Experiment: Darkness for 120h then Flask growth in 60 uE constant light
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt natB and Synpcc7942_1862 are separated by 159 nucleotides Synpcc7942_1862 and hemG are separated by 12 nucleotides hemG and Synpcc7942_1864 are separated by 5 nucleotides
Synpcc7942_1861: natB - periplasmic binding protein of ABC transporter for natural amino acids, at 1,931,340 to 1,932,605
natB
Synpcc7942_1862: Synpcc7942_1862 - hypothetical protein, at 1,932,765 to 1,933,688
_1862
Synpcc7942_1863: hemG - modification methylase, HemK family, at 1,933,701 to 1,934,582
hemG
Synpcc7942_1864: Synpcc7942_1864 - hypothetical protein, at 1,934,588 to 1,935,175
_1864
Position (kb) 1932
1933
1934 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 1931.778 kb on - strand, within natB at 1931.788 kb on + strand, within natB at 1931.790 kb on + strand, within natB at 1931.790 kb on + strand, within natB at 1931.796 kb on - strand, within natB at 1931.798 kb on - strand, within natB at 1931.811 kb on + strand, within natB at 1931.825 kb on + strand, within natB at 1931.834 kb on - strand, within natB at 1931.840 kb on - strand, within natB at 1931.887 kb on + strand, within natB at 1931.899 kb on + strand, within natB at 1931.899 kb on + strand, within natB at 1931.932 kb on - strand, within natB at 1931.937 kb on + strand, within natB at 1931.945 kb on - strand, within natB at 1931.949 kb on + strand, within natB at 1931.951 kb on + strand, within natB at 1931.956 kb on + strand, within natB at 1931.959 kb on + strand, within natB at 1931.959 kb on - strand, within natB at 1931.961 kb on + strand, within natB at 1931.964 kb on - strand, within natB at 1931.967 kb on - strand, within natB at 1931.982 kb on + strand, within natB at 1931.990 kb on - strand, within natB at 1932.003 kb on - strand at 1932.005 kb on + strand at 1932.013 kb on - strand, within natB at 1932.035 kb on + strand, within natB at 1932.038 kb on - strand, within natB at 1932.066 kb on + strand, within natB at 1932.074 kb on - strand, within natB at 1932.085 kb on + strand, within natB at 1932.118 kb on - strand, within natB at 1932.118 kb on - strand, within natB at 1932.118 kb on - strand, within natB at 1932.123 kb on - strand, within natB at 1932.128 kb on + strand, within natB at 1932.132 kb on + strand, within natB at 1932.132 kb on + strand, within natB at 1932.191 kb on + strand, within natB at 1932.224 kb on + strand, within natB at 1932.231 kb on + strand, within natB at 1932.231 kb on + strand, within natB at 1932.231 kb on + strand, within natB at 1932.231 kb on + strand, within natB at 1932.246 kb on + strand, within natB at 1932.246 kb on + strand, within natB at 1932.246 kb on + strand, within natB at 1932.246 kb on + strand, within natB at 1932.246 kb on + strand, within natB at 1932.246 kb on + strand, within natB at 1932.246 kb on + strand, within natB at 1932.246 kb on + strand, within natB at 1932.247 kb on - strand, within natB at 1932.254 kb on - strand, within natB at 1932.254 kb on - strand, within natB at 1932.255 kb on - strand, within natB at 1932.260 kb on - strand, within natB at 1932.271 kb on - strand, within natB at 1932.298 kb on - strand, within natB at 1932.307 kb on + strand, within natB at 1932.316 kb on + strand, within natB at 1932.329 kb on - strand, within natB at 1932.352 kb on + strand, within natB at 1932.382 kb on + strand, within natB at 1932.387 kb on + strand, within natB at 1932.387 kb on + strand, within natB at 1932.395 kb on - strand, within natB at 1932.395 kb on - strand, within natB at 1932.405 kb on + strand, within natB at 1932.405 kb on + strand, within natB at 1932.405 kb on + strand, within natB at 1932.423 kb on + strand, within natB at 1932.428 kb on + strand, within natB at 1932.428 kb on + strand, within natB at 1932.438 kb on + strand, within natB at 1932.446 kb on - strand, within natB at 1932.446 kb on - strand, within natB at 1932.446 kb on - strand, within natB at 1932.461 kb on - strand, within natB at 1932.496 kb on + strand at 1932.505 kb on - strand at 1932.514 kb on - strand at 1932.549 kb on + strand at 1932.557 kb on + strand at 1932.560 kb on + strand at 1932.560 kb on - strand at 1932.562 kb on + strand at 1932.570 kb on - strand at 1932.580 kb on - strand at 1932.580 kb on - strand at 1932.610 kb on - strand at 1932.611 kb on + strand at 1932.611 kb on + strand at 1932.619 kb on - strand at 1932.628 kb on + strand at 1932.644 kb on + strand at 1932.696 kb on + strand at 1932.696 kb on + strand at 1932.707 kb on + strand at 1932.707 kb on + strand at 1932.715 kb on - strand at 1932.719 kb on + strand at 1932.726 kb on + strand at 1932.727 kb on - strand at 1932.817 kb on - strand at 1932.835 kb on - strand at 1932.892 kb on - strand, within Synpcc7942_1862 at 1932.902 kb on - strand, within Synpcc7942_1862 at 1932.927 kb on - strand, within Synpcc7942_1862 at 1933.126 kb on + strand, within Synpcc7942_1862 at 1933.141 kb on - strand, within Synpcc7942_1862 at 1933.402 kb on - strand, within Synpcc7942_1862 at 1933.449 kb on + strand, within Synpcc7942_1862 at 1933.487 kb on + strand, within Synpcc7942_1862 at 1933.565 kb on + strand, within Synpcc7942_1862 at 1933.657 kb on + strand at 1933.660 kb on - strand at 1933.663 kb on + strand at 1933.663 kb on + strand at 1933.663 kb on + strand at 1933.665 kb on - strand at 1933.671 kb on - strand at 1933.738 kb on - strand at 1933.779 kb on + strand at 1933.801 kb on - strand, within hemG at 1933.824 kb on + strand, within hemG at 1933.837 kb on - strand, within hemG at 1933.842 kb on - strand, within hemG at 1933.852 kb on + strand, within hemG at 1933.857 kb on + strand, within hemG at 1933.857 kb on + strand, within hemG at 1933.862 kb on + strand, within hemG at 1933.870 kb on - strand, within hemG at 1933.928 kb on + strand, within hemG at 1933.928 kb on + strand, within hemG at 1933.928 kb on + strand, within hemG at 1933.936 kb on - strand, within hemG at 1933.936 kb on - strand, within hemG at 1933.936 kb on - strand, within hemG at 1933.936 kb on - strand, within hemG at 1933.947 kb on - strand, within hemG at 1933.991 kb on - strand, within hemG at 1934.039 kb on - strand, within hemG at 1934.044 kb on - strand, within hemG at 1934.076 kb on + strand, within hemG at 1934.076 kb on + strand, within hemG at 1934.076 kb on + strand, within hemG at 1934.084 kb on - strand, within hemG at 1934.084 kb on - strand, within hemG at 1934.146 kb on + strand, within hemG at 1934.178 kb on + strand, within hemG at 1934.183 kb on + strand, within hemG at 1934.191 kb on - strand, within hemG at 1934.225 kb on + strand, within hemG at 1934.226 kb on + strand, within hemG at 1934.229 kb on - strand, within hemG at 1934.234 kb on + strand, within hemG at 1934.240 kb on + strand, within hemG at 1934.260 kb on + strand, within hemG at 1934.330 kb on - strand, within hemG at 1934.344 kb on + strand, within hemG at 1934.344 kb on + strand, within hemG at 1934.344 kb on + strand, within hemG at 1934.352 kb on - strand, within hemG at 1934.352 kb on - strand, within hemG at 1934.362 kb on - strand, within hemG at 1934.374 kb on + strand, within hemG at 1934.391 kb on + strand, within hemG at 1934.440 kb on + strand, within hemG at 1934.440 kb on + strand, within hemG at 1934.440 kb on + strand, within hemG at 1934.465 kb on - strand, within hemG at 1934.520 kb on - strand at 1934.529 kb on - strand at 1934.567 kb on + strand at 1934.577 kb on + strand at 1934.577 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Darkness for 120h then Flask growth in 60 uE constant light remove 1,931,778 - natB Synpcc7942_1861 0.35 +1.1 1,931,788 + natB Synpcc7942_1861 0.35 -0.2 1,931,790 + natB Synpcc7942_1861 0.36 -0.0 1,931,790 + natB Synpcc7942_1861 0.36 +0.7 1,931,796 - natB Synpcc7942_1861 0.36 +0.3 1,931,798 - natB Synpcc7942_1861 0.36 -0.7 1,931,811 + natB Synpcc7942_1861 0.37 +0.2 1,931,825 + natB Synpcc7942_1861 0.38 +0.4 1,931,834 - natB Synpcc7942_1861 0.39 +1.0 1,931,840 - natB Synpcc7942_1861 0.39 +0.6 1,931,887 + natB Synpcc7942_1861 0.43 -0.5 1,931,899 + natB Synpcc7942_1861 0.44 +0.2 1,931,899 + natB Synpcc7942_1861 0.44 -0.1 1,931,932 - natB Synpcc7942_1861 0.47 -1.0 1,931,937 + natB Synpcc7942_1861 0.47 +0.4 1,931,945 - natB Synpcc7942_1861 0.48 +0.7 1,931,949 + natB Synpcc7942_1861 0.48 -0.3 1,931,951 + natB Synpcc7942_1861 0.48 +0.2 1,931,956 + natB Synpcc7942_1861 0.49 +0.4 1,931,959 + natB Synpcc7942_1861 0.49 -0.4 1,931,959 - natB Synpcc7942_1861 0.49 +0.1 1,931,961 + natB Synpcc7942_1861 0.49 +0.3 1,931,964 - natB Synpcc7942_1861 0.49 +0.8 1,931,967 - natB Synpcc7942_1861 0.50 +0.5 1,931,982 + natB Synpcc7942_1861 0.51 +0.1 1,931,990 - natB Synpcc7942_1861 0.51 +0.1 1,932,003 - -0.3 1,932,005 + +1.0 1,932,013 - natB Synpcc7942_1861 0.53 +0.6 1,932,035 + natB Synpcc7942_1861 0.55 +0.1 1,932,038 - natB Synpcc7942_1861 0.55 -0.7 1,932,066 + natB Synpcc7942_1861 0.57 +0.7 1,932,074 - natB Synpcc7942_1861 0.58 -0.0 1,932,085 + natB Synpcc7942_1861 0.59 -0.3 1,932,118 - natB Synpcc7942_1861 0.61 -0.1 1,932,118 - natB Synpcc7942_1861 0.61 -0.3 1,932,118 - natB Synpcc7942_1861 0.61 +0.7 1,932,123 - natB Synpcc7942_1861 0.62 +0.1 1,932,128 + natB Synpcc7942_1861 0.62 +0.5 1,932,132 + natB Synpcc7942_1861 0.63 -0.9 1,932,132 + natB Synpcc7942_1861 0.63 +0.1 1,932,191 + natB Synpcc7942_1861 0.67 -0.0 1,932,224 + natB Synpcc7942_1861 0.70 -0.2 1,932,231 + natB Synpcc7942_1861 0.70 +0.9 1,932,231 + natB Synpcc7942_1861 0.70 +0.2 1,932,231 + natB Synpcc7942_1861 0.70 -0.1 1,932,231 + natB Synpcc7942_1861 0.70 -0.1 1,932,246 + natB Synpcc7942_1861 0.72 +0.5 1,932,246 + natB Synpcc7942_1861 0.72 +0.8 1,932,246 + natB Synpcc7942_1861 0.72 +0.7 1,932,246 + natB Synpcc7942_1861 0.72 +0.4 1,932,246 + natB Synpcc7942_1861 0.72 -0.8 1,932,246 + natB Synpcc7942_1861 0.72 +0.4 1,932,246 + natB Synpcc7942_1861 0.72 +0.4 1,932,246 + natB Synpcc7942_1861 0.72 +0.3 1,932,247 - natB Synpcc7942_1861 0.72 -0.1 1,932,254 - natB Synpcc7942_1861 0.72 +0.8 1,932,254 - natB Synpcc7942_1861 0.72 -0.5 1,932,255 - natB Synpcc7942_1861 0.72 -0.2 1,932,260 - natB Synpcc7942_1861 0.73 -0.8 1,932,271 - natB Synpcc7942_1861 0.74 -0.6 1,932,298 - natB Synpcc7942_1861 0.76 -0.0 1,932,307 + natB Synpcc7942_1861 0.76 -0.7 1,932,316 + natB Synpcc7942_1861 0.77 -0.8 1,932,329 - natB Synpcc7942_1861 0.78 +0.6 1,932,352 + natB Synpcc7942_1861 0.80 -0.4 1,932,382 + natB Synpcc7942_1861 0.82 +1.0 1,932,387 + natB Synpcc7942_1861 0.83 +0.1 1,932,387 + natB Synpcc7942_1861 0.83 -0.1 1,932,395 - natB Synpcc7942_1861 0.83 -0.4 1,932,395 - natB Synpcc7942_1861 0.83 -0.1 1,932,405 + natB Synpcc7942_1861 0.84 -0.4 1,932,405 + natB Synpcc7942_1861 0.84 -1.1 1,932,405 + natB Synpcc7942_1861 0.84 +0.5 1,932,423 + natB Synpcc7942_1861 0.86 -0.9 1,932,428 + natB Synpcc7942_1861 0.86 -0.4 1,932,428 + natB Synpcc7942_1861 0.86 +0.0 1,932,438 + natB Synpcc7942_1861 0.87 -1.1 1,932,446 - natB Synpcc7942_1861 0.87 -0.7 1,932,446 - natB Synpcc7942_1861 0.87 -0.4 1,932,446 - natB Synpcc7942_1861 0.87 +0.5 1,932,461 - natB Synpcc7942_1861 0.89 +0.6 1,932,496 + -0.1 1,932,505 - +0.1 1,932,514 - -0.0 1,932,549 + -0.3 1,932,557 + +0.1 1,932,560 + -0.0 1,932,560 - -1.0 1,932,562 + -0.1 1,932,570 - -0.6 1,932,580 - +0.9 1,932,580 - -1.4 1,932,610 - -0.1 1,932,611 + -0.1 1,932,611 + +0.3 1,932,619 - -0.5 1,932,628 + -0.2 1,932,644 + -0.6 1,932,696 + -0.4 1,932,696 + +0.4 1,932,707 + -1.7 1,932,707 + -1.3 1,932,715 - -1.6 1,932,719 + -0.7 1,932,726 + -1.5 1,932,727 - -1.8 1,932,817 - -3.4 1,932,835 - -1.0 1,932,892 - Synpcc7942_1862 0.14 -4.0 1,932,902 - Synpcc7942_1862 0.15 -2.9 1,932,927 - Synpcc7942_1862 0.18 -3.2 1,933,126 + Synpcc7942_1862 0.39 -4.0 1,933,141 - Synpcc7942_1862 0.41 -3.5 1,933,402 - Synpcc7942_1862 0.69 -3.3 1,933,449 + Synpcc7942_1862 0.74 -0.9 1,933,487 + Synpcc7942_1862 0.78 -1.6 1,933,565 + Synpcc7942_1862 0.87 -1.5 1,933,657 + -1.0 1,933,660 - -0.8 1,933,663 + -0.4 1,933,663 + -0.7 1,933,663 + -1.0 1,933,665 - -0.7 1,933,671 - -0.2 1,933,738 - -0.8 1,933,779 + +0.1 1,933,801 - hemG Synpcc7942_1863 0.11 +0.4 1,933,824 + hemG Synpcc7942_1863 0.14 -0.1 1,933,837 - hemG Synpcc7942_1863 0.15 +0.1 1,933,842 - hemG Synpcc7942_1863 0.16 -0.7 1,933,852 + hemG Synpcc7942_1863 0.17 +0.5 1,933,857 + hemG Synpcc7942_1863 0.18 +0.7 1,933,857 + hemG Synpcc7942_1863 0.18 +0.2 1,933,862 + hemG Synpcc7942_1863 0.18 -2.2 1,933,870 - hemG Synpcc7942_1863 0.19 +0.5 1,933,928 + hemG Synpcc7942_1863 0.26 +1.0 1,933,928 + hemG Synpcc7942_1863 0.26 +0.1 1,933,928 + hemG Synpcc7942_1863 0.26 -1.5 1,933,936 - hemG Synpcc7942_1863 0.27 +0.7 1,933,936 - hemG Synpcc7942_1863 0.27 -0.2 1,933,936 - hemG Synpcc7942_1863 0.27 +0.2 1,933,936 - hemG Synpcc7942_1863 0.27 -0.2 1,933,947 - hemG Synpcc7942_1863 0.28 -0.0 1,933,991 - hemG Synpcc7942_1863 0.33 -0.2 1,934,039 - hemG Synpcc7942_1863 0.38 -1.6 1,934,044 - hemG Synpcc7942_1863 0.39 -0.7 1,934,076 + hemG Synpcc7942_1863 0.43 -0.2 1,934,076 + hemG Synpcc7942_1863 0.43 -1.2 1,934,076 + hemG Synpcc7942_1863 0.43 +0.5 1,934,084 - hemG Synpcc7942_1863 0.43 -1.2 1,934,084 - hemG Synpcc7942_1863 0.43 +1.0 1,934,146 + hemG Synpcc7942_1863 0.50 +0.8 1,934,178 + hemG Synpcc7942_1863 0.54 -0.0 1,934,183 + hemG Synpcc7942_1863 0.55 -0.1 1,934,191 - hemG Synpcc7942_1863 0.56 -0.8 1,934,225 + hemG Synpcc7942_1863 0.59 +0.7 1,934,226 + hemG Synpcc7942_1863 0.60 -0.1 1,934,229 - hemG Synpcc7942_1863 0.60 -0.5 1,934,234 + hemG Synpcc7942_1863 0.60 +0.1 1,934,240 + hemG Synpcc7942_1863 0.61 -0.4 1,934,260 + hemG Synpcc7942_1863 0.63 +0.1 1,934,330 - hemG Synpcc7942_1863 0.71 +0.2 1,934,344 + hemG Synpcc7942_1863 0.73 -0.6 1,934,344 + hemG Synpcc7942_1863 0.73 -0.3 1,934,344 + hemG Synpcc7942_1863 0.73 +0.9 1,934,352 - hemG Synpcc7942_1863 0.74 -0.2 1,934,352 - hemG Synpcc7942_1863 0.74 +1.0 1,934,362 - hemG Synpcc7942_1863 0.75 +0.4 1,934,374 + hemG Synpcc7942_1863 0.76 -0.2 1,934,391 + hemG Synpcc7942_1863 0.78 +0.3 1,934,440 + hemG Synpcc7942_1863 0.84 -0.4 1,934,440 + hemG Synpcc7942_1863 0.84 +0.0 1,934,440 + hemG Synpcc7942_1863 0.84 -0.8 1,934,465 - hemG Synpcc7942_1863 0.87 -0.9 1,934,520 - +0.2 1,934,529 - -0.1 1,934,567 + +0.1 1,934,577 + +0.3 1,934,577 + -0.0
Or see this region's nucleotide sequence