Experiment: L-Histidine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3798 and Pf6N2E2_3799 are separated by 136 nucleotides Pf6N2E2_3799 and Pf6N2E2_3800 are separated by 129 nucleotides
Pf6N2E2_3798 - Imidazolonepropionase (EC 3.5.2.7) (from data) , at 4,424,264 to 4,425,466
_3798
Pf6N2E2_3799 - Histidine ammonia-lyase (EC 4.3.1.3) (from data) , at 4,425,603 to 4,427,135
_3799
Pf6N2E2_3800 - Histidine ammonia-lyase (EC 4.3.1.3), at 4,427,265 to 4,428,788
_3800
Position (kb)
4425
4426
4427
4428 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 4424.611 kb on - strand, within Pf6N2E2_3798 at 4424.835 kb on + strand, within Pf6N2E2_3798 at 4424.916 kb on + strand, within Pf6N2E2_3798 at 4424.916 kb on + strand, within Pf6N2E2_3798 at 4424.916 kb on + strand, within Pf6N2E2_3798 at 4424.916 kb on + strand, within Pf6N2E2_3798 at 4424.917 kb on - strand, within Pf6N2E2_3798 at 4424.917 kb on - strand, within Pf6N2E2_3798 at 4424.938 kb on - strand, within Pf6N2E2_3798 at 4424.938 kb on - strand, within Pf6N2E2_3798 at 4424.979 kb on - strand, within Pf6N2E2_3798 at 4425.257 kb on + strand, within Pf6N2E2_3798 at 4425.257 kb on + strand, within Pf6N2E2_3798 at 4425.258 kb on - strand, within Pf6N2E2_3798 at 4425.258 kb on - strand, within Pf6N2E2_3798 at 4425.262 kb on + strand, within Pf6N2E2_3798 at 4425.263 kb on - strand, within Pf6N2E2_3798 at 4425.537 kb on + strand at 4425.604 kb on + strand at 4425.604 kb on + strand at 4425.605 kb on - strand at 4425.605 kb on - strand at 4425.709 kb on + strand at 4426.640 kb on + strand, within Pf6N2E2_3799 at 4426.725 kb on - strand, within Pf6N2E2_3799 at 4426.731 kb on + strand, within Pf6N2E2_3799 at 4426.731 kb on + strand, within Pf6N2E2_3799 at 4426.731 kb on + strand, within Pf6N2E2_3799 at 4426.732 kb on - strand, within Pf6N2E2_3799 at 4426.742 kb on - strand, within Pf6N2E2_3799 at 4427.078 kb on - strand at 4427.078 kb on - strand at 4427.124 kb on + strand at 4427.125 kb on - strand at 4427.125 kb on - strand at 4427.137 kb on + strand at 4427.137 kb on + strand at 4427.137 kb on + strand at 4427.137 kb on + strand at 4427.137 kb on + strand at 4427.137 kb on + strand at 4427.137 kb on + strand at 4427.137 kb on + strand at 4427.137 kb on + strand at 4427.137 kb on + strand at 4427.137 kb on + strand at 4427.137 kb on + strand at 4427.138 kb on - strand at 4427.138 kb on - strand at 4427.138 kb on - strand at 4427.138 kb on - strand at 4427.138 kb on - strand at 4427.138 kb on - strand at 4427.138 kb on - strand at 4427.138 kb on - strand at 4427.138 kb on - strand at 4427.138 kb on - strand at 4427.138 kb on - strand at 4427.138 kb on - strand at 4427.138 kb on - strand at 4427.138 kb on - strand at 4427.138 kb on - strand at 4427.225 kb on + strand at 4427.234 kb on + strand at 4427.234 kb on + strand at 4427.235 kb on - strand at 4427.235 kb on - strand at 4427.235 kb on - strand at 4427.235 kb on - strand at 4427.235 kb on - strand at 4427.235 kb on - strand at 4427.235 kb on - strand at 4427.283 kb on + strand at 4427.629 kb on + strand, within Pf6N2E2_3800 at 4427.948 kb on + strand, within Pf6N2E2_3800 at 4427.966 kb on + strand, within Pf6N2E2_3800
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (C) remove 4,424,611 - Pf6N2E2_3798 0.29 -3.1 4,424,835 + Pf6N2E2_3798 0.47 -2.9 4,424,916 + Pf6N2E2_3798 0.54 -2.4 4,424,916 + Pf6N2E2_3798 0.54 -3.0 4,424,916 + Pf6N2E2_3798 0.54 -4.4 4,424,916 + Pf6N2E2_3798 0.54 -3.3 4,424,917 - Pf6N2E2_3798 0.54 -3.2 4,424,917 - Pf6N2E2_3798 0.54 -0.5 4,424,938 - Pf6N2E2_3798 0.56 -3.8 4,424,938 - Pf6N2E2_3798 0.56 -3.6 4,424,979 - Pf6N2E2_3798 0.59 -2.9 4,425,257 + Pf6N2E2_3798 0.83 -4.0 4,425,257 + Pf6N2E2_3798 0.83 -2.9 4,425,258 - Pf6N2E2_3798 0.83 -2.9 4,425,258 - Pf6N2E2_3798 0.83 -4.1 4,425,262 + Pf6N2E2_3798 0.83 -3.2 4,425,263 - Pf6N2E2_3798 0.83 -2.6 4,425,537 + -3.4 4,425,604 + -1.8 4,425,604 + -4.3 4,425,605 - +0.0 4,425,605 - -1.6 4,425,709 + -3.7 4,426,640 + Pf6N2E2_3799 0.68 -3.6 4,426,725 - Pf6N2E2_3799 0.73 -0.1 4,426,731 + Pf6N2E2_3799 0.74 -5.3 4,426,731 + Pf6N2E2_3799 0.74 -3.0 4,426,731 + Pf6N2E2_3799 0.74 -3.6 4,426,732 - Pf6N2E2_3799 0.74 -3.2 4,426,742 - Pf6N2E2_3799 0.74 -3.2 4,427,078 - -4.3 4,427,078 - -4.1 4,427,124 + -2.6 4,427,125 - -3.4 4,427,125 - -3.2 4,427,137 + -3.0 4,427,137 + -2.7 4,427,137 + -1.9 4,427,137 + -2.3 4,427,137 + -3.6 4,427,137 + -3.8 4,427,137 + -2.1 4,427,137 + -3.3 4,427,137 + -3.9 4,427,137 + -4.6 4,427,137 + -2.2 4,427,137 + -1.6 4,427,138 - -1.8 4,427,138 - -0.4 4,427,138 - -1.7 4,427,138 - -3.6 4,427,138 - -3.1 4,427,138 - -2.6 4,427,138 - -2.8 4,427,138 - -3.5 4,427,138 - -4.8 4,427,138 - -3.4 4,427,138 - -3.6 4,427,138 - -2.6 4,427,138 - -2.0 4,427,138 - -2.3 4,427,138 - -2.7 4,427,225 + -4.4 4,427,234 + -4.1 4,427,234 + -1.3 4,427,235 - -2.2 4,427,235 - -1.9 4,427,235 - +0.7 4,427,235 - -2.2 4,427,235 - -0.9 4,427,235 - +1.0 4,427,235 - +0.9 4,427,283 + -3.4 4,427,629 + Pf6N2E2_3800 0.24 -0.7 4,427,948 + Pf6N2E2_3800 0.45 +0.5 4,427,966 + Pf6N2E2_3800 0.46 -4.1
Or see this region's nucleotide sequence