Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trmB and Shewana3_1183 are separated by 45 nucleotides Shewana3_1183 and Shewana3_1184 overlap by 1 nucleotides Shewana3_1184 and Shewana3_1185 are separated by 178 nucleotides
Shewana3_1182: trmB - tRNA (guanine-N(7)-)-methyltransferase (RefSeq), at 1,389,839 to 1,390,555
trmB
Shewana3_1183: Shewana3_1183 - hypothetical protein (RefSeq), at 1,390,601 to 1,390,927
_1183
Shewana3_1184: Shewana3_1184 - Glutaminase (EC 3.5.1.2) (from data) , at 1,390,927 to 1,391,841
_1184
Shewana3_1185: Shewana3_1185 - hypothetical protein (RefSeq), at 1,392,020 to 1,392,841
_1185
Position (kb)
1390
1391
1392 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 1389.960 kb on + strand, within trmB at 1390.008 kb on + strand, within trmB at 1391.340 kb on + strand, within Shewana3_1184 at 1391.348 kb on - strand, within Shewana3_1184 at 1391.401 kb on + strand, within Shewana3_1184 at 1391.409 kb on + strand, within Shewana3_1184 at 1391.482 kb on - strand, within Shewana3_1184 at 1391.547 kb on + strand, within Shewana3_1184 at 1391.598 kb on + strand, within Shewana3_1184 at 1391.731 kb on - strand, within Shewana3_1184 at 1391.744 kb on + strand, within Shewana3_1184 at 1391.752 kb on - strand at 1391.961 kb on - strand at 1392.009 kb on - strand at 1392.035 kb on + strand at 1392.197 kb on + strand, within Shewana3_1185 at 1392.238 kb on - strand, within Shewana3_1185 at 1392.352 kb on + strand, within Shewana3_1185 at 1392.464 kb on - strand, within Shewana3_1185 at 1392.626 kb on + strand, within Shewana3_1185
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 1,389,960 + trmB Shewana3_1182 0.17 -1.7 1,390,008 + trmB Shewana3_1182 0.24 -0.6 1,391,340 + Shewana3_1184 0.45 -5.5 1,391,348 - Shewana3_1184 0.46 -4.6 1,391,401 + Shewana3_1184 0.52 +0.1 1,391,409 + Shewana3_1184 0.53 -5.4 1,391,482 - Shewana3_1184 0.61 -1.9 1,391,547 + Shewana3_1184 0.68 -6.2 1,391,598 + Shewana3_1184 0.73 -3.6 1,391,731 - Shewana3_1184 0.88 -3.3 1,391,744 + Shewana3_1184 0.89 -5.2 1,391,752 - -5.1 1,391,961 - +0.1 1,392,009 - +0.1 1,392,035 + +0.6 1,392,197 + Shewana3_1185 0.22 +0.3 1,392,238 - Shewana3_1185 0.27 +0.1 1,392,352 + Shewana3_1185 0.40 -0.1 1,392,464 - Shewana3_1185 0.54 +0.1 1,392,626 + Shewana3_1185 0.74 -0.4
Or see this region's nucleotide sequence