Experiment: Entrained for one LD cycle; Darkness for 72h then Flask growth in 60 uE constant light
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ppk and Synpcc7942_1567 are separated by 57 nucleotides Synpcc7942_1567 and Synpcc7942_1568 are separated by 337 nucleotides Synpcc7942_1568 and Synpcc7942_1569 are separated by 57 nucleotides
Synpcc7942_1566: ppk - polyphosphate kinase, at 1,623,449 to 1,625,644
ppk
Synpcc7942_1567: Synpcc7942_1567 - possible ribosomal protein L36, at 1,625,702 to 1,626,019
_1567
Synpcc7942_1568: Synpcc7942_1568 - hypothetical protein, at 1,626,357 to 1,626,596
_1568
Synpcc7942_1569: Synpcc7942_1569 - hypothetical protein, at 1,626,654 to 1,627,115
_1569
Position (kb)
1625
1626
1627 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1624.710 kb on - strand, within ppk at 1624.737 kb on + strand, within ppk at 1624.794 kb on - strand, within ppk at 1624.802 kb on + strand, within ppk at 1624.824 kb on - strand, within ppk at 1624.859 kb on + strand, within ppk at 1624.867 kb on - strand, within ppk at 1624.867 kb on - strand, within ppk at 1624.874 kb on - strand, within ppk at 1624.896 kb on - strand, within ppk at 1624.898 kb on + strand, within ppk at 1624.940 kb on + strand, within ppk at 1624.974 kb on - strand, within ppk at 1624.989 kb on - strand, within ppk at 1624.991 kb on + strand, within ppk at 1624.999 kb on - strand, within ppk at 1625.117 kb on - strand at 1625.229 kb on - strand, within ppk at 1625.238 kb on - strand, within ppk at 1625.240 kb on + strand, within ppk at 1625.286 kb on + strand, within ppk at 1625.286 kb on - strand, within ppk at 1625.319 kb on - strand, within ppk at 1625.428 kb on + strand at 1625.429 kb on - strand at 1625.433 kb on + strand at 1625.434 kb on - strand at 1625.492 kb on - strand at 1625.550 kb on + strand at 1625.553 kb on + strand at 1625.556 kb on - strand at 1625.563 kb on - strand at 1625.602 kb on - strand at 1625.630 kb on + strand at 1625.638 kb on - strand at 1625.651 kb on + strand at 1625.656 kb on + strand at 1625.656 kb on + strand at 1625.664 kb on - strand at 1625.672 kb on - strand at 1625.680 kb on + strand at 1625.771 kb on - strand, within Synpcc7942_1567 at 1625.795 kb on - strand, within Synpcc7942_1567 at 1625.821 kb on + strand, within Synpcc7942_1567 at 1625.825 kb on + strand, within Synpcc7942_1567 at 1625.842 kb on + strand, within Synpcc7942_1567 at 1625.850 kb on - strand, within Synpcc7942_1567 at 1625.850 kb on - strand, within Synpcc7942_1567 at 1625.968 kb on - strand, within Synpcc7942_1567 at 1626.019 kb on - strand at 1626.049 kb on + strand at 1626.091 kb on - strand at 1626.091 kb on - strand at 1626.092 kb on + strand at 1626.092 kb on + strand at 1626.092 kb on + strand at 1626.100 kb on - strand at 1626.100 kb on - strand at 1626.100 kb on - strand at 1626.100 kb on - strand at 1626.135 kb on - strand at 1626.135 kb on - strand at 1626.152 kb on + strand at 1626.160 kb on - strand at 1626.160 kb on - strand at 1626.179 kb on - strand at 1626.204 kb on + strand at 1626.227 kb on - strand at 1626.251 kb on + strand at 1626.251 kb on + strand at 1626.259 kb on - strand at 1626.264 kb on - strand at 1626.276 kb on + strand at 1626.305 kb on + strand at 1626.318 kb on - strand at 1626.323 kb on + strand at 1626.366 kb on - strand at 1626.391 kb on + strand, within Synpcc7942_1568 at 1626.391 kb on + strand, within Synpcc7942_1568 at 1626.394 kb on - strand, within Synpcc7942_1568 at 1626.399 kb on - strand, within Synpcc7942_1568 at 1626.399 kb on - strand, within Synpcc7942_1568 at 1626.403 kb on - strand, within Synpcc7942_1568 at 1626.518 kb on + strand, within Synpcc7942_1568 at 1626.518 kb on + strand, within Synpcc7942_1568 at 1626.518 kb on + strand, within Synpcc7942_1568 at 1626.518 kb on + strand, within Synpcc7942_1568 at 1626.518 kb on + strand, within Synpcc7942_1568 at 1626.518 kb on + strand, within Synpcc7942_1568 at 1626.518 kb on + strand, within Synpcc7942_1568 at 1626.518 kb on + strand, within Synpcc7942_1568 at 1626.518 kb on + strand, within Synpcc7942_1568 at 1626.518 kb on + strand, within Synpcc7942_1568 at 1626.526 kb on - strand, within Synpcc7942_1568 at 1626.526 kb on - strand, within Synpcc7942_1568 at 1626.526 kb on - strand, within Synpcc7942_1568 at 1626.526 kb on - strand, within Synpcc7942_1568 at 1626.526 kb on - strand, within Synpcc7942_1568 at 1626.526 kb on - strand, within Synpcc7942_1568 at 1626.526 kb on - strand, within Synpcc7942_1568 at 1626.526 kb on - strand, within Synpcc7942_1568 at 1626.526 kb on - strand, within Synpcc7942_1568 at 1626.526 kb on - strand, within Synpcc7942_1568 at 1626.526 kb on - strand, within Synpcc7942_1568 at 1626.526 kb on - strand, within Synpcc7942_1568 at 1626.526 kb on - strand, within Synpcc7942_1568 at 1626.607 kb on + strand at 1626.649 kb on + strand at 1626.651 kb on + strand at 1626.657 kb on + strand at 1626.659 kb on - strand at 1626.681 kb on - strand at 1626.708 kb on + strand, within Synpcc7942_1569 at 1626.716 kb on - strand, within Synpcc7942_1569 at 1626.764 kb on - strand, within Synpcc7942_1569 at 1626.774 kb on + strand, within Synpcc7942_1569 at 1626.774 kb on + strand, within Synpcc7942_1569 at 1626.782 kb on - strand, within Synpcc7942_1569 at 1626.782 kb on - strand, within Synpcc7942_1569 at 1626.782 kb on - strand, within Synpcc7942_1569 at 1626.782 kb on - strand, within Synpcc7942_1569 at 1626.784 kb on - strand, within Synpcc7942_1569 at 1626.806 kb on + strand, within Synpcc7942_1569 at 1626.809 kb on + strand, within Synpcc7942_1569 at 1626.913 kb on - strand, within Synpcc7942_1569 at 1626.928 kb on + strand, within Synpcc7942_1569 at 1626.946 kb on + strand, within Synpcc7942_1569 at 1626.953 kb on + strand, within Synpcc7942_1569 at 1626.999 kb on + strand, within Synpcc7942_1569
Per-strain Table
Position Strand Gene LocusTag Fraction Entrained for one LD cycle; Darkness for 72h then Flask growth in 60 uE constant light remove 1,624,710 - ppk Synpcc7942_1566 0.57 -0.8 1,624,737 + ppk Synpcc7942_1566 0.59 +0.5 1,624,794 - ppk Synpcc7942_1566 0.61 +2.4 1,624,802 + ppk Synpcc7942_1566 0.62 +1.7 1,624,824 - ppk Synpcc7942_1566 0.63 +1.5 1,624,859 + ppk Synpcc7942_1566 0.64 +2.0 1,624,867 - ppk Synpcc7942_1566 0.65 +1.3 1,624,867 - ppk Synpcc7942_1566 0.65 +1.8 1,624,874 - ppk Synpcc7942_1566 0.65 +0.6 1,624,896 - ppk Synpcc7942_1566 0.66 +2.4 1,624,898 + ppk Synpcc7942_1566 0.66 +0.6 1,624,940 + ppk Synpcc7942_1566 0.68 +1.1 1,624,974 - ppk Synpcc7942_1566 0.69 +1.8 1,624,989 - ppk Synpcc7942_1566 0.70 -0.7 1,624,991 + ppk Synpcc7942_1566 0.70 +0.3 1,624,999 - ppk Synpcc7942_1566 0.71 +0.0 1,625,117 - +0.7 1,625,229 - ppk Synpcc7942_1566 0.81 +1.8 1,625,238 - ppk Synpcc7942_1566 0.81 +1.3 1,625,240 + ppk Synpcc7942_1566 0.82 -1.8 1,625,286 + ppk Synpcc7942_1566 0.84 +0.8 1,625,286 - ppk Synpcc7942_1566 0.84 +0.5 1,625,319 - ppk Synpcc7942_1566 0.85 +0.6 1,625,428 + +1.5 1,625,429 - +0.8 1,625,433 + +1.3 1,625,434 - +0.2 1,625,492 - -0.1 1,625,550 + -1.0 1,625,553 + +2.8 1,625,556 - -0.5 1,625,563 - +0.2 1,625,602 - -0.6 1,625,630 + +0.9 1,625,638 - -0.4 1,625,651 + -1.4 1,625,656 + -0.1 1,625,656 + +0.4 1,625,664 - +1.6 1,625,672 - -0.6 1,625,680 + +0.6 1,625,771 - Synpcc7942_1567 0.22 +1.0 1,625,795 - Synpcc7942_1567 0.29 -1.7 1,625,821 + Synpcc7942_1567 0.37 -1.3 1,625,825 + Synpcc7942_1567 0.39 -1.6 1,625,842 + Synpcc7942_1567 0.44 -1.3 1,625,850 - Synpcc7942_1567 0.47 -0.7 1,625,850 - Synpcc7942_1567 0.47 -0.3 1,625,968 - Synpcc7942_1567 0.84 -1.3 1,626,019 - +0.5 1,626,049 + +0.9 1,626,091 - -0.8 1,626,091 - -0.8 1,626,092 + +0.4 1,626,092 + +0.2 1,626,092 + -0.5 1,626,100 - -1.2 1,626,100 - -0.5 1,626,100 - +1.0 1,626,100 - -0.6 1,626,135 - +0.8 1,626,135 - +1.1 1,626,152 + -0.8 1,626,160 - -0.7 1,626,160 - -0.6 1,626,179 - -0.6 1,626,204 + -0.8 1,626,227 - -0.8 1,626,251 + -0.5 1,626,251 + +1.8 1,626,259 - +0.2 1,626,264 - -0.5 1,626,276 + +0.7 1,626,305 + -0.4 1,626,318 - -0.4 1,626,323 + +0.5 1,626,366 - -1.4 1,626,391 + Synpcc7942_1568 0.14 +0.5 1,626,391 + Synpcc7942_1568 0.14 -0.8 1,626,394 - Synpcc7942_1568 0.15 -0.5 1,626,399 - Synpcc7942_1568 0.17 +0.6 1,626,399 - Synpcc7942_1568 0.17 -0.3 1,626,403 - Synpcc7942_1568 0.19 -0.4 1,626,518 + Synpcc7942_1568 0.67 -2.1 1,626,518 + Synpcc7942_1568 0.67 +1.4 1,626,518 + Synpcc7942_1568 0.67 -1.0 1,626,518 + Synpcc7942_1568 0.67 +0.6 1,626,518 + Synpcc7942_1568 0.67 -0.9 1,626,518 + Synpcc7942_1568 0.67 +2.0 1,626,518 + Synpcc7942_1568 0.67 -0.4 1,626,518 + Synpcc7942_1568 0.67 -0.0 1,626,518 + Synpcc7942_1568 0.67 +0.5 1,626,518 + Synpcc7942_1568 0.67 -1.2 1,626,526 - Synpcc7942_1568 0.70 +1.3 1,626,526 - Synpcc7942_1568 0.70 -1.0 1,626,526 - Synpcc7942_1568 0.70 +0.1 1,626,526 - Synpcc7942_1568 0.70 -0.4 1,626,526 - Synpcc7942_1568 0.70 +1.1 1,626,526 - Synpcc7942_1568 0.70 +0.4 1,626,526 - Synpcc7942_1568 0.70 -1.8 1,626,526 - Synpcc7942_1568 0.70 -2.1 1,626,526 - Synpcc7942_1568 0.70 -0.0 1,626,526 - Synpcc7942_1568 0.70 -0.4 1,626,526 - Synpcc7942_1568 0.70 -0.1 1,626,526 - Synpcc7942_1568 0.70 +0.5 1,626,526 - Synpcc7942_1568 0.70 -3.3 1,626,607 + +0.6 1,626,649 + -3.6 1,626,651 + +1.2 1,626,657 + +0.2 1,626,659 - -0.2 1,626,681 - +1.1 1,626,708 + Synpcc7942_1569 0.12 -0.3 1,626,716 - Synpcc7942_1569 0.13 +1.1 1,626,764 - Synpcc7942_1569 0.24 +0.3 1,626,774 + Synpcc7942_1569 0.26 -0.0 1,626,774 + Synpcc7942_1569 0.26 -0.4 1,626,782 - Synpcc7942_1569 0.28 +1.7 1,626,782 - Synpcc7942_1569 0.28 +0.6 1,626,782 - Synpcc7942_1569 0.28 +0.4 1,626,782 - Synpcc7942_1569 0.28 +0.4 1,626,784 - Synpcc7942_1569 0.28 -0.1 1,626,806 + Synpcc7942_1569 0.33 -1.2 1,626,809 + Synpcc7942_1569 0.34 -0.5 1,626,913 - Synpcc7942_1569 0.56 +1.0 1,626,928 + Synpcc7942_1569 0.59 +0.7 1,626,946 + Synpcc7942_1569 0.63 +0.6 1,626,953 + Synpcc7942_1569 0.65 -1.4 1,626,999 + Synpcc7942_1569 0.75 -0.5
Or see this region's nucleotide sequence