Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1138 and thrA are separated by 642 nucleotides thrA and Shewana3_1140 overlap by 4 nucleotides
Shewana3_1138: Shewana3_1138 - peptidylprolyl isomerase, FKBP-type (RefSeq), at 1,341,513 to 1,342,181
_1138
Shewana3_1139: thrA - bifunctional aspartokinase I/homeserine dehydrogenase I (RefSeq), at 1,342,824 to 1,345,292
thrA
Shewana3_1140: Shewana3_1140 - homoserine kinase (RefSeq), at 1,345,289 to 1,346,227
_1140
Position (kb)
1342
1343
1344
1345
1346 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 1341.863 kb on - strand, within Shewana3_1138 at 1341.949 kb on + strand, within Shewana3_1138 at 1341.957 kb on - strand, within Shewana3_1138 at 1341.983 kb on - strand, within Shewana3_1138 at 1342.164 kb on + strand at 1342.164 kb on + strand at 1342.254 kb on + strand at 1342.254 kb on + strand at 1342.342 kb on + strand at 1342.371 kb on - strand at 1342.664 kb on - strand at 1342.733 kb on - strand at 1343.146 kb on + strand, within thrA at 1343.146 kb on + strand, within thrA at 1343.146 kb on + strand, within thrA at 1343.154 kb on - strand, within thrA at 1343.428 kb on - strand, within thrA at 1343.436 kb on - strand, within thrA at 1343.520 kb on + strand, within thrA at 1343.536 kb on + strand, within thrA at 1343.597 kb on + strand, within thrA at 1343.608 kb on + strand, within thrA at 1343.616 kb on - strand, within thrA at 1343.616 kb on - strand, within thrA at 1343.632 kb on + strand, within thrA at 1343.696 kb on + strand, within thrA at 1344.049 kb on + strand, within thrA at 1344.578 kb on + strand, within thrA at 1344.592 kb on + strand, within thrA at 1344.598 kb on - strand, within thrA at 1344.773 kb on + strand, within thrA at 1344.781 kb on - strand, within thrA at 1344.785 kb on + strand, within thrA at 1344.853 kb on + strand, within thrA at 1345.043 kb on + strand, within thrA at 1345.070 kb on + strand at 1345.132 kb on + strand at 1345.293 kb on - strand at 1345.820 kb on - strand, within Shewana3_1140 at 1345.928 kb on + strand, within Shewana3_1140 at 1345.935 kb on + strand, within Shewana3_1140 at 1345.943 kb on - strand, within Shewana3_1140 at 1345.970 kb on + strand, within Shewana3_1140 at 1345.972 kb on - strand, within Shewana3_1140 at 1345.978 kb on - strand, within Shewana3_1140 at 1345.995 kb on - strand, within Shewana3_1140 at 1346.215 kb on - strand at 1346.252 kb on + strand at 1346.281 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 1,341,863 - Shewana3_1138 0.52 -1.9 1,341,949 + Shewana3_1138 0.65 +0.5 1,341,957 - Shewana3_1138 0.66 +0.3 1,341,983 - Shewana3_1138 0.70 +0.4 1,342,164 + -1.6 1,342,164 + +0.1 1,342,254 + -2.2 1,342,254 + +0.0 1,342,342 + -1.2 1,342,371 - +0.6 1,342,664 - -4.8 1,342,733 - -5.6 1,343,146 + thrA Shewana3_1139 0.13 -5.0 1,343,146 + thrA Shewana3_1139 0.13 -2.5 1,343,146 + thrA Shewana3_1139 0.13 -5.4 1,343,154 - thrA Shewana3_1139 0.13 -4.9 1,343,428 - thrA Shewana3_1139 0.24 -2.9 1,343,436 - thrA Shewana3_1139 0.25 -5.7 1,343,520 + thrA Shewana3_1139 0.28 -7.4 1,343,536 + thrA Shewana3_1139 0.29 -5.5 1,343,597 + thrA Shewana3_1139 0.31 -3.1 1,343,608 + thrA Shewana3_1139 0.32 -4.0 1,343,616 - thrA Shewana3_1139 0.32 -4.2 1,343,616 - thrA Shewana3_1139 0.32 -4.0 1,343,632 + thrA Shewana3_1139 0.33 -2.2 1,343,696 + thrA Shewana3_1139 0.35 -5.9 1,344,049 + thrA Shewana3_1139 0.50 -2.8 1,344,578 + thrA Shewana3_1139 0.71 -5.4 1,344,592 + thrA Shewana3_1139 0.72 -4.2 1,344,598 - thrA Shewana3_1139 0.72 -4.6 1,344,773 + thrA Shewana3_1139 0.79 -3.3 1,344,781 - thrA Shewana3_1139 0.79 -1.9 1,344,785 + thrA Shewana3_1139 0.79 -3.1 1,344,853 + thrA Shewana3_1139 0.82 -4.5 1,345,043 + thrA Shewana3_1139 0.90 -3.5 1,345,070 + -2.8 1,345,132 + -5.1 1,345,293 - -4.1 1,345,820 - Shewana3_1140 0.57 -5.2 1,345,928 + Shewana3_1140 0.68 -1.9 1,345,935 + Shewana3_1140 0.69 -4.5 1,345,943 - Shewana3_1140 0.70 -4.9 1,345,970 + Shewana3_1140 0.73 -5.4 1,345,972 - Shewana3_1140 0.73 -5.8 1,345,978 - Shewana3_1140 0.73 -6.5 1,345,995 - Shewana3_1140 0.75 -2.1 1,346,215 - -6.5 1,346,252 + -3.1 1,346,281 + -6.5
Or see this region's nucleotide sequence