Experiment: Darkness for 120h then Flask growth in 60 uE constant light
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_1125 and Synpcc7942_1126 are separated by 57 nucleotides Synpcc7942_1126 and tldD are separated by 149 nucleotides
Synpcc7942_1125: Synpcc7942_1125 - putative CheA signal transduction histidine kinase, no good domain identification, at 1,143,397 to 1,144,332
_1125
Synpcc7942_1126: Synpcc7942_1126 - ABC transporter permease protein, at 1,144,390 to 1,145,223
_1126
Synpcc7942_1127: tldD - microcin-processing peptidase 2. Unknown type peptidase. MEROPS family U62, at 1,145,373 to 1,146,842
tldD
Position (kb)
1144
1145
1146 Strain fitness (log2 ratio)
-2
-1
0
1 at 1143.463 kb on + strand at 1143.463 kb on + strand at 1143.471 kb on - strand at 1143.472 kb on + strand at 1143.480 kb on - strand at 1143.542 kb on + strand, within Synpcc7942_1125 at 1143.542 kb on + strand, within Synpcc7942_1125 at 1143.544 kb on + strand, within Synpcc7942_1125 at 1143.544 kb on + strand, within Synpcc7942_1125 at 1143.544 kb on + strand, within Synpcc7942_1125 at 1143.544 kb on + strand, within Synpcc7942_1125 at 1143.550 kb on - strand, within Synpcc7942_1125 at 1143.550 kb on - strand, within Synpcc7942_1125 at 1143.550 kb on - strand, within Synpcc7942_1125 at 1143.552 kb on - strand, within Synpcc7942_1125 at 1143.552 kb on - strand, within Synpcc7942_1125 at 1143.552 kb on - strand, within Synpcc7942_1125 at 1143.560 kb on - strand, within Synpcc7942_1125 at 1143.564 kb on - strand, within Synpcc7942_1125 at 1143.568 kb on + strand, within Synpcc7942_1125 at 1143.611 kb on + strand, within Synpcc7942_1125 at 1143.611 kb on + strand, within Synpcc7942_1125 at 1143.611 kb on + strand, within Synpcc7942_1125 at 1143.611 kb on + strand, within Synpcc7942_1125 at 1143.611 kb on + strand, within Synpcc7942_1125 at 1143.619 kb on - strand at 1143.626 kb on - strand, within Synpcc7942_1125 at 1143.641 kb on - strand, within Synpcc7942_1125 at 1143.642 kb on + strand, within Synpcc7942_1125 at 1143.658 kb on + strand, within Synpcc7942_1125 at 1143.724 kb on - strand, within Synpcc7942_1125 at 1143.726 kb on + strand, within Synpcc7942_1125 at 1143.727 kb on - strand, within Synpcc7942_1125 at 1143.766 kb on + strand, within Synpcc7942_1125 at 1143.792 kb on - strand, within Synpcc7942_1125 at 1143.808 kb on + strand, within Synpcc7942_1125 at 1143.808 kb on + strand, within Synpcc7942_1125 at 1143.829 kb on - strand, within Synpcc7942_1125 at 1143.829 kb on - strand, within Synpcc7942_1125 at 1143.859 kb on - strand, within Synpcc7942_1125 at 1143.936 kb on + strand, within Synpcc7942_1125 at 1143.956 kb on - strand, within Synpcc7942_1125 at 1143.986 kb on + strand, within Synpcc7942_1125 at 1143.994 kb on + strand, within Synpcc7942_1125 at 1144.004 kb on - strand, within Synpcc7942_1125 at 1144.015 kb on - strand, within Synpcc7942_1125 at 1144.016 kb on + strand, within Synpcc7942_1125 at 1144.043 kb on + strand, within Synpcc7942_1125 at 1144.051 kb on - strand, within Synpcc7942_1125 at 1144.106 kb on + strand, within Synpcc7942_1125 at 1144.114 kb on - strand, within Synpcc7942_1125 at 1144.119 kb on - strand, within Synpcc7942_1125 at 1144.171 kb on - strand, within Synpcc7942_1125 at 1144.176 kb on - strand, within Synpcc7942_1125 at 1144.176 kb on - strand, within Synpcc7942_1125 at 1144.185 kb on - strand, within Synpcc7942_1125 at 1144.198 kb on + strand, within Synpcc7942_1125 at 1144.254 kb on + strand at 1144.254 kb on + strand at 1144.257 kb on - strand at 1144.284 kb on + strand at 1144.286 kb on + strand at 1144.286 kb on + strand at 1144.290 kb on - strand at 1144.308 kb on + strand at 1144.308 kb on + strand at 1144.311 kb on - strand at 1144.316 kb on - strand at 1144.327 kb on + strand at 1144.327 kb on + strand at 1144.328 kb on + strand at 1144.335 kb on - strand at 1144.335 kb on - strand at 1144.366 kb on + strand at 1144.374 kb on - strand at 1144.374 kb on - strand at 1144.390 kb on + strand at 1144.512 kb on + strand, within Synpcc7942_1126 at 1144.528 kb on - strand, within Synpcc7942_1126 at 1144.585 kb on + strand, within Synpcc7942_1126 at 1144.611 kb on + strand, within Synpcc7942_1126 at 1144.619 kb on - strand, within Synpcc7942_1126 at 1144.891 kb on - strand, within Synpcc7942_1126 at 1144.904 kb on - strand, within Synpcc7942_1126 at 1144.940 kb on + strand, within Synpcc7942_1126 at 1144.985 kb on - strand, within Synpcc7942_1126 at 1145.002 kb on + strand, within Synpcc7942_1126 at 1145.051 kb on + strand, within Synpcc7942_1126 at 1145.051 kb on + strand, within Synpcc7942_1126 at 1145.082 kb on - strand, within Synpcc7942_1126 at 1145.220 kb on + strand at 1145.242 kb on + strand at 1145.274 kb on + strand at 1145.274 kb on + strand at 1145.282 kb on - strand at 1145.287 kb on - strand at 1145.313 kb on + strand at 1145.313 kb on + strand at 1145.335 kb on + strand at 1145.335 kb on + strand at 1145.335 kb on + strand at 1145.343 kb on - strand at 1145.343 kb on - strand at 1145.345 kb on + strand at 1145.359 kb on + strand at 1145.377 kb on + strand at 1145.419 kb on - strand at 1145.472 kb on + strand at 1145.479 kb on + strand at 1145.534 kb on + strand, within tldD at 1145.580 kb on - strand, within tldD at 1145.585 kb on + strand, within tldD at 1145.596 kb on + strand, within tldD at 1145.596 kb on + strand, within tldD at 1145.604 kb on - strand, within tldD at 1145.644 kb on + strand, within tldD at 1145.652 kb on - strand, within tldD at 1145.659 kb on + strand, within tldD at 1145.664 kb on - strand, within tldD at 1145.672 kb on - strand, within tldD at 1145.688 kb on + strand, within tldD at 1145.696 kb on - strand, within tldD at 1145.696 kb on - strand, within tldD at 1145.717 kb on - strand, within tldD at 1145.721 kb on + strand, within tldD at 1145.728 kb on + strand, within tldD at 1145.746 kb on + strand, within tldD at 1145.754 kb on - strand, within tldD at 1145.765 kb on - strand, within tldD at 1145.794 kb on + strand, within tldD at 1145.812 kb on - strand, within tldD at 1145.820 kb on - strand, within tldD at 1145.833 kb on - strand, within tldD at 1145.840 kb on - strand, within tldD at 1145.861 kb on - strand, within tldD at 1145.911 kb on + strand, within tldD at 1145.911 kb on + strand, within tldD at 1145.911 kb on + strand, within tldD at 1145.919 kb on - strand, within tldD at 1145.945 kb on - strand, within tldD at 1145.987 kb on - strand, within tldD at 1146.030 kb on + strand, within tldD at 1146.062 kb on - strand, within tldD at 1146.096 kb on - strand, within tldD at 1146.140 kb on + strand, within tldD at 1146.198 kb on + strand, within tldD
Per-strain Table
Position Strand Gene LocusTag Fraction Darkness for 120h then Flask growth in 60 uE constant light remove 1,143,463 + +0.2 1,143,463 + -0.8 1,143,471 - -0.0 1,143,472 + +0.3 1,143,480 - +0.3 1,143,542 + Synpcc7942_1125 0.15 +1.7 1,143,542 + Synpcc7942_1125 0.15 +1.0 1,143,544 + Synpcc7942_1125 0.16 +0.3 1,143,544 + Synpcc7942_1125 0.16 -0.7 1,143,544 + Synpcc7942_1125 0.16 -0.1 1,143,544 + Synpcc7942_1125 0.16 +0.0 1,143,550 - Synpcc7942_1125 0.16 +0.4 1,143,550 - Synpcc7942_1125 0.16 -1.1 1,143,550 - Synpcc7942_1125 0.16 -1.5 1,143,552 - Synpcc7942_1125 0.17 -0.2 1,143,552 - Synpcc7942_1125 0.17 +0.2 1,143,552 - Synpcc7942_1125 0.17 -0.2 1,143,560 - Synpcc7942_1125 0.17 -0.8 1,143,564 - Synpcc7942_1125 0.18 +0.3 1,143,568 + Synpcc7942_1125 0.18 +0.7 1,143,611 + Synpcc7942_1125 0.23 -0.7 1,143,611 + Synpcc7942_1125 0.23 +0.1 1,143,611 + Synpcc7942_1125 0.23 +0.2 1,143,611 + Synpcc7942_1125 0.23 +0.1 1,143,611 + Synpcc7942_1125 0.23 +0.6 1,143,619 - -1.4 1,143,626 - Synpcc7942_1125 0.24 -0.1 1,143,641 - Synpcc7942_1125 0.26 -0.8 1,143,642 + Synpcc7942_1125 0.26 +0.4 1,143,658 + Synpcc7942_1125 0.28 -0.5 1,143,724 - Synpcc7942_1125 0.35 -0.0 1,143,726 + Synpcc7942_1125 0.35 +1.0 1,143,727 - Synpcc7942_1125 0.35 -0.8 1,143,766 + Synpcc7942_1125 0.39 +0.4 1,143,792 - Synpcc7942_1125 0.42 +0.8 1,143,808 + Synpcc7942_1125 0.44 -0.7 1,143,808 + Synpcc7942_1125 0.44 -0.3 1,143,829 - Synpcc7942_1125 0.46 -1.3 1,143,829 - Synpcc7942_1125 0.46 +0.6 1,143,859 - Synpcc7942_1125 0.49 -1.7 1,143,936 + Synpcc7942_1125 0.58 +0.6 1,143,956 - Synpcc7942_1125 0.60 +0.6 1,143,986 + Synpcc7942_1125 0.63 +0.2 1,143,994 + Synpcc7942_1125 0.64 -0.5 1,144,004 - Synpcc7942_1125 0.65 -0.2 1,144,015 - Synpcc7942_1125 0.66 -0.0 1,144,016 + Synpcc7942_1125 0.66 -0.2 1,144,043 + Synpcc7942_1125 0.69 -0.1 1,144,051 - Synpcc7942_1125 0.70 +0.0 1,144,106 + Synpcc7942_1125 0.76 +0.1 1,144,114 - Synpcc7942_1125 0.77 +0.4 1,144,119 - Synpcc7942_1125 0.77 +0.3 1,144,171 - Synpcc7942_1125 0.83 +0.3 1,144,176 - Synpcc7942_1125 0.83 -1.4 1,144,176 - Synpcc7942_1125 0.83 -0.2 1,144,185 - Synpcc7942_1125 0.84 -2.1 1,144,198 + Synpcc7942_1125 0.86 -0.3 1,144,254 + -0.3 1,144,254 + -0.3 1,144,257 - -0.1 1,144,284 + +0.4 1,144,286 + -0.6 1,144,286 + +0.1 1,144,290 - -0.5 1,144,308 + +0.8 1,144,308 + -0.1 1,144,311 - -0.2 1,144,316 - -0.2 1,144,327 + -0.1 1,144,327 + +0.4 1,144,328 + -0.0 1,144,335 - +0.1 1,144,335 - -0.4 1,144,366 + +0.2 1,144,374 - +0.4 1,144,374 - -0.6 1,144,390 + -0.6 1,144,512 + Synpcc7942_1126 0.15 -2.0 1,144,528 - Synpcc7942_1126 0.17 +0.1 1,144,585 + Synpcc7942_1126 0.23 -2.0 1,144,611 + Synpcc7942_1126 0.26 -1.4 1,144,619 - Synpcc7942_1126 0.27 -1.2 1,144,891 - Synpcc7942_1126 0.60 -2.7 1,144,904 - Synpcc7942_1126 0.62 -0.4 1,144,940 + Synpcc7942_1126 0.66 -0.6 1,144,985 - Synpcc7942_1126 0.71 +0.6 1,145,002 + Synpcc7942_1126 0.73 +0.0 1,145,051 + Synpcc7942_1126 0.79 -0.8 1,145,051 + Synpcc7942_1126 0.79 -0.6 1,145,082 - Synpcc7942_1126 0.83 +0.6 1,145,220 + -0.1 1,145,242 + +0.1 1,145,274 + -0.3 1,145,274 + -0.1 1,145,282 - -0.5 1,145,287 - +0.3 1,145,313 + +0.2 1,145,313 + -0.5 1,145,335 + -0.1 1,145,335 + -0.3 1,145,335 + -0.4 1,145,343 - +0.5 1,145,343 - -0.2 1,145,345 + -0.5 1,145,359 + -0.9 1,145,377 + -0.7 1,145,419 - +0.1 1,145,472 + -2.1 1,145,479 + -0.2 1,145,534 + tldD Synpcc7942_1127 0.11 -0.1 1,145,580 - tldD Synpcc7942_1127 0.14 -0.1 1,145,585 + tldD Synpcc7942_1127 0.14 -1.4 1,145,596 + tldD Synpcc7942_1127 0.15 -0.9 1,145,596 + tldD Synpcc7942_1127 0.15 -1.1 1,145,604 - tldD Synpcc7942_1127 0.16 -0.6 1,145,644 + tldD Synpcc7942_1127 0.18 +1.3 1,145,652 - tldD Synpcc7942_1127 0.19 -0.6 1,145,659 + tldD Synpcc7942_1127 0.19 +1.3 1,145,664 - tldD Synpcc7942_1127 0.20 -0.5 1,145,672 - tldD Synpcc7942_1127 0.20 -0.1 1,145,688 + tldD Synpcc7942_1127 0.21 -0.1 1,145,696 - tldD Synpcc7942_1127 0.22 +0.2 1,145,696 - tldD Synpcc7942_1127 0.22 -0.8 1,145,717 - tldD Synpcc7942_1127 0.23 -0.2 1,145,721 + tldD Synpcc7942_1127 0.24 -1.1 1,145,728 + tldD Synpcc7942_1127 0.24 +0.3 1,145,746 + tldD Synpcc7942_1127 0.25 -1.4 1,145,754 - tldD Synpcc7942_1127 0.26 -1.0 1,145,765 - tldD Synpcc7942_1127 0.27 +0.0 1,145,794 + tldD Synpcc7942_1127 0.29 -0.8 1,145,812 - tldD Synpcc7942_1127 0.30 +1.2 1,145,820 - tldD Synpcc7942_1127 0.30 -0.8 1,145,833 - tldD Synpcc7942_1127 0.31 -0.5 1,145,840 - tldD Synpcc7942_1127 0.32 -0.1 1,145,861 - tldD Synpcc7942_1127 0.33 +0.9 1,145,911 + tldD Synpcc7942_1127 0.37 -0.3 1,145,911 + tldD Synpcc7942_1127 0.37 +0.0 1,145,911 + tldD Synpcc7942_1127 0.37 +0.6 1,145,919 - tldD Synpcc7942_1127 0.37 +0.0 1,145,945 - tldD Synpcc7942_1127 0.39 -1.0 1,145,987 - tldD Synpcc7942_1127 0.42 -0.1 1,146,030 + tldD Synpcc7942_1127 0.45 -0.8 1,146,062 - tldD Synpcc7942_1127 0.47 +0.6 1,146,096 - tldD Synpcc7942_1127 0.49 -0.4 1,146,140 + tldD Synpcc7942_1127 0.52 -1.5 1,146,198 + tldD Synpcc7942_1127 0.56 +0.1
Or see this region's nucleotide sequence