Experiment: Darkness for 120h then Flask growth in 60 uE constant light
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_B2615 and parB are separated by 102 nucleotides parB and parA overlap by 4 nucleotides parA and sepA2 are separated by 133 nucleotides
Synpcc7942_B2615: Synpcc7942_B2615 - hypothetical protein, at 65 to 394
_B2615
Synpcc7942_B2626: parB - hypothetical protein, at 497 to 1,321
parB
Synpcc7942_B2637: parA - ParA-like protein, at 1,318 to 1,929
parA
Synpcc7942_B2648: sepA2 - hypothetical protein, at 2,063 to 2,329
sepA2
Position (kb) 0
1
2 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 0.018 kb on + strand at 0.042 kb on + strand at 0.051 kb on - strand at 0.052 kb on + strand at 0.053 kb on + strand at 0.060 kb on - strand at 0.060 kb on - strand at 0.070 kb on - strand at 0.070 kb on - strand at 0.071 kb on - strand at 0.072 kb on + strand at 0.077 kb on + strand at 0.085 kb on - strand at 0.088 kb on + strand at 0.096 kb on - strand at 0.133 kb on + strand, within Synpcc7942_B2615 at 0.133 kb on + strand, within Synpcc7942_B2615 at 0.133 kb on + strand, within Synpcc7942_B2615 at 0.133 kb on + strand, within Synpcc7942_B2615 at 0.141 kb on - strand, within Synpcc7942_B2615 at 0.141 kb on - strand, within Synpcc7942_B2615 at 0.143 kb on + strand, within Synpcc7942_B2615 at 0.143 kb on + strand, within Synpcc7942_B2615 at 0.143 kb on + strand, within Synpcc7942_B2615 at 0.151 kb on - strand, within Synpcc7942_B2615 at 0.151 kb on - strand, within Synpcc7942_B2615 at 0.151 kb on - strand, within Synpcc7942_B2615 at 0.151 kb on - strand, within Synpcc7942_B2615 at 0.151 kb on - strand, within Synpcc7942_B2615 at 0.158 kb on + strand, within Synpcc7942_B2615 at 0.158 kb on + strand, within Synpcc7942_B2615 at 0.166 kb on - strand, within Synpcc7942_B2615 at 0.207 kb on - strand, within Synpcc7942_B2615 at 0.207 kb on - strand, within Synpcc7942_B2615 at 0.214 kb on + strand, within Synpcc7942_B2615 at 0.221 kb on + strand, within Synpcc7942_B2615 at 0.221 kb on + strand, within Synpcc7942_B2615 at 0.221 kb on + strand, within Synpcc7942_B2615 at 0.229 kb on - strand, within Synpcc7942_B2615 at 0.229 kb on - strand at 0.229 kb on - strand, within Synpcc7942_B2615 at 0.229 kb on - strand, within Synpcc7942_B2615 at 0.229 kb on - strand, within Synpcc7942_B2615 at 0.229 kb on - strand, within Synpcc7942_B2615 at 0.240 kb on + strand, within Synpcc7942_B2615 at 0.247 kb on + strand, within Synpcc7942_B2615 at 0.247 kb on + strand, within Synpcc7942_B2615 at 0.248 kb on - strand, within Synpcc7942_B2615 at 0.255 kb on - strand, within Synpcc7942_B2615 at 0.255 kb on - strand, within Synpcc7942_B2615 at 0.255 kb on - strand, within Synpcc7942_B2615 at 0.255 kb on - strand, within Synpcc7942_B2615 at 0.255 kb on - strand, within Synpcc7942_B2615 at 0.257 kb on + strand, within Synpcc7942_B2615 at 0.257 kb on + strand, within Synpcc7942_B2615 at 0.257 kb on + strand, within Synpcc7942_B2615 at 0.304 kb on - strand, within Synpcc7942_B2615 at 0.322 kb on + strand, within Synpcc7942_B2615 at 0.360 kb on + strand, within Synpcc7942_B2615 at 0.360 kb on + strand, within Synpcc7942_B2615 at 0.360 kb on + strand, within Synpcc7942_B2615 at 0.373 kb on + strand at 0.373 kb on + strand at 0.373 kb on + strand at 0.373 kb on + strand at 0.373 kb on + strand at 0.373 kb on + strand at 0.373 kb on + strand at 0.373 kb on + strand at 0.373 kb on + strand at 0.373 kb on + strand at 0.381 kb on - strand at 0.381 kb on - strand at 0.381 kb on - strand at 0.381 kb on - strand at 0.381 kb on - strand at 0.381 kb on - strand at 0.396 kb on + strand at 0.401 kb on + strand at 0.401 kb on + strand at 0.401 kb on + strand at 0.401 kb on + strand at 0.401 kb on + strand at 0.404 kb on - strand at 0.404 kb on - strand at 0.407 kb on - strand at 0.409 kb on - strand at 0.409 kb on - strand at 0.409 kb on - strand at 0.414 kb on - strand at 0.414 kb on - strand at 0.414 kb on - strand at 0.431 kb on + strand at 0.495 kb on + strand at 0.495 kb on + strand at 0.495 kb on + strand at 0.495 kb on + strand at 0.510 kb on + strand at 0.512 kb on + strand at 0.514 kb on + strand at 0.565 kb on - strand at 0.601 kb on - strand, within parB at 0.651 kb on + strand, within parB at 0.680 kb on + strand, within parB at 0.748 kb on + strand, within parB at 0.756 kb on - strand, within parB at 0.855 kb on + strand, within parB at 0.877 kb on - strand, within parB at 1.007 kb on + strand, within parB at 1.192 kb on - strand, within parB at 1.218 kb on + strand, within parB at 1.293 kb on - strand at 1.293 kb on - strand at 1.295 kb on + strand at 1.303 kb on - strand at 1.340 kb on - strand at 1.362 kb on - strand at 1.380 kb on + strand, within parA at 1.380 kb on + strand, within parA at 1.382 kb on - strand, within parA at 1.435 kb on + strand, within parA at 1.473 kb on + strand, within parA at 1.514 kb on + strand, within parA at 1.516 kb on - strand at 1.567 kb on + strand, within parA at 1.573 kb on - strand, within parA at 1.630 kb on + strand, within parA at 1.643 kb on - strand, within parA at 1.657 kb on - strand, within parA at 1.678 kb on - strand, within parA at 1.691 kb on - strand, within parA at 1.710 kb on - strand, within parA at 1.710 kb on - strand at 1.756 kb on + strand, within parA at 1.756 kb on + strand, within parA at 1.761 kb on + strand, within parA at 1.769 kb on - strand, within parA at 1.770 kb on + strand, within parA at 1.771 kb on - strand, within parA at 1.820 kb on + strand, within parA at 1.852 kb on + strand, within parA at 1.852 kb on + strand, within parA at 1.878 kb on + strand at 1.878 kb on + strand at 1.888 kb on + strand at 1.913 kb on + strand at 1.921 kb on - strand at 1.923 kb on + strand at 1.923 kb on + strand at 1.923 kb on + strand at 1.924 kb on + strand at 1.931 kb on - strand at 1.931 kb on - strand at 1.935 kb on + strand at 1.935 kb on + strand at 1.935 kb on + strand at 1.935 kb on + strand at 1.936 kb on + strand at 1.936 kb on + strand at 1.936 kb on + strand at 1.936 kb on + strand at 1.936 kb on + strand at 1.936 kb on + strand at 1.936 kb on + strand at 1.943 kb on - strand at 1.943 kb on - strand at 1.944 kb on - strand at 1.944 kb on - strand at 1.944 kb on - strand at 1.944 kb on - strand at 1.944 kb on - strand at 1.960 kb on + strand at 1.968 kb on - strand at 1.972 kb on + strand at 1.973 kb on + strand at 1.979 kb on + strand at 1.979 kb on + strand at 1.979 kb on + strand at 1.980 kb on - strand at 1.981 kb on - strand at 1.987 kb on - strand at 1.999 kb on + strand at 1.999 kb on + strand at 1.999 kb on + strand at 1.999 kb on + strand at 1.999 kb on + strand at 1.999 kb on + strand at 2.005 kb on - strand at 2.005 kb on - strand at 2.007 kb on - strand at 2.007 kb on - strand at 2.007 kb on - strand at 2.007 kb on - strand at 2.007 kb on - strand at 2.007 kb on - strand at 2.007 kb on - strand at 2.024 kb on + strand at 2.024 kb on + strand at 2.024 kb on + strand at 2.037 kb on + strand at 2.058 kb on - strand at 2.311 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Darkness for 120h then Flask growth in 60 uE constant light remove 18 + +0.1 42 + +0.1 51 - +0.8 52 + -0.3 53 + +0.2 60 - +0.6 60 - -0.7 70 - +0.2 70 - +0.1 71 - -0.5 72 + -0.3 77 + -0.0 85 - +0.0 88 + +0.0 96 - +0.0 133 + Synpcc7942_B2615 0.21 -0.2 133 + Synpcc7942_B2615 0.21 +0.5 133 + Synpcc7942_B2615 0.21 +0.2 133 + Synpcc7942_B2615 0.21 -0.1 141 - Synpcc7942_B2615 0.23 +0.3 141 - Synpcc7942_B2615 0.23 -0.2 143 + Synpcc7942_B2615 0.24 +0.2 143 + Synpcc7942_B2615 0.24 -0.2 143 + Synpcc7942_B2615 0.24 +0.3 151 - Synpcc7942_B2615 0.26 +0.3 151 - Synpcc7942_B2615 0.26 +0.7 151 - Synpcc7942_B2615 0.26 -0.0 151 - Synpcc7942_B2615 0.26 -0.0 151 - Synpcc7942_B2615 0.26 +0.1 158 + Synpcc7942_B2615 0.28 -0.8 158 + Synpcc7942_B2615 0.28 +0.6 166 - Synpcc7942_B2615 0.31 -1.6 207 - Synpcc7942_B2615 0.43 +0.0 207 - Synpcc7942_B2615 0.43 +0.1 214 + Synpcc7942_B2615 0.45 +0.0 221 + Synpcc7942_B2615 0.47 +0.0 221 + Synpcc7942_B2615 0.47 -1.3 221 + Synpcc7942_B2615 0.47 -0.5 229 - Synpcc7942_B2615 0.50 +1.1 229 - +1.0 229 - Synpcc7942_B2615 0.50 +0.6 229 - Synpcc7942_B2615 0.50 +0.4 229 - Synpcc7942_B2615 0.50 +0.3 229 - Synpcc7942_B2615 0.50 +0.2 240 + Synpcc7942_B2615 0.53 -0.4 247 + Synpcc7942_B2615 0.55 +0.1 247 + Synpcc7942_B2615 0.55 -0.3 248 - Synpcc7942_B2615 0.55 -0.2 255 - Synpcc7942_B2615 0.58 -1.2 255 - Synpcc7942_B2615 0.58 +0.1 255 - Synpcc7942_B2615 0.58 -0.4 255 - Synpcc7942_B2615 0.58 +0.2 255 - Synpcc7942_B2615 0.58 -2.6 257 + Synpcc7942_B2615 0.58 -0.2 257 + Synpcc7942_B2615 0.58 +0.2 257 + Synpcc7942_B2615 0.58 -0.3 304 - Synpcc7942_B2615 0.72 +0.0 322 + Synpcc7942_B2615 0.78 -0.0 360 + Synpcc7942_B2615 0.89 +0.3 360 + Synpcc7942_B2615 0.89 -0.1 360 + Synpcc7942_B2615 0.89 +0.2 373 + -0.5 373 + -0.8 373 + -0.4 373 + +0.1 373 + +0.3 373 + +0.5 373 + -1.2 373 + -0.1 373 + -0.5 373 + -0.4 381 - -0.6 381 - -1.1 381 - -0.6 381 - +0.1 381 - +0.4 381 - +0.7 396 + -0.2 401 + -0.3 401 + +0.1 401 + -0.2 401 + +0.1 401 + +1.1 404 - -0.0 404 - +0.2 407 - +0.5 409 - +0.4 409 - -0.8 409 - -0.7 414 - +1.0 414 - -0.0 414 - +0.0 431 + +0.8 495 + +0.1 495 + -0.7 495 + +0.2 495 + -0.0 510 + +0.4 512 + -0.4 514 + +0.6 565 - -1.1 601 - parB Synpcc7942_B2626 0.13 -1.3 651 + parB Synpcc7942_B2626 0.19 -0.8 680 + parB Synpcc7942_B2626 0.22 +0.1 748 + parB Synpcc7942_B2626 0.30 -1.8 756 - parB Synpcc7942_B2626 0.31 -1.3 855 + parB Synpcc7942_B2626 0.43 -0.9 877 - parB Synpcc7942_B2626 0.46 -3.9 1,007 + parB Synpcc7942_B2626 0.62 +0.5 1,192 - parB Synpcc7942_B2626 0.84 -1.4 1,218 + parB Synpcc7942_B2626 0.87 -0.1 1,293 - -0.5 1,293 - +0.1 1,295 + -0.2 1,303 - +0.0 1,340 - +1.3 1,362 - -0.6 1,380 + parA Synpcc7942_B2637 0.10 -1.3 1,380 + parA Synpcc7942_B2637 0.10 -1.2 1,382 - parA Synpcc7942_B2637 0.10 -1.6 1,435 + parA Synpcc7942_B2637 0.19 -0.2 1,473 + parA Synpcc7942_B2637 0.25 -1.0 1,514 + parA Synpcc7942_B2637 0.32 -1.0 1,516 - -0.9 1,567 + parA Synpcc7942_B2637 0.41 -0.3 1,573 - parA Synpcc7942_B2637 0.42 -0.5 1,630 + parA Synpcc7942_B2637 0.51 -1.1 1,643 - parA Synpcc7942_B2637 0.53 -0.8 1,657 - parA Synpcc7942_B2637 0.55 -0.1 1,678 - parA Synpcc7942_B2637 0.59 -1.9 1,691 - parA Synpcc7942_B2637 0.61 -1.1 1,710 - parA Synpcc7942_B2637 0.64 -2.6 1,710 - +0.7 1,756 + parA Synpcc7942_B2637 0.72 +0.1 1,756 + parA Synpcc7942_B2637 0.72 -1.1 1,761 + parA Synpcc7942_B2637 0.72 -1.3 1,769 - parA Synpcc7942_B2637 0.74 +0.7 1,770 + parA Synpcc7942_B2637 0.74 -1.6 1,771 - parA Synpcc7942_B2637 0.74 -0.5 1,820 + parA Synpcc7942_B2637 0.82 -0.4 1,852 + parA Synpcc7942_B2637 0.87 -0.8 1,852 + parA Synpcc7942_B2637 0.87 -0.9 1,878 + -1.5 1,878 + -0.9 1,888 + -0.1 1,913 + +0.2 1,921 - +0.8 1,923 + +0.3 1,923 + -0.1 1,923 + +0.0 1,924 + +0.1 1,931 - -0.1 1,931 - +0.1 1,935 + -1.0 1,935 + +0.2 1,935 + -0.1 1,935 + -1.2 1,936 + +0.0 1,936 + -0.1 1,936 + -0.1 1,936 + +1.3 1,936 + -0.3 1,936 + -0.3 1,936 + -0.5 1,943 - -0.2 1,943 - -0.2 1,944 - +0.4 1,944 - +0.5 1,944 - -0.6 1,944 - -0.3 1,944 - -1.3 1,960 + -0.5 1,968 - +0.7 1,972 + -0.0 1,973 + +0.7 1,979 + -1.5 1,979 + +0.1 1,979 + +0.7 1,980 - +0.3 1,981 - -0.2 1,987 - -1.1 1,999 + -0.7 1,999 + -0.5 1,999 + -0.1 1,999 + -0.5 1,999 + -1.1 1,999 + -0.2 2,005 - -0.9 2,005 - -0.2 2,007 - +0.2 2,007 - -1.5 2,007 - -0.4 2,007 - +0.4 2,007 - -0.7 2,007 - +0.0 2,007 - +0.4 2,024 + -1.8 2,024 + -0.8 2,024 + -1.2 2,037 + -2.5 2,058 - -0.4 2,311 + -1.9
Or see this region's nucleotide sequence