Experiment: L-Histidine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3343 and Pf6N2E2_3344 overlap by 4 nucleotides Pf6N2E2_3344 and Pf6N2E2_3345 are separated by 64 nucleotides
Pf6N2E2_3343 - AmpE protein, at 3,870,841 to 3,871,677
_3343
Pf6N2E2_3344 - N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28) AmpD, at 3,871,674 to 3,872,234
_3344
Pf6N2E2_3345 - Thymidine phosphorylase (EC 2.4.2.4), at 3,872,299 to 3,874,512
_3345
Position (kb)
3871
3872
3873 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3870.690 kb on + strand at 3870.690 kb on + strand at 3870.690 kb on + strand at 3870.690 kb on + strand at 3870.690 kb on + strand at 3870.691 kb on - strand at 3870.691 kb on - strand at 3870.691 kb on - strand at 3870.691 kb on - strand at 3870.875 kb on + strand at 3870.991 kb on - strand, within Pf6N2E2_3343 at 3871.008 kb on + strand, within Pf6N2E2_3343 at 3871.009 kb on - strand, within Pf6N2E2_3343 at 3871.009 kb on - strand, within Pf6N2E2_3343 at 3871.250 kb on + strand, within Pf6N2E2_3343 at 3871.251 kb on - strand, within Pf6N2E2_3343 at 3871.421 kb on + strand, within Pf6N2E2_3343 at 3871.421 kb on + strand, within Pf6N2E2_3343 at 3871.464 kb on - strand, within Pf6N2E2_3343 at 3871.464 kb on - strand, within Pf6N2E2_3343 at 3871.778 kb on + strand, within Pf6N2E2_3344 at 3871.779 kb on - strand, within Pf6N2E2_3344 at 3871.843 kb on + strand, within Pf6N2E2_3344 at 3871.844 kb on - strand, within Pf6N2E2_3344 at 3871.844 kb on - strand, within Pf6N2E2_3344 at 3871.844 kb on - strand, within Pf6N2E2_3344 at 3871.907 kb on + strand, within Pf6N2E2_3344 at 3871.908 kb on - strand, within Pf6N2E2_3344 at 3871.908 kb on - strand, within Pf6N2E2_3344 at 3872.045 kb on - strand, within Pf6N2E2_3344 at 3872.126 kb on + strand, within Pf6N2E2_3344 at 3872.234 kb on + strand at 3872.235 kb on - strand at 3872.271 kb on + strand at 3872.272 kb on - strand at 3872.272 kb on - strand at 3872.465 kb on + strand at 3872.465 kb on + strand at 3872.465 kb on + strand at 3872.465 kb on + strand at 3872.466 kb on - strand at 3872.466 kb on - strand at 3872.466 kb on - strand at 3872.830 kb on + strand, within Pf6N2E2_3345 at 3872.931 kb on - strand, within Pf6N2E2_3345 at 3873.205 kb on + strand, within Pf6N2E2_3345
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (C) remove 3,870,690 + -0.3 3,870,690 + +0.2 3,870,690 + +0.2 3,870,690 + -0.6 3,870,690 + -1.0 3,870,691 - -0.4 3,870,691 - +0.6 3,870,691 - +0.0 3,870,691 - +0.0 3,870,875 + -0.2 3,870,991 - Pf6N2E2_3343 0.18 -0.4 3,871,008 + Pf6N2E2_3343 0.20 +0.1 3,871,009 - Pf6N2E2_3343 0.20 -0.5 3,871,009 - Pf6N2E2_3343 0.20 -0.2 3,871,250 + Pf6N2E2_3343 0.49 +0.7 3,871,251 - Pf6N2E2_3343 0.49 -0.3 3,871,421 + Pf6N2E2_3343 0.69 -0.4 3,871,421 + Pf6N2E2_3343 0.69 +0.3 3,871,464 - Pf6N2E2_3343 0.74 +0.3 3,871,464 - Pf6N2E2_3343 0.74 -0.3 3,871,778 + Pf6N2E2_3344 0.19 +0.3 3,871,779 - Pf6N2E2_3344 0.19 -0.5 3,871,843 + Pf6N2E2_3344 0.30 -0.2 3,871,844 - Pf6N2E2_3344 0.30 -0.8 3,871,844 - Pf6N2E2_3344 0.30 -1.8 3,871,844 - Pf6N2E2_3344 0.30 -1.3 3,871,907 + Pf6N2E2_3344 0.42 -0.9 3,871,908 - Pf6N2E2_3344 0.42 -1.0 3,871,908 - Pf6N2E2_3344 0.42 -1.0 3,872,045 - Pf6N2E2_3344 0.66 -3.0 3,872,126 + Pf6N2E2_3344 0.81 -0.4 3,872,234 + -0.5 3,872,235 - +1.2 3,872,271 + +1.3 3,872,272 - -1.2 3,872,272 - -0.0 3,872,465 + -0.4 3,872,465 + +0.3 3,872,465 + +0.4 3,872,465 + +0.6 3,872,466 - -0.5 3,872,466 - -0.8 3,872,466 - +0.2 3,872,830 + Pf6N2E2_3345 0.24 +0.1 3,872,931 - Pf6N2E2_3345 0.29 -1.1 3,873,205 + Pf6N2E2_3345 0.41 +0.5
Or see this region's nucleotide sequence