Experiment: L-Histidine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3191 and Pf6N2E2_3192 are separated by 1 nucleotides Pf6N2E2_3192 and Pf6N2E2_3193 are separated by 144 nucleotides
Pf6N2E2_3191 - Serine acetyltransferase (EC 2.3.1.30), at 3,684,848 to 3,685,624
_3191
Pf6N2E2_3192 - tRNA:Cm32/Um32 methyltransferase, at 3,685,626 to 3,686,396
_3192
Pf6N2E2_3193 - Inositol-1-monophosphatase (EC 3.1.3.25), at 3,686,541 to 3,687,359
_3193
Position (kb)
3685
3686
3687 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3684.697 kb on + strand at 3684.780 kb on + strand at 3684.780 kb on + strand at 3684.781 kb on - strand at 3684.781 kb on - strand at 3684.795 kb on + strand at 3684.849 kb on + strand at 3684.927 kb on + strand, within Pf6N2E2_3191 at 3684.928 kb on - strand, within Pf6N2E2_3191 at 3684.928 kb on - strand, within Pf6N2E2_3191 at 3684.931 kb on + strand, within Pf6N2E2_3191 at 3684.931 kb on + strand, within Pf6N2E2_3191 at 3684.932 kb on - strand, within Pf6N2E2_3191 at 3684.932 kb on - strand, within Pf6N2E2_3191 at 3684.932 kb on - strand, within Pf6N2E2_3191 at 3684.932 kb on - strand, within Pf6N2E2_3191 at 3685.054 kb on - strand, within Pf6N2E2_3191 at 3685.641 kb on - strand at 3685.693 kb on - strand at 3685.693 kb on - strand at 3685.694 kb on + strand at 3685.694 kb on + strand at 3685.694 kb on + strand at 3685.695 kb on - strand at 3685.695 kb on - strand at 3685.695 kb on - strand at 3685.695 kb on - strand at 3685.695 kb on - strand at 3685.695 kb on - strand at 3685.695 kb on - strand at 3685.695 kb on - strand at 3685.695 kb on - strand at 3685.695 kb on - strand at 3685.730 kb on - strand, within Pf6N2E2_3192 at 3685.816 kb on + strand, within Pf6N2E2_3192 at 3685.817 kb on - strand, within Pf6N2E2_3192 at 3685.817 kb on - strand, within Pf6N2E2_3192 at 3685.817 kb on - strand, within Pf6N2E2_3192 at 3685.933 kb on + strand, within Pf6N2E2_3192 at 3685.934 kb on - strand, within Pf6N2E2_3192 at 3685.934 kb on - strand, within Pf6N2E2_3192 at 3685.934 kb on - strand, within Pf6N2E2_3192 at 3686.141 kb on + strand, within Pf6N2E2_3192 at 3686.364 kb on - strand at 3686.418 kb on + strand at 3686.446 kb on + strand at 3686.446 kb on + strand at 3686.457 kb on + strand at 3687.358 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (C) remove 3,684,697 + +0.6 3,684,780 + +0.6 3,684,780 + +0.9 3,684,781 - -0.4 3,684,781 - +0.5 3,684,795 + +0.6 3,684,849 + +0.4 3,684,927 + Pf6N2E2_3191 0.10 -1.9 3,684,928 - Pf6N2E2_3191 0.10 -0.2 3,684,928 - Pf6N2E2_3191 0.10 -0.1 3,684,931 + Pf6N2E2_3191 0.11 -0.8 3,684,931 + Pf6N2E2_3191 0.11 +0.3 3,684,932 - Pf6N2E2_3191 0.11 -0.3 3,684,932 - Pf6N2E2_3191 0.11 +0.1 3,684,932 - Pf6N2E2_3191 0.11 -0.0 3,684,932 - Pf6N2E2_3191 0.11 -0.2 3,685,054 - Pf6N2E2_3191 0.27 +1.3 3,685,641 - -0.8 3,685,693 - +0.1 3,685,693 - +0.3 3,685,694 + -0.8 3,685,694 + -0.8 3,685,694 + +1.9 3,685,695 - -1.1 3,685,695 - -0.1 3,685,695 - +0.3 3,685,695 - -0.5 3,685,695 - -0.9 3,685,695 - -0.7 3,685,695 - -0.8 3,685,695 - +0.1 3,685,695 - -1.9 3,685,695 - -0.9 3,685,730 - Pf6N2E2_3192 0.13 -0.9 3,685,816 + Pf6N2E2_3192 0.25 +0.4 3,685,817 - Pf6N2E2_3192 0.25 -1.5 3,685,817 - Pf6N2E2_3192 0.25 -2.2 3,685,817 - Pf6N2E2_3192 0.25 -0.8 3,685,933 + Pf6N2E2_3192 0.40 +0.5 3,685,934 - Pf6N2E2_3192 0.40 -0.6 3,685,934 - Pf6N2E2_3192 0.40 -2.5 3,685,934 - Pf6N2E2_3192 0.40 -1.3 3,686,141 + Pf6N2E2_3192 0.67 -0.3 3,686,364 - -1.3 3,686,418 + +0.3 3,686,446 + -0.3 3,686,446 + +0.4 3,686,457 + +0.5 3,687,358 - +0.7
Or see this region's nucleotide sequence