Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_401 and Pf6N2E2_402 are separated by 97 nucleotides Pf6N2E2_402 and Pf6N2E2_403 are separated by 39 nucleotides Pf6N2E2_403 and Pf6N2E2_404 are separated by 244 nucleotides
Pf6N2E2_401 - Cyn operon transcriptional activator, at 471,321 to 472,220
_401
Pf6N2E2_402 - Carbonic anhydrase (EC 4.2.1.1), at 472,318 to 472,977
_402
Pf6N2E2_403 - Cyanate hydratase (EC 4.2.1.104), at 473,017 to 473,487
_403
Pf6N2E2_404 - Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit, at 473,732 to 473,854
_404
Position (kb) 473
474 Strain fitness (log2 ratio) -1
0
1
2 at 472.018 kb on + strand, within Pf6N2E2_401 at 472.018 kb on + strand, within Pf6N2E2_401 at 472.018 kb on + strand, within Pf6N2E2_401 at 472.019 kb on - strand, within Pf6N2E2_401 at 472.019 kb on - strand, within Pf6N2E2_401 at 472.019 kb on - strand, within Pf6N2E2_401 at 472.186 kb on + strand at 472.187 kb on - strand at 472.187 kb on - strand at 472.279 kb on + strand at 472.279 kb on + strand at 472.279 kb on + strand at 472.279 kb on + strand at 472.280 kb on - strand at 472.280 kb on - strand at 472.280 kb on - strand at 472.601 kb on + strand, within Pf6N2E2_402 at 472.602 kb on - strand, within Pf6N2E2_402 at 472.751 kb on + strand, within Pf6N2E2_402 at 472.861 kb on + strand, within Pf6N2E2_402 at 472.861 kb on + strand, within Pf6N2E2_402 at 472.862 kb on - strand, within Pf6N2E2_402 at 472.862 kb on - strand, within Pf6N2E2_402 at 472.862 kb on - strand, within Pf6N2E2_402 at 472.862 kb on - strand, within Pf6N2E2_402 at 472.862 kb on - strand, within Pf6N2E2_402 at 472.985 kb on - strand at 473.012 kb on - strand at 473.299 kb on + strand, within Pf6N2E2_403 at 473.299 kb on + strand, within Pf6N2E2_403 at 473.299 kb on + strand, within Pf6N2E2_403 at 473.300 kb on - strand, within Pf6N2E2_403 at 473.300 kb on - strand, within Pf6N2E2_403 at 473.309 kb on - strand, within Pf6N2E2_403 at 473.309 kb on - strand, within Pf6N2E2_403 at 473.327 kb on - strand, within Pf6N2E2_403 at 473.327 kb on - strand, within Pf6N2E2_403 at 473.464 kb on + strand at 473.464 kb on + strand at 473.464 kb on + strand at 473.464 kb on + strand at 473.464 kb on + strand at 473.464 kb on + strand at 473.464 kb on + strand at 473.465 kb on - strand at 473.465 kb on - strand at 473.465 kb on - strand at 473.472 kb on + strand at 473.473 kb on - strand at 473.490 kb on + strand at 473.491 kb on - strand at 473.491 kb on - strand at 473.491 kb on - strand at 473.491 kb on - strand at 473.523 kb on - strand at 473.523 kb on - strand at 473.540 kb on + strand at 473.540 kb on + strand at 473.586 kb on + strand at 473.586 kb on + strand at 473.587 kb on - strand at 473.587 kb on - strand at 473.587 kb on - strand at 473.587 kb on - strand at 473.620 kb on - strand at 473.620 kb on - strand at 473.620 kb on - strand at 473.665 kb on + strand at 473.665 kb on + strand at 473.666 kb on - strand at 473.684 kb on - strand at 473.755 kb on + strand, within Pf6N2E2_404 at 473.756 kb on - strand, within Pf6N2E2_404 at 473.756 kb on - strand, within Pf6N2E2_404 at 473.756 kb on - strand, within Pf6N2E2_404 at 473.766 kb on + strand, within Pf6N2E2_404 at 473.766 kb on + strand, within Pf6N2E2_404 at 473.766 kb on + strand, within Pf6N2E2_404 at 473.767 kb on - strand, within Pf6N2E2_404 at 473.787 kb on - strand, within Pf6N2E2_404 at 473.787 kb on - strand, within Pf6N2E2_404 at 473.823 kb on - strand, within Pf6N2E2_404 at 473.830 kb on + strand, within Pf6N2E2_404 at 473.832 kb on + strand, within Pf6N2E2_404 at 473.832 kb on + strand, within Pf6N2E2_404 at 473.832 kb on + strand, within Pf6N2E2_404 at 473.832 kb on + strand, within Pf6N2E2_404 at 473.832 kb on + strand, within Pf6N2E2_404 at 473.832 kb on + strand, within Pf6N2E2_404 at 473.832 kb on + strand, within Pf6N2E2_404 at 473.832 kb on + strand, within Pf6N2E2_404 at 473.832 kb on + strand, within Pf6N2E2_404 at 473.832 kb on + strand, within Pf6N2E2_404 at 473.832 kb on - strand, within Pf6N2E2_404 at 473.833 kb on - strand, within Pf6N2E2_404 at 473.833 kb on - strand, within Pf6N2E2_404 at 473.833 kb on - strand, within Pf6N2E2_404 at 473.833 kb on - strand, within Pf6N2E2_404 at 473.833 kb on - strand, within Pf6N2E2_404 at 473.836 kb on + strand, within Pf6N2E2_404 at 473.944 kb on + strand at 473.944 kb on + strand at 473.945 kb on - strand at 473.947 kb on + strand at 473.947 kb on + strand at 473.948 kb on - strand at 473.948 kb on - strand at 473.948 kb on - strand at 473.989 kb on - strand at 473.989 kb on - strand at 473.989 kb on - strand at 473.994 kb on + strand at 473.995 kb on - strand at 474.000 kb on - strand at 474.125 kb on - strand at 474.164 kb on + strand at 474.164 kb on + strand at 474.165 kb on - strand at 474.165 kb on - strand at 474.236 kb on + strand at 474.236 kb on + strand at 474.236 kb on + strand at 474.236 kb on + strand at 474.237 kb on - strand at 474.312 kb on + strand at 474.313 kb on - strand at 474.314 kb on + strand at 474.329 kb on + strand at 474.388 kb on + strand at 474.388 kb on + strand at 474.388 kb on + strand at 474.388 kb on + strand at 474.389 kb on - strand at 474.475 kb on + strand at 474.475 kb on + strand at 474.475 kb on + strand at 474.476 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 472,018 + Pf6N2E2_401 0.77 +1.4 472,018 + Pf6N2E2_401 0.77 +0.7 472,018 + Pf6N2E2_401 0.77 +0.5 472,019 - Pf6N2E2_401 0.78 -0.3 472,019 - Pf6N2E2_401 0.78 +0.7 472,019 - Pf6N2E2_401 0.78 +1.1 472,186 + +0.1 472,187 - -0.7 472,187 - +0.9 472,279 + -0.1 472,279 + -0.9 472,279 + +0.0 472,279 + +0.1 472,280 - -0.2 472,280 - +0.7 472,280 - -0.0 472,601 + Pf6N2E2_402 0.43 -0.6 472,602 - Pf6N2E2_402 0.43 +0.5 472,751 + Pf6N2E2_402 0.66 -0.0 472,861 + Pf6N2E2_402 0.82 -0.3 472,861 + Pf6N2E2_402 0.82 -1.2 472,862 - Pf6N2E2_402 0.82 +0.9 472,862 - Pf6N2E2_402 0.82 -0.1 472,862 - Pf6N2E2_402 0.82 -0.3 472,862 - Pf6N2E2_402 0.82 +0.3 472,862 - Pf6N2E2_402 0.82 +0.7 472,985 - +0.3 473,012 - +0.1 473,299 + Pf6N2E2_403 0.60 -0.6 473,299 + Pf6N2E2_403 0.60 +0.4 473,299 + Pf6N2E2_403 0.60 -0.8 473,300 - Pf6N2E2_403 0.60 +0.7 473,300 - Pf6N2E2_403 0.60 -0.4 473,309 - Pf6N2E2_403 0.62 -0.6 473,309 - Pf6N2E2_403 0.62 -0.5 473,327 - Pf6N2E2_403 0.66 -1.1 473,327 - Pf6N2E2_403 0.66 +0.3 473,464 + +0.7 473,464 + +0.2 473,464 + -0.1 473,464 + -0.0 473,464 + -1.3 473,464 + +0.0 473,464 + -0.1 473,465 - -0.8 473,465 - -0.2 473,465 - +0.1 473,472 + +0.5 473,473 - -0.4 473,490 + +0.1 473,491 - -0.1 473,491 - +0.3 473,491 - +0.4 473,491 - -0.2 473,523 - +2.2 473,523 - +0.6 473,540 + -1.1 473,540 + -0.6 473,586 + -0.1 473,586 + +1.1 473,587 - +0.2 473,587 - -0.1 473,587 - +0.6 473,587 - -0.7 473,620 - -0.8 473,620 - +0.3 473,620 - +0.4 473,665 + +0.6 473,665 + -0.2 473,666 - +0.6 473,684 - +0.2 473,755 + Pf6N2E2_404 0.19 +0.9 473,756 - Pf6N2E2_404 0.20 +0.1 473,756 - Pf6N2E2_404 0.20 -0.4 473,756 - Pf6N2E2_404 0.20 +0.5 473,766 + Pf6N2E2_404 0.28 +0.9 473,766 + Pf6N2E2_404 0.28 +0.1 473,766 + Pf6N2E2_404 0.28 -0.1 473,767 - Pf6N2E2_404 0.28 +0.6 473,787 - Pf6N2E2_404 0.45 +1.1 473,787 - Pf6N2E2_404 0.45 +0.4 473,823 - Pf6N2E2_404 0.74 -0.6 473,830 + Pf6N2E2_404 0.80 -1.2 473,832 + Pf6N2E2_404 0.81 -0.3 473,832 + Pf6N2E2_404 0.81 +0.2 473,832 + Pf6N2E2_404 0.81 +0.1 473,832 + Pf6N2E2_404 0.81 +0.0 473,832 + Pf6N2E2_404 0.81 +0.1 473,832 + Pf6N2E2_404 0.81 +1.1 473,832 + Pf6N2E2_404 0.81 -0.4 473,832 + Pf6N2E2_404 0.81 -0.5 473,832 + Pf6N2E2_404 0.81 +1.1 473,832 + Pf6N2E2_404 0.81 +0.0 473,832 - Pf6N2E2_404 0.81 +0.2 473,833 - Pf6N2E2_404 0.82 -0.6 473,833 - Pf6N2E2_404 0.82 -0.4 473,833 - Pf6N2E2_404 0.82 +0.6 473,833 - Pf6N2E2_404 0.82 -0.2 473,833 - Pf6N2E2_404 0.82 +0.9 473,836 + Pf6N2E2_404 0.85 +0.2 473,944 + +0.6 473,944 + +0.1 473,945 - -0.1 473,947 + -0.2 473,947 + +1.1 473,948 - +0.1 473,948 - +0.2 473,948 - -0.7 473,989 - -0.8 473,989 - -0.2 473,989 - -0.5 473,994 + +0.3 473,995 - +0.9 474,000 - -0.4 474,125 - -0.5 474,164 + -0.2 474,164 + +0.3 474,165 - -0.2 474,165 - -0.3 474,236 + +0.1 474,236 + -0.1 474,236 + -0.2 474,236 + +0.7 474,237 - +0.1 474,312 + -0.6 474,313 - +0.2 474,314 + -0.4 474,329 + -0.6 474,388 + +1.3 474,388 + -0.3 474,388 + -0.4 474,388 + -0.7 474,389 - -0.5 474,475 + -0.6 474,475 + -0.1 474,475 + +0.2 474,476 - -0.5
Or see this region's nucleotide sequence