Experiment: Darkness for 120h then Flask growth in 60 uE constant light
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt psaA and Synpcc7942_2050 are separated by 364 nucleotides Synpcc7942_2050 and Synpcc7942_2051 overlap by 4 nucleotides
Synpcc7942_2049: psaA - photosystem I P700 chlorophyll a apoprotein A1, at 2,123,745 to 2,126,036
psaA
Synpcc7942_2050: Synpcc7942_2050 - hypothetical protein, at 2,126,401 to 2,127,864
_2050
Synpcc7942_2051: Synpcc7942_2051 - hypothetical protein, at 2,127,861 to 2,129,303
_2051
Position (kb)
2126
2127
2128 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2126.286 kb on - strand at 2126.389 kb on + strand at 2126.397 kb on - strand at 2126.397 kb on - strand at 2126.397 kb on - strand at 2126.397 kb on - strand at 2126.457 kb on + strand at 2126.503 kb on + strand at 2126.508 kb on + strand at 2126.527 kb on + strand at 2126.527 kb on + strand at 2126.535 kb on - strand at 2126.548 kb on - strand, within Synpcc7942_2050 at 2126.552 kb on + strand, within Synpcc7942_2050 at 2126.560 kb on - strand, within Synpcc7942_2050 at 2126.562 kb on + strand, within Synpcc7942_2050 at 2126.635 kb on + strand, within Synpcc7942_2050 at 2126.682 kb on + strand, within Synpcc7942_2050 at 2126.751 kb on + strand, within Synpcc7942_2050 at 2126.766 kb on + strand, within Synpcc7942_2050 at 2126.845 kb on - strand, within Synpcc7942_2050 at 2126.948 kb on - strand, within Synpcc7942_2050 at 2126.951 kb on + strand, within Synpcc7942_2050 at 2126.954 kb on + strand, within Synpcc7942_2050 at 2126.962 kb on - strand, within Synpcc7942_2050 at 2126.962 kb on - strand, within Synpcc7942_2050 at 2126.982 kb on + strand, within Synpcc7942_2050 at 2126.990 kb on - strand, within Synpcc7942_2050 at 2127.048 kb on + strand, within Synpcc7942_2050 at 2127.071 kb on - strand, within Synpcc7942_2050 at 2127.088 kb on + strand, within Synpcc7942_2050 at 2127.100 kb on + strand, within Synpcc7942_2050 at 2127.167 kb on - strand, within Synpcc7942_2050 at 2127.222 kb on - strand at 2127.223 kb on + strand, within Synpcc7942_2050 at 2127.268 kb on - strand, within Synpcc7942_2050 at 2127.276 kb on - strand at 2127.278 kb on - strand, within Synpcc7942_2050 at 2127.339 kb on + strand, within Synpcc7942_2050 at 2127.347 kb on - strand, within Synpcc7942_2050 at 2127.385 kb on + strand, within Synpcc7942_2050 at 2127.458 kb on - strand, within Synpcc7942_2050 at 2127.514 kb on - strand, within Synpcc7942_2050 at 2127.566 kb on + strand, within Synpcc7942_2050 at 2127.566 kb on + strand at 2127.574 kb on - strand, within Synpcc7942_2050 at 2127.576 kb on + strand, within Synpcc7942_2050 at 2127.597 kb on + strand, within Synpcc7942_2050 at 2127.597 kb on + strand, within Synpcc7942_2050 at 2127.607 kb on - strand, within Synpcc7942_2050 at 2127.607 kb on - strand, within Synpcc7942_2050 at 2127.609 kb on + strand, within Synpcc7942_2050 at 2127.619 kb on + strand, within Synpcc7942_2050 at 2127.688 kb on + strand, within Synpcc7942_2050 at 2127.738 kb on + strand at 2127.738 kb on + strand at 2127.746 kb on - strand at 2127.746 kb on - strand at 2127.748 kb on - strand at 2127.758 kb on - strand at 2127.779 kb on - strand at 2127.793 kb on + strand at 2127.794 kb on - strand at 2127.803 kb on + strand at 2127.825 kb on - strand at 2127.844 kb on + strand at 2127.852 kb on - strand at 2127.862 kb on - strand at 2127.908 kb on + strand at 2127.974 kb on - strand at 2127.979 kb on - strand at 2127.994 kb on - strand at 2127.997 kb on + strand at 2128.005 kb on - strand at 2128.010 kb on + strand, within Synpcc7942_2051 at 2128.016 kb on + strand, within Synpcc7942_2051 at 2128.041 kb on - strand, within Synpcc7942_2051 at 2128.102 kb on - strand, within Synpcc7942_2051 at 2128.107 kb on - strand, within Synpcc7942_2051 at 2128.109 kb on + strand, within Synpcc7942_2051 at 2128.123 kb on - strand, within Synpcc7942_2051 at 2128.136 kb on + strand, within Synpcc7942_2051 at 2128.173 kb on + strand, within Synpcc7942_2051 at 2128.183 kb on + strand, within Synpcc7942_2051 at 2128.192 kb on - strand, within Synpcc7942_2051 at 2128.207 kb on + strand, within Synpcc7942_2051 at 2128.207 kb on + strand at 2128.207 kb on + strand, within Synpcc7942_2051 at 2128.207 kb on + strand, within Synpcc7942_2051 at 2128.207 kb on + strand, within Synpcc7942_2051 at 2128.207 kb on + strand, within Synpcc7942_2051 at 2128.210 kb on - strand, within Synpcc7942_2051 at 2128.215 kb on - strand, within Synpcc7942_2051 at 2128.215 kb on - strand, within Synpcc7942_2051 at 2128.215 kb on - strand, within Synpcc7942_2051 at 2128.215 kb on - strand, within Synpcc7942_2051 at 2128.215 kb on - strand, within Synpcc7942_2051 at 2128.215 kb on - strand, within Synpcc7942_2051 at 2128.221 kb on - strand, within Synpcc7942_2051 at 2128.227 kb on - strand, within Synpcc7942_2051 at 2128.249 kb on - strand, within Synpcc7942_2051 at 2128.257 kb on + strand, within Synpcc7942_2051 at 2128.257 kb on + strand, within Synpcc7942_2051 at 2128.312 kb on + strand, within Synpcc7942_2051 at 2128.313 kb on + strand, within Synpcc7942_2051 at 2128.325 kb on + strand, within Synpcc7942_2051 at 2128.325 kb on + strand, within Synpcc7942_2051 at 2128.337 kb on - strand, within Synpcc7942_2051 at 2128.345 kb on - strand, within Synpcc7942_2051 at 2128.391 kb on + strand, within Synpcc7942_2051 at 2128.399 kb on + strand, within Synpcc7942_2051 at 2128.405 kb on - strand, within Synpcc7942_2051 at 2128.481 kb on - strand, within Synpcc7942_2051 at 2128.481 kb on - strand, within Synpcc7942_2051 at 2128.491 kb on - strand, within Synpcc7942_2051 at 2128.491 kb on - strand, within Synpcc7942_2051 at 2128.519 kb on - strand, within Synpcc7942_2051 at 2128.532 kb on + strand, within Synpcc7942_2051 at 2128.540 kb on - strand, within Synpcc7942_2051 at 2128.551 kb on - strand, within Synpcc7942_2051 at 2128.587 kb on + strand, within Synpcc7942_2051 at 2128.595 kb on - strand, within Synpcc7942_2051 at 2128.610 kb on + strand, within Synpcc7942_2051 at 2128.618 kb on - strand, within Synpcc7942_2051 at 2128.618 kb on - strand, within Synpcc7942_2051 at 2128.643 kb on + strand, within Synpcc7942_2051 at 2128.651 kb on - strand, within Synpcc7942_2051 at 2128.651 kb on - strand, within Synpcc7942_2051 at 2128.651 kb on - strand, within Synpcc7942_2051 at 2128.651 kb on - strand, within Synpcc7942_2051 at 2128.651 kb on - strand, within Synpcc7942_2051 at 2128.662 kb on + strand, within Synpcc7942_2051 at 2128.670 kb on + strand, within Synpcc7942_2051 at 2128.753 kb on - strand, within Synpcc7942_2051 at 2128.793 kb on - strand, within Synpcc7942_2051 at 2128.817 kb on + strand at 2128.824 kb on + strand, within Synpcc7942_2051 at 2128.830 kb on + strand, within Synpcc7942_2051 at 2128.838 kb on - strand, within Synpcc7942_2051 at 2128.846 kb on + strand, within Synpcc7942_2051 at 2128.858 kb on + strand, within Synpcc7942_2051
Per-strain Table
Position Strand Gene LocusTag Fraction Darkness for 120h then Flask growth in 60 uE constant light remove 2,126,286 - +0.3 2,126,389 + -0.9 2,126,397 - -0.0 2,126,397 - +0.5 2,126,397 - -0.7 2,126,397 - -0.7 2,126,457 + -0.2 2,126,503 + -0.4 2,126,508 + -1.3 2,126,527 + +0.1 2,126,527 + -0.0 2,126,535 - -0.3 2,126,548 - Synpcc7942_2050 0.10 +0.1 2,126,552 + Synpcc7942_2050 0.10 -0.6 2,126,560 - Synpcc7942_2050 0.11 +0.1 2,126,562 + Synpcc7942_2050 0.11 -1.1 2,126,635 + Synpcc7942_2050 0.16 -0.8 2,126,682 + Synpcc7942_2050 0.19 -0.9 2,126,751 + Synpcc7942_2050 0.24 -1.8 2,126,766 + Synpcc7942_2050 0.25 -1.8 2,126,845 - Synpcc7942_2050 0.30 -1.6 2,126,948 - Synpcc7942_2050 0.37 -1.8 2,126,951 + Synpcc7942_2050 0.38 -1.6 2,126,954 + Synpcc7942_2050 0.38 -1.3 2,126,962 - Synpcc7942_2050 0.38 -2.1 2,126,962 - Synpcc7942_2050 0.38 -0.5 2,126,982 + Synpcc7942_2050 0.40 -1.5 2,126,990 - Synpcc7942_2050 0.40 -0.2 2,127,048 + Synpcc7942_2050 0.44 -1.1 2,127,071 - Synpcc7942_2050 0.46 -2.3 2,127,088 + Synpcc7942_2050 0.47 -3.0 2,127,100 + Synpcc7942_2050 0.48 -0.3 2,127,167 - Synpcc7942_2050 0.52 -1.8 2,127,222 - -0.0 2,127,223 + Synpcc7942_2050 0.56 -0.9 2,127,268 - Synpcc7942_2050 0.59 -1.3 2,127,276 - -1.0 2,127,278 - Synpcc7942_2050 0.60 -2.4 2,127,339 + Synpcc7942_2050 0.64 -2.0 2,127,347 - Synpcc7942_2050 0.65 -1.9 2,127,385 + Synpcc7942_2050 0.67 -2.3 2,127,458 - Synpcc7942_2050 0.72 -1.2 2,127,514 - Synpcc7942_2050 0.76 -0.7 2,127,566 + Synpcc7942_2050 0.80 +0.2 2,127,566 + +0.3 2,127,574 - Synpcc7942_2050 0.80 -0.2 2,127,576 + Synpcc7942_2050 0.80 -0.4 2,127,597 + Synpcc7942_2050 0.82 -0.3 2,127,597 + Synpcc7942_2050 0.82 -0.1 2,127,607 - Synpcc7942_2050 0.82 -0.4 2,127,607 - Synpcc7942_2050 0.82 -0.2 2,127,609 + Synpcc7942_2050 0.83 +0.3 2,127,619 + Synpcc7942_2050 0.83 -0.2 2,127,688 + Synpcc7942_2050 0.88 +1.1 2,127,738 + -0.4 2,127,738 + -0.3 2,127,746 - -0.3 2,127,746 - -1.1 2,127,748 - -0.6 2,127,758 - +0.1 2,127,779 - +0.6 2,127,793 + -0.4 2,127,794 - -0.6 2,127,803 + +0.6 2,127,825 - +0.1 2,127,844 + -0.6 2,127,852 - -0.3 2,127,862 - -0.8 2,127,908 + +0.0 2,127,974 - -0.5 2,127,979 - -0.2 2,127,994 - -0.4 2,127,997 + -0.3 2,128,005 - -0.1 2,128,010 + Synpcc7942_2051 0.10 -0.4 2,128,016 + Synpcc7942_2051 0.11 +0.6 2,128,041 - Synpcc7942_2051 0.12 -0.4 2,128,102 - Synpcc7942_2051 0.17 -0.6 2,128,107 - Synpcc7942_2051 0.17 +0.4 2,128,109 + Synpcc7942_2051 0.17 +0.0 2,128,123 - Synpcc7942_2051 0.18 +0.3 2,128,136 + Synpcc7942_2051 0.19 -0.3 2,128,173 + Synpcc7942_2051 0.22 -0.5 2,128,183 + Synpcc7942_2051 0.22 -0.2 2,128,192 - Synpcc7942_2051 0.23 +0.4 2,128,207 + Synpcc7942_2051 0.24 -1.0 2,128,207 + +0.4 2,128,207 + Synpcc7942_2051 0.24 -1.3 2,128,207 + Synpcc7942_2051 0.24 +0.4 2,128,207 + Synpcc7942_2051 0.24 -1.0 2,128,207 + Synpcc7942_2051 0.24 -0.2 2,128,210 - Synpcc7942_2051 0.24 -0.0 2,128,215 - Synpcc7942_2051 0.25 +2.0 2,128,215 - Synpcc7942_2051 0.25 +0.7 2,128,215 - Synpcc7942_2051 0.25 +0.7 2,128,215 - Synpcc7942_2051 0.25 +0.1 2,128,215 - Synpcc7942_2051 0.25 -0.6 2,128,215 - Synpcc7942_2051 0.25 +0.2 2,128,221 - Synpcc7942_2051 0.25 -0.0 2,128,227 - Synpcc7942_2051 0.25 -0.8 2,128,249 - Synpcc7942_2051 0.27 -0.0 2,128,257 + Synpcc7942_2051 0.27 -0.5 2,128,257 + Synpcc7942_2051 0.27 -0.1 2,128,312 + Synpcc7942_2051 0.31 +0.6 2,128,313 + Synpcc7942_2051 0.31 -0.3 2,128,325 + Synpcc7942_2051 0.32 +0.0 2,128,325 + Synpcc7942_2051 0.32 -0.1 2,128,337 - Synpcc7942_2051 0.33 +0.1 2,128,345 - Synpcc7942_2051 0.34 +0.2 2,128,391 + Synpcc7942_2051 0.37 -1.9 2,128,399 + Synpcc7942_2051 0.37 -0.4 2,128,405 - Synpcc7942_2051 0.38 +0.3 2,128,481 - Synpcc7942_2051 0.43 +0.1 2,128,481 - Synpcc7942_2051 0.43 -0.2 2,128,491 - Synpcc7942_2051 0.44 +0.2 2,128,491 - Synpcc7942_2051 0.44 -0.1 2,128,519 - Synpcc7942_2051 0.46 +0.3 2,128,532 + Synpcc7942_2051 0.47 -0.2 2,128,540 - Synpcc7942_2051 0.47 -0.4 2,128,551 - Synpcc7942_2051 0.48 +0.6 2,128,587 + Synpcc7942_2051 0.50 -0.7 2,128,595 - Synpcc7942_2051 0.51 +0.3 2,128,610 + Synpcc7942_2051 0.52 +1.4 2,128,618 - Synpcc7942_2051 0.52 +0.4 2,128,618 - Synpcc7942_2051 0.52 +0.4 2,128,643 + Synpcc7942_2051 0.54 +0.2 2,128,651 - Synpcc7942_2051 0.55 -0.2 2,128,651 - Synpcc7942_2051 0.55 +0.2 2,128,651 - Synpcc7942_2051 0.55 +0.5 2,128,651 - Synpcc7942_2051 0.55 +0.8 2,128,651 - Synpcc7942_2051 0.55 +0.5 2,128,662 + Synpcc7942_2051 0.56 -0.5 2,128,670 + Synpcc7942_2051 0.56 -0.1 2,128,753 - Synpcc7942_2051 0.62 -0.2 2,128,793 - Synpcc7942_2051 0.65 +0.9 2,128,817 + +0.1 2,128,824 + Synpcc7942_2051 0.67 -0.2 2,128,830 + Synpcc7942_2051 0.67 +0.0 2,128,838 - Synpcc7942_2051 0.68 +0.6 2,128,846 + Synpcc7942_2051 0.68 -0.7 2,128,858 + Synpcc7942_2051 0.69 -0.8
Or see this region's nucleotide sequence