Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01600 and MMJJ_RS01605 are separated by 101 nucleotides MMJJ_RS01605 and MMJJ_RS01610 are separated by 75 nucleotides MMJJ_RS01610 and MMJJ_RS01615 are separated by 75 nucleotides MMJJ_RS01615 and MMJJ_RS01620 are separated by 57 nucleotides
MMJJ_RS01600: MMJJ_RS01600 - radical SAM protein, at 311,398 to 312,291
_RS01600
MMJJ_RS01605: MMJJ_RS01605 - ACT domain-containing protein, at 312,393 to 312,665
_RS01605
MMJJ_RS01610: MMJJ_RS01610 - methanogenesis marker 17 protein, at 312,741 to 313,292
_RS01610
MMJJ_RS01615: MMJJ_RS01615 - MoaD/ThiS family protein, at 313,368 to 313,562
_RS01615
MMJJ_RS01620: MMJJ_RS01620 - ketol-acid reductoisomerase, at 313,620 to 314,612
_RS01620
Position (kb) 312
313
314 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 311.775 kb on + strand, within MMJJ_RS01600 at 311.885 kb on + strand, within MMJJ_RS01600 at 311.997 kb on + strand, within MMJJ_RS01600 at 312.094 kb on - strand, within MMJJ_RS01600 at 312.134 kb on - strand, within MMJJ_RS01600 at 312.144 kb on + strand at 312.144 kb on + strand, within MMJJ_RS01600 at 312.216 kb on - strand at 312.217 kb on + strand at 312.249 kb on - strand at 312.265 kb on - strand at 312.554 kb on + strand, within MMJJ_RS01605 at 312.615 kb on + strand, within MMJJ_RS01605 at 312.687 kb on + strand at 312.726 kb on + strand at 313.153 kb on - strand, within MMJJ_RS01610 at 313.193 kb on - strand, within MMJJ_RS01610 at 313.268 kb on - strand at 313.285 kb on - strand at 313.334 kb on - strand at 313.337 kb on - strand at 313.337 kb on - strand at 313.337 kb on - strand at 313.339 kb on - strand at 313.353 kb on - strand at 313.357 kb on - strand at 313.358 kb on + strand at 313.358 kb on + strand at 313.399 kb on + strand, within MMJJ_RS01615 at 313.470 kb on - strand, within MMJJ_RS01615 at 313.470 kb on - strand, within MMJJ_RS01615 at 313.470 kb on - strand, within MMJJ_RS01615 at 313.471 kb on + strand, within MMJJ_RS01615 at 313.471 kb on + strand, within MMJJ_RS01615 at 313.471 kb on + strand, within MMJJ_RS01615 at 313.477 kb on + strand, within MMJJ_RS01615 at 313.477 kb on + strand, within MMJJ_RS01615 at 313.548 kb on - strand at 313.599 kb on - strand at 313.613 kb on + strand at 313.617 kb on + strand at 313.622 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE remove 311,775 + MMJJ_RS01600 0.42 -1.7 311,885 + MMJJ_RS01600 0.54 -1.9 311,997 + MMJJ_RS01600 0.67 -2.2 312,094 - MMJJ_RS01600 0.78 -3.2 312,134 - MMJJ_RS01600 0.82 -1.4 312,144 + +1.1 312,144 + MMJJ_RS01600 0.83 -1.7 312,216 - -0.4 312,217 + -0.8 312,249 - -1.9 312,265 - -0.3 312,554 + MMJJ_RS01605 0.59 -1.2 312,615 + MMJJ_RS01605 0.81 -1.4 312,687 + +0.1 312,726 + -0.3 313,153 - MMJJ_RS01610 0.75 -1.7 313,193 - MMJJ_RS01610 0.82 -1.5 313,268 - -1.2 313,285 - -1.5 313,334 - -3.6 313,337 - -1.9 313,337 - -3.3 313,337 - -1.9 313,339 - -1.6 313,353 - -1.7 313,357 - -0.0 313,358 + +0.0 313,358 + +0.2 313,399 + MMJJ_RS01615 0.16 +2.1 313,470 - MMJJ_RS01615 0.52 -0.3 313,470 - MMJJ_RS01615 0.52 -0.7 313,470 - MMJJ_RS01615 0.52 -0.2 313,471 + MMJJ_RS01615 0.53 +0.5 313,471 + MMJJ_RS01615 0.53 +1.8 313,471 + MMJJ_RS01615 0.53 -0.2 313,477 + MMJJ_RS01615 0.56 -0.1 313,477 + MMJJ_RS01615 0.56 +0.1 313,548 - -0.1 313,599 - -0.0 313,613 + -0.0 313,617 + -0.2 313,622 + -0.1
Or see this region's nucleotide sequence