Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0034 and def are separated by 15 nucleotides def and Shewana3_0036 are separated by 125 nucleotides Shewana3_0036 and Shewana3_0037 are separated by 83 nucleotides
Shewana3_0034: Shewana3_0034 - methionyl-tRNA formyltransferase (RefSeq), at 41,372 to 42,328
_0034
Shewana3_0035: def - peptide deformylase (RefSeq), at 42,344 to 42,850
def
Shewana3_0036: Shewana3_0036 - peptidoglycan-binding LysM (RefSeq), at 42,976 to 44,100
_0036
Shewana3_0037: Shewana3_0037 - DNA protecting protein DprA (RefSeq), at 44,184 to 45,200
_0037
Position (kb) 42
43
44
45 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 42.520 kb on - strand, within def at 42.527 kb on - strand, within def at 42.637 kb on - strand, within def at 42.774 kb on + strand, within def at 43.247 kb on - strand, within Shewana3_0036 at 43.247 kb on - strand, within Shewana3_0036 at 43.247 kb on - strand, within Shewana3_0036 at 43.247 kb on - strand, within Shewana3_0036 at 43.247 kb on - strand, within Shewana3_0036 at 43.292 kb on + strand, within Shewana3_0036 at 43.292 kb on + strand, within Shewana3_0036 at 43.300 kb on - strand, within Shewana3_0036 at 43.300 kb on - strand, within Shewana3_0036 at 43.302 kb on - strand, within Shewana3_0036 at 43.359 kb on - strand, within Shewana3_0036 at 43.366 kb on + strand, within Shewana3_0036 at 43.439 kb on - strand, within Shewana3_0036 at 43.485 kb on - strand, within Shewana3_0036 at 43.517 kb on + strand, within Shewana3_0036 at 43.543 kb on + strand, within Shewana3_0036 at 43.543 kb on + strand, within Shewana3_0036 at 43.681 kb on + strand, within Shewana3_0036 at 43.719 kb on + strand, within Shewana3_0036 at 43.719 kb on + strand, within Shewana3_0036 at 43.719 kb on + strand, within Shewana3_0036 at 43.719 kb on + strand, within Shewana3_0036 at 43.719 kb on + strand, within Shewana3_0036 at 43.722 kb on + strand, within Shewana3_0036 at 43.727 kb on - strand, within Shewana3_0036 at 43.727 kb on - strand, within Shewana3_0036 at 43.727 kb on - strand, within Shewana3_0036 at 43.727 kb on - strand, within Shewana3_0036 at 43.727 kb on - strand, within Shewana3_0036 at 43.727 kb on - strand, within Shewana3_0036 at 43.727 kb on - strand, within Shewana3_0036 at 43.727 kb on - strand, within Shewana3_0036 at 43.727 kb on - strand, within Shewana3_0036 at 43.727 kb on - strand, within Shewana3_0036 at 43.809 kb on + strand, within Shewana3_0036 at 43.817 kb on - strand, within Shewana3_0036 at 43.884 kb on + strand, within Shewana3_0036 at 43.884 kb on + strand, within Shewana3_0036 at 43.892 kb on - strand, within Shewana3_0036 at 43.892 kb on - strand, within Shewana3_0036 at 43.909 kb on + strand, within Shewana3_0036 at 43.945 kb on - strand, within Shewana3_0036 at 43.981 kb on + strand, within Shewana3_0036 at 44.047 kb on - strand at 44.078 kb on + strand at 44.093 kb on - strand at 44.183 kb on + strand at 44.187 kb on + strand at 44.258 kb on - strand at 44.261 kb on - strand at 44.319 kb on - strand, within Shewana3_0037 at 44.348 kb on + strand, within Shewana3_0037 at 44.356 kb on - strand, within Shewana3_0037 at 44.410 kb on + strand, within Shewana3_0037 at 44.452 kb on + strand, within Shewana3_0037 at 44.496 kb on + strand, within Shewana3_0037 at 44.504 kb on - strand, within Shewana3_0037 at 44.573 kb on - strand, within Shewana3_0037 at 44.583 kb on - strand, within Shewana3_0037 at 44.614 kb on - strand, within Shewana3_0037 at 44.777 kb on + strand, within Shewana3_0037 at 44.812 kb on + strand, within Shewana3_0037 at 44.919 kb on + strand, within Shewana3_0037 at 44.937 kb on - strand, within Shewana3_0037 at 45.006 kb on - strand, within Shewana3_0037
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 42,520 - def Shewana3_0035 0.35 +0.6 42,527 - def Shewana3_0035 0.36 +0.1 42,637 - def Shewana3_0035 0.58 +0.4 42,774 + def Shewana3_0035 0.85 +0.7 43,247 - Shewana3_0036 0.24 +0.5 43,247 - Shewana3_0036 0.24 -1.5 43,247 - Shewana3_0036 0.24 +1.5 43,247 - Shewana3_0036 0.24 +0.1 43,247 - Shewana3_0036 0.24 +1.2 43,292 + Shewana3_0036 0.28 +0.9 43,292 + Shewana3_0036 0.28 -3.3 43,300 - Shewana3_0036 0.29 -1.5 43,300 - Shewana3_0036 0.29 -1.4 43,302 - Shewana3_0036 0.29 +0.7 43,359 - Shewana3_0036 0.34 +0.6 43,366 + Shewana3_0036 0.35 -1.5 43,439 - Shewana3_0036 0.41 -0.0 43,485 - Shewana3_0036 0.45 -1.3 43,517 + Shewana3_0036 0.48 +0.1 43,543 + Shewana3_0036 0.50 +0.4 43,543 + Shewana3_0036 0.50 +0.3 43,681 + Shewana3_0036 0.63 +0.3 43,719 + Shewana3_0036 0.66 +0.6 43,719 + Shewana3_0036 0.66 +0.0 43,719 + Shewana3_0036 0.66 -0.1 43,719 + Shewana3_0036 0.66 -1.7 43,719 + Shewana3_0036 0.66 -1.2 43,722 + Shewana3_0036 0.66 +0.2 43,727 - Shewana3_0036 0.67 +0.2 43,727 - Shewana3_0036 0.67 -1.8 43,727 - Shewana3_0036 0.67 +0.8 43,727 - Shewana3_0036 0.67 -1.4 43,727 - Shewana3_0036 0.67 +0.5 43,727 - Shewana3_0036 0.67 +0.1 43,727 - Shewana3_0036 0.67 +0.2 43,727 - Shewana3_0036 0.67 -1.0 43,727 - Shewana3_0036 0.67 -0.0 43,727 - Shewana3_0036 0.67 -2.3 43,809 + Shewana3_0036 0.74 -1.6 43,817 - Shewana3_0036 0.75 +0.4 43,884 + Shewana3_0036 0.81 -2.1 43,884 + Shewana3_0036 0.81 -0.9 43,892 - Shewana3_0036 0.81 -2.0 43,892 - Shewana3_0036 0.81 -0.1 43,909 + Shewana3_0036 0.83 +0.4 43,945 - Shewana3_0036 0.86 +0.3 43,981 + Shewana3_0036 0.89 -1.9 44,047 - -0.6 44,078 + -1.1 44,093 - -1.2 44,183 + -1.8 44,187 + +0.3 44,258 - +0.2 44,261 - -1.3 44,319 - Shewana3_0037 0.13 +0.1 44,348 + Shewana3_0037 0.16 -2.3 44,356 - Shewana3_0037 0.17 +0.9 44,410 + Shewana3_0037 0.22 -0.1 44,452 + Shewana3_0037 0.26 +0.3 44,496 + Shewana3_0037 0.31 -0.0 44,504 - Shewana3_0037 0.31 -1.5 44,573 - Shewana3_0037 0.38 +0.1 44,583 - Shewana3_0037 0.39 +0.2 44,614 - Shewana3_0037 0.42 -0.1 44,777 + Shewana3_0037 0.58 +0.2 44,812 + Shewana3_0037 0.62 +1.7 44,919 + Shewana3_0037 0.72 -1.3 44,937 - Shewana3_0037 0.74 +0.8 45,006 - Shewana3_0037 0.81 +1.1
Or see this region's nucleotide sequence