Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03140 and MMJJ_RS03145 are separated by 29 nucleotides MMJJ_RS03145 and MMJJ_RS03150 are separated by 105 nucleotides MMJJ_RS03150 and MMJJ_RS03155 are separated by 19 nucleotides
MMJJ_RS03140: MMJJ_RS03140 - tRNA-dihydrouridine synthase, at 592,913 to 593,683
_RS03140
MMJJ_RS03145: MMJJ_RS03145 - GTP cyclohydrolase IIa, at 593,713 to 594,513
_RS03145
MMJJ_RS03150: MMJJ_RS03150 - 2-phospho-L-lactate transferase, at 594,619 to 595,548
_RS03150
MMJJ_RS03155: MMJJ_RS03155 - hypothetical protein, at 595,568 to 596,653
_RS03155
Position (kb)
594
595
596 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 593.672 kb on - strand at 593.697 kb on - strand at 593.698 kb on + strand at 593.715 kb on + strand at 593.729 kb on - strand at 593.729 kb on - strand at 593.729 kb on - strand at 593.729 kb on - strand at 593.730 kb on + strand at 593.730 kb on + strand at 593.730 kb on + strand at 593.730 kb on + strand at 593.730 kb on + strand at 593.730 kb on + strand at 593.730 kb on + strand at 593.735 kb on - strand at 593.736 kb on + strand at 593.778 kb on + strand at 593.946 kb on - strand at 593.959 kb on + strand, within MMJJ_RS03145 at 594.755 kb on + strand, within MMJJ_RS03150 at 594.763 kb on - strand, within MMJJ_RS03150 at 594.764 kb on + strand, within MMJJ_RS03150 at 594.776 kb on - strand, within MMJJ_RS03150 at 595.044 kb on - strand, within MMJJ_RS03150 at 595.389 kb on + strand, within MMJJ_RS03150 at 595.422 kb on - strand, within MMJJ_RS03150 at 595.432 kb on + strand, within MMJJ_RS03150 at 595.432 kb on + strand, within MMJJ_RS03150 at 595.433 kb on - strand, within MMJJ_RS03150 at 595.433 kb on - strand, within MMJJ_RS03150 at 595.433 kb on - strand, within MMJJ_RS03150 at 595.434 kb on + strand, within MMJJ_RS03150 at 595.434 kb on + strand, within MMJJ_RS03150 at 595.434 kb on + strand, within MMJJ_RS03150 at 595.434 kb on + strand, within MMJJ_RS03150 at 595.434 kb on + strand, within MMJJ_RS03150 at 595.437 kb on - strand, within MMJJ_RS03150 at 595.454 kb on + strand, within MMJJ_RS03150 at 595.563 kb on - strand at 595.563 kb on - strand at 595.595 kb on - strand at 595.596 kb on + strand at 595.596 kb on + strand at 595.596 kb on + strand at 595.600 kb on - strand at 595.601 kb on + strand at 595.618 kb on - strand at 595.624 kb on + strand at 595.656 kb on - strand at 595.711 kb on - strand, within MMJJ_RS03155 at 595.711 kb on - strand, within MMJJ_RS03155 at 595.712 kb on + strand, within MMJJ_RS03155 at 595.712 kb on + strand, within MMJJ_RS03155 at 595.717 kb on + strand, within MMJJ_RS03155 at 595.795 kb on - strand at 595.796 kb on + strand, within MMJJ_RS03155 at 595.799 kb on - strand, within MMJJ_RS03155 at 595.800 kb on + strand, within MMJJ_RS03155 at 595.800 kb on + strand, within MMJJ_RS03155 at 595.803 kb on + strand, within MMJJ_RS03155 at 595.832 kb on - strand, within MMJJ_RS03155 at 595.856 kb on - strand, within MMJJ_RS03155 at 595.859 kb on - strand, within MMJJ_RS03155 at 595.925 kb on + strand, within MMJJ_RS03155 at 595.931 kb on - strand, within MMJJ_RS03155 at 595.952 kb on - strand, within MMJJ_RS03155 at 595.953 kb on + strand, within MMJJ_RS03155 at 595.960 kb on + strand, within MMJJ_RS03155 at 595.960 kb on + strand, within MMJJ_RS03155 at 595.986 kb on + strand, within MMJJ_RS03155 at 596.024 kb on - strand, within MMJJ_RS03155 at 596.029 kb on - strand, within MMJJ_RS03155 at 596.052 kb on + strand, within MMJJ_RS03155 at 596.060 kb on - strand, within MMJJ_RS03155 at 596.065 kb on - strand, within MMJJ_RS03155 at 596.065 kb on - strand, within MMJJ_RS03155 at 596.066 kb on + strand, within MMJJ_RS03155 at 596.067 kb on - strand, within MMJJ_RS03155 at 596.068 kb on + strand, within MMJJ_RS03155 at 596.081 kb on + strand, within MMJJ_RS03155 at 596.153 kb on + strand, within MMJJ_RS03155 at 596.155 kb on + strand, within MMJJ_RS03155 at 596.167 kb on - strand, within MMJJ_RS03155 at 596.192 kb on - strand, within MMJJ_RS03155 at 596.193 kb on + strand, within MMJJ_RS03155 at 596.247 kb on + strand, within MMJJ_RS03155 at 596.247 kb on + strand, within MMJJ_RS03155 at 596.299 kb on - strand, within MMJJ_RS03155 at 596.377 kb on + strand, within MMJJ_RS03155 at 596.384 kb on - strand, within MMJJ_RS03155 at 596.385 kb on + strand, within MMJJ_RS03155 at 596.388 kb on + strand, within MMJJ_RS03155 at 596.439 kb on + strand, within MMJJ_RS03155 at 596.439 kb on + strand, within MMJJ_RS03155 at 596.441 kb on + strand, within MMJJ_RS03155 at 596.536 kb on - strand, within MMJJ_RS03155
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE remove 593,672 - -3.1 593,697 - -1.3 593,698 + -0.6 593,715 + +0.4 593,729 - -0.2 593,729 - +0.5 593,729 - -1.5 593,729 - +0.2 593,730 + -2.3 593,730 + -0.9 593,730 + +0.9 593,730 + -0.4 593,730 + -0.8 593,730 + -0.6 593,730 + +0.5 593,735 - -0.6 593,736 + +0.7 593,778 + -1.4 593,946 - -1.1 593,959 + MMJJ_RS03145 0.31 -2.4 594,755 + MMJJ_RS03150 0.15 -2.0 594,763 - MMJJ_RS03150 0.15 -0.4 594,764 + MMJJ_RS03150 0.16 -2.6 594,776 - MMJJ_RS03150 0.17 -2.4 595,044 - MMJJ_RS03150 0.46 -2.3 595,389 + MMJJ_RS03150 0.83 -2.7 595,422 - MMJJ_RS03150 0.86 -2.3 595,432 + MMJJ_RS03150 0.87 -1.7 595,432 + MMJJ_RS03150 0.87 -2.5 595,433 - MMJJ_RS03150 0.88 -1.4 595,433 - MMJJ_RS03150 0.88 -2.1 595,433 - MMJJ_RS03150 0.88 -3.5 595,434 + MMJJ_RS03150 0.88 -3.1 595,434 + MMJJ_RS03150 0.88 -2.5 595,434 + MMJJ_RS03150 0.88 -1.1 595,434 + MMJJ_RS03150 0.88 -2.7 595,434 + MMJJ_RS03150 0.88 -2.9 595,437 - MMJJ_RS03150 0.88 -2.0 595,454 + MMJJ_RS03150 0.90 -2.8 595,563 - -1.3 595,563 - -0.2 595,595 - -0.8 595,596 + -0.2 595,596 + +0.3 595,596 + +0.2 595,600 - -0.1 595,601 + -0.8 595,618 - +1.5 595,624 + +1.2 595,656 - -0.9 595,711 - MMJJ_RS03155 0.13 -2.4 595,711 - MMJJ_RS03155 0.13 -0.4 595,712 + MMJJ_RS03155 0.13 -2.5 595,712 + MMJJ_RS03155 0.13 -1.6 595,717 + MMJJ_RS03155 0.14 -2.2 595,795 - +0.2 595,796 + MMJJ_RS03155 0.21 -0.7 595,799 - MMJJ_RS03155 0.21 -2.0 595,800 + MMJJ_RS03155 0.21 +1.2 595,800 + MMJJ_RS03155 0.21 -1.2 595,803 + MMJJ_RS03155 0.22 -0.3 595,832 - MMJJ_RS03155 0.24 -1.5 595,856 - MMJJ_RS03155 0.27 -1.1 595,859 - MMJJ_RS03155 0.27 -2.3 595,925 + MMJJ_RS03155 0.33 -2.3 595,931 - MMJJ_RS03155 0.33 -3.5 595,952 - MMJJ_RS03155 0.35 -1.3 595,953 + MMJJ_RS03155 0.35 -1.4 595,960 + MMJJ_RS03155 0.36 -0.3 595,960 + MMJJ_RS03155 0.36 -2.2 595,986 + MMJJ_RS03155 0.38 -1.4 596,024 - MMJJ_RS03155 0.42 -1.5 596,029 - MMJJ_RS03155 0.42 -3.7 596,052 + MMJJ_RS03155 0.45 -1.6 596,060 - MMJJ_RS03155 0.45 -1.8 596,065 - MMJJ_RS03155 0.46 -3.4 596,065 - MMJJ_RS03155 0.46 -1.1 596,066 + MMJJ_RS03155 0.46 -2.0 596,067 - MMJJ_RS03155 0.46 -1.3 596,068 + MMJJ_RS03155 0.46 -1.1 596,081 + MMJJ_RS03155 0.47 -2.1 596,153 + MMJJ_RS03155 0.54 -1.3 596,155 + MMJJ_RS03155 0.54 -2.9 596,167 - MMJJ_RS03155 0.55 -3.5 596,192 - MMJJ_RS03155 0.57 -0.4 596,193 + MMJJ_RS03155 0.58 -1.6 596,247 + MMJJ_RS03155 0.63 +0.6 596,247 + MMJJ_RS03155 0.63 -2.2 596,299 - MMJJ_RS03155 0.67 -1.3 596,377 + MMJJ_RS03155 0.74 -2.2 596,384 - MMJJ_RS03155 0.75 -1.1 596,385 + MMJJ_RS03155 0.75 -0.9 596,388 + MMJJ_RS03155 0.76 +0.4 596,439 + MMJJ_RS03155 0.80 -2.3 596,439 + MMJJ_RS03155 0.80 +0.2 596,441 + MMJJ_RS03155 0.80 -1.1 596,536 - MMJJ_RS03155 0.89 -1.0
Or see this region's nucleotide sequence