Experiment: Entrained for one LD cycle; Darkness for 72h then Flask growth in 60 uE constant light
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nadE and nadD overlap by 4 nucleotides nadD and Synpcc7942_0107 are separated by 12 nucleotides Synpcc7942_0107 and Synpcc7942_0108 are separated by 172 nucleotides Synpcc7942_0108 and Synpcc7942_0109 are separated by 60 nucleotides
Synpcc7942_0105: nadE - NAD synthetase, at 104,653 to 106,377
nadE
Synpcc7942_0106: nadD - nicotinic acid mononucleotide adenyltransferase, at 106,374 to 106,952
nadD
Synpcc7942_0107: Synpcc7942_0107 - Small GTP-binding protein domain, at 106,965 to 108,383
_0107
Synpcc7942_0108: Synpcc7942_0108 - sulfiredoxin, at 108,556 to 108,816
_0108
Synpcc7942_0109: Synpcc7942_0109 - DNA-binding ferritin-like protein (oxidative damage protectant)-like, at 108,877 to 109,383
_0109
Position (kb)
106
107
108
109 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 106.379 kb on - strand at 106.396 kb on + strand at 106.396 kb on + strand at 106.396 kb on + strand at 106.404 kb on - strand at 106.410 kb on - strand at 106.763 kb on - strand, within nadD at 106.763 kb on - strand, within nadD at 106.801 kb on - strand, within nadD at 106.829 kb on + strand, within nadD at 106.837 kb on - strand, within nadD at 106.892 kb on - strand, within nadD at 106.935 kb on + strand at 106.954 kb on + strand at 106.955 kb on - strand at 107.004 kb on + strand at 107.030 kb on + strand at 107.038 kb on - strand at 107.040 kb on + strand at 107.079 kb on + strand at 108.344 kb on - strand at 108.344 kb on - strand at 108.346 kb on + strand at 108.346 kb on + strand at 108.365 kb on + strand at 108.408 kb on + strand at 108.411 kb on - strand at 108.411 kb on - strand at 108.436 kb on + strand at 108.436 kb on + strand at 108.436 kb on + strand at 108.441 kb on + strand at 108.444 kb on - strand at 108.444 kb on - strand at 108.444 kb on - strand at 108.444 kb on - strand at 108.444 kb on - strand at 108.444 kb on - strand at 108.444 kb on - strand at 108.449 kb on - strand at 108.449 kb on - strand at 108.452 kb on + strand at 108.452 kb on + strand at 108.460 kb on - strand at 108.460 kb on - strand at 108.462 kb on - strand at 108.463 kb on - strand at 108.463 kb on - strand at 108.465 kb on + strand at 108.473 kb on - strand at 108.473 kb on - strand at 108.473 kb on - strand at 108.571 kb on - strand at 108.571 kb on - strand at 108.573 kb on - strand at 108.578 kb on - strand at 108.603 kb on + strand, within Synpcc7942_0108 at 108.603 kb on - strand, within Synpcc7942_0108 at 108.608 kb on + strand, within Synpcc7942_0108 at 108.611 kb on - strand, within Synpcc7942_0108 at 108.625 kb on + strand, within Synpcc7942_0108 at 108.625 kb on + strand, within Synpcc7942_0108 at 108.638 kb on - strand, within Synpcc7942_0108 at 108.650 kb on + strand, within Synpcc7942_0108 at 108.665 kb on - strand, within Synpcc7942_0108 at 108.672 kb on + strand, within Synpcc7942_0108 at 108.750 kb on - strand, within Synpcc7942_0108 at 108.793 kb on + strand at 108.793 kb on + strand at 108.801 kb on - strand at 108.801 kb on - strand at 108.810 kb on + strand at 108.829 kb on + strand at 108.829 kb on + strand at 108.837 kb on - strand at 108.837 kb on - strand at 108.837 kb on - strand at 108.837 kb on - strand at 108.842 kb on + strand at 108.844 kb on + strand at 108.860 kb on - strand at 108.873 kb on - strand at 108.933 kb on + strand, within Synpcc7942_0109 at 108.953 kb on + strand, within Synpcc7942_0109 at 108.978 kb on + strand, within Synpcc7942_0109 at 108.983 kb on + strand, within Synpcc7942_0109 at 108.987 kb on + strand, within Synpcc7942_0109 at 108.989 kb on + strand, within Synpcc7942_0109 at 108.989 kb on - strand, within Synpcc7942_0109 at 108.995 kb on - strand, within Synpcc7942_0109 at 108.997 kb on - strand, within Synpcc7942_0109 at 109.044 kb on + strand, within Synpcc7942_0109 at 109.045 kb on - strand, within Synpcc7942_0109 at 109.050 kb on - strand, within Synpcc7942_0109 at 109.052 kb on - strand, within Synpcc7942_0109 at 109.059 kb on - strand, within Synpcc7942_0109 at 109.089 kb on + strand, within Synpcc7942_0109 at 109.152 kb on - strand, within Synpcc7942_0109 at 109.158 kb on - strand, within Synpcc7942_0109 at 109.227 kb on - strand at 109.227 kb on - strand, within Synpcc7942_0109 at 109.295 kb on + strand, within Synpcc7942_0109 at 109.303 kb on - strand, within Synpcc7942_0109 at 109.314 kb on + strand, within Synpcc7942_0109 at 109.359 kb on + strand at 109.362 kb on + strand at 109.370 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Entrained for one LD cycle; Darkness for 72h then Flask growth in 60 uE constant light remove 106,379 - +0.7 106,396 + +2.4 106,396 + +0.3 106,396 + +0.6 106,404 - +0.8 106,410 - -0.1 106,763 - nadD Synpcc7942_0106 0.67 +0.3 106,763 - nadD Synpcc7942_0106 0.67 +0.6 106,801 - nadD Synpcc7942_0106 0.74 -0.1 106,829 + nadD Synpcc7942_0106 0.79 +1.3 106,837 - nadD Synpcc7942_0106 0.80 +1.0 106,892 - nadD Synpcc7942_0106 0.89 +0.9 106,935 + +2.0 106,954 + -1.2 106,955 - -2.4 107,004 + -0.7 107,030 + -0.8 107,038 - -0.0 107,040 + -1.3 107,079 + -2.8 108,344 - +1.1 108,344 - -2.7 108,346 + -4.2 108,346 + -0.4 108,365 + +0.3 108,408 + +0.4 108,411 - -1.3 108,411 - -0.1 108,436 + +1.0 108,436 + -0.1 108,436 + -0.6 108,441 + -0.5 108,444 - +0.8 108,444 - -0.4 108,444 - -3.5 108,444 - +0.5 108,444 - -0.5 108,444 - -0.7 108,444 - +0.3 108,449 - +0.7 108,449 - -0.7 108,452 + -0.4 108,452 + +0.6 108,460 - -0.0 108,460 - -0.0 108,462 - +0.1 108,463 - +0.8 108,463 - +0.4 108,465 + -1.2 108,473 - -2.1 108,473 - -0.4 108,473 - +0.2 108,571 - +0.3 108,571 - +0.7 108,573 - -0.8 108,578 - -0.0 108,603 + Synpcc7942_0108 0.18 -0.3 108,603 - Synpcc7942_0108 0.18 +0.5 108,608 + Synpcc7942_0108 0.20 +0.1 108,611 - Synpcc7942_0108 0.21 +1.0 108,625 + Synpcc7942_0108 0.26 -3.1 108,625 + Synpcc7942_0108 0.26 -0.7 108,638 - Synpcc7942_0108 0.31 -0.6 108,650 + Synpcc7942_0108 0.36 +0.2 108,665 - Synpcc7942_0108 0.42 +0.7 108,672 + Synpcc7942_0108 0.44 +1.6 108,750 - Synpcc7942_0108 0.74 -1.0 108,793 + -1.1 108,793 + -1.2 108,801 - +0.3 108,801 - -0.2 108,810 + -0.8 108,829 + -3.2 108,829 + -0.4 108,837 - -0.7 108,837 - -0.4 108,837 - +0.6 108,837 - -0.6 108,842 + +0.1 108,844 + -0.0 108,860 - -1.3 108,873 - -2.2 108,933 + Synpcc7942_0109 0.11 -1.0 108,953 + Synpcc7942_0109 0.15 -0.6 108,978 + Synpcc7942_0109 0.20 +0.3 108,983 + Synpcc7942_0109 0.21 +0.0 108,987 + Synpcc7942_0109 0.22 -0.4 108,989 + Synpcc7942_0109 0.22 -1.3 108,989 - Synpcc7942_0109 0.22 +0.5 108,995 - Synpcc7942_0109 0.23 -0.3 108,997 - Synpcc7942_0109 0.24 -0.1 109,044 + Synpcc7942_0109 0.33 -0.3 109,045 - Synpcc7942_0109 0.33 -1.0 109,050 - Synpcc7942_0109 0.34 +0.3 109,052 - Synpcc7942_0109 0.35 -0.6 109,059 - Synpcc7942_0109 0.36 -0.5 109,089 + Synpcc7942_0109 0.42 -0.1 109,152 - Synpcc7942_0109 0.54 -1.7 109,158 - Synpcc7942_0109 0.55 -1.9 109,227 - -1.9 109,227 - Synpcc7942_0109 0.69 -1.4 109,295 + Synpcc7942_0109 0.82 -2.4 109,303 - Synpcc7942_0109 0.84 +2.3 109,314 + Synpcc7942_0109 0.86 -0.3 109,359 + -1.1 109,362 + -0.6 109,370 - -0.9
Or see this region's nucleotide sequence