0.6 2,182,489 - rfbB Synpcc7942_2100 0.58 +1.3 2,182,566 - rfbB Synpcc7942_2100 0.65 +0.8 2,182,571 + rfbB Synpcc7942_2100 0.65 +0.9 2,182,584 - rfbB Synpcc7942_2100 0.66 +1.9 2,182,584 - rfbB Synpcc7942_2100 0.66 +1.0 2,182,617 - rfbB Synpcc7942_2100 0.69 +0.1 2,182,641 - rfbB Synpcc7942_2100 0.72 -0.2 2,182,762 + rfbB Synpcc7942_2100 0.83 +1.1
Or see this region's nucleotide sequence