Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rbn and Shewana3_0312 are separated by 96 nucleotides Shewana3_0312 and Shewana3_0313 overlap by 4 nucleotides Shewana3_0313 and Shewana3_0314 are separated by 15 nucleotides
Shewana3_0311: rbn - ribonuclease BN (RefSeq), at 349,274 to 350,287
rbn
Shewana3_0312: Shewana3_0312 - prolyl aminopeptidase (RefSeq), at 350,384 to 351,289
_0312
Shewana3_0313: Shewana3_0313 - hypothetical protein (RefSeq), at 351,286 to 351,888
_0313
Shewana3_0314: Shewana3_0314 - D-tyrosyl-tRNA(Tyr) deacylase (RefSeq), at 351,904 to 352,341
_0314
Position (kb) 350
351
352 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 349.406 kb on + strand, within rbn at 349.406 kb on + strand, within rbn at 349.414 kb on - strand, within rbn at 349.427 kb on - strand, within rbn at 349.543 kb on - strand, within rbn at 349.543 kb on - strand, within rbn at 349.781 kb on - strand, within rbn at 349.867 kb on + strand, within rbn at 349.942 kb on - strand, within rbn at 349.970 kb on - strand, within rbn at 350.033 kb on - strand, within rbn at 350.100 kb on - strand, within rbn at 350.173 kb on + strand, within rbn at 350.173 kb on + strand, within rbn at 350.384 kb on + strand at 350.444 kb on - strand at 350.444 kb on - strand at 350.675 kb on + strand, within Shewana3_0312 at 350.721 kb on + strand, within Shewana3_0312 at 350.933 kb on + strand, within Shewana3_0312 at 350.983 kb on + strand, within Shewana3_0312 at 351.436 kb on + strand, within Shewana3_0313 at 351.482 kb on - strand, within Shewana3_0313 at 351.529 kb on - strand, within Shewana3_0313 at 351.575 kb on - strand, within Shewana3_0313 at 351.787 kb on - strand, within Shewana3_0313 at 351.844 kb on - strand at 352.055 kb on + strand, within Shewana3_0314 at 352.191 kb on + strand, within Shewana3_0314
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 349,406 + rbn Shewana3_0311 0.13 -0.4 349,406 + rbn Shewana3_0311 0.13 +0.0 349,414 - rbn Shewana3_0311 0.14 -0.3 349,427 - rbn Shewana3_0311 0.15 +0.2 349,543 - rbn Shewana3_0311 0.27 -0.4 349,543 - rbn Shewana3_0311 0.27 +1.0 349,781 - rbn Shewana3_0311 0.50 -0.2 349,867 + rbn Shewana3_0311 0.58 -2.2 349,942 - rbn Shewana3_0311 0.66 -0.2 349,970 - rbn Shewana3_0311 0.69 -0.4 350,033 - rbn Shewana3_0311 0.75 +0.4 350,100 - rbn Shewana3_0311 0.81 -0.1 350,173 + rbn Shewana3_0311 0.89 -1.7 350,173 + rbn Shewana3_0311 0.89 +0.9 350,384 + +0.0 350,444 - -1.6 350,444 - -2.4 350,675 + Shewana3_0312 0.32 -2.5 350,721 + Shewana3_0312 0.37 +0.1 350,933 + Shewana3_0312 0.61 -1.1 350,983 + Shewana3_0312 0.66 -1.1 351,436 + Shewana3_0313 0.25 +0.2 351,482 - Shewana3_0313 0.33 -0.0 351,529 - Shewana3_0313 0.40 +0.3 351,575 - Shewana3_0313 0.48 -0.0 351,787 - Shewana3_0313 0.83 -4.1 351,844 - +0.8 352,055 + Shewana3_0314 0.34 +0.3 352,191 + Shewana3_0314 0.66 -1.8
Or see this region's nucleotide sequence