Experiment: L-Histidine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1816 and Pf6N2E2_1817 overlap by 4 nucleotides Pf6N2E2_1817 and Pf6N2E2_1818 are separated by 46 nucleotides Pf6N2E2_1818 and Pf6N2E2_1819 are separated by 137 nucleotides
Pf6N2E2_1816 - Pyruvate dehydrogenase E1 component (EC 1.2.4.1), at 2,148,793 to 2,151,489
_1816
Pf6N2E2_1817 - hypothetical protein, at 2,151,486 to 2,151,908
_1817
Pf6N2E2_1818 - hypothetical protein, at 2,151,955 to 2,152,413
_1818
Pf6N2E2_1819 - 1-aminocyclopropane-1-carboxylate deaminase (EC 3.5.99.7), at 2,152,551 to 2,153,567
_1819
Position (kb)
2151
2152 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2150.850 kb on - strand, within Pf6N2E2_1816 at 2150.850 kb on - strand, within Pf6N2E2_1816 at 2151.030 kb on - strand, within Pf6N2E2_1816 at 2151.030 kb on - strand, within Pf6N2E2_1816 at 2151.056 kb on + strand, within Pf6N2E2_1816 at 2151.057 kb on - strand, within Pf6N2E2_1816 at 2151.057 kb on - strand, within Pf6N2E2_1816 at 2151.143 kb on + strand at 2151.144 kb on - strand, within Pf6N2E2_1816 at 2151.144 kb on - strand, within Pf6N2E2_1816 at 2151.288 kb on + strand at 2151.297 kb on - strand at 2151.297 kb on - strand at 2151.489 kb on + strand at 2151.490 kb on - strand at 2151.559 kb on - strand, within Pf6N2E2_1817 at 2151.559 kb on - strand, within Pf6N2E2_1817 at 2151.559 kb on - strand, within Pf6N2E2_1817 at 2151.568 kb on - strand, within Pf6N2E2_1817 at 2151.845 kb on + strand, within Pf6N2E2_1817 at 2151.909 kb on - strand at 2152.181 kb on + strand, within Pf6N2E2_1818 at 2152.182 kb on - strand, within Pf6N2E2_1818 at 2152.271 kb on + strand, within Pf6N2E2_1818 at 2152.294 kb on + strand, within Pf6N2E2_1818 at 2152.295 kb on - strand, within Pf6N2E2_1818 at 2152.322 kb on + strand, within Pf6N2E2_1818 at 2152.322 kb on + strand, within Pf6N2E2_1818 at 2152.322 kb on + strand, within Pf6N2E2_1818 at 2152.323 kb on - strand, within Pf6N2E2_1818 at 2152.577 kb on - strand at 2152.687 kb on + strand, within Pf6N2E2_1819 at 2152.845 kb on - strand, within Pf6N2E2_1819
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (C) remove 2,150,850 - Pf6N2E2_1816 0.76 -0.3 2,150,850 - Pf6N2E2_1816 0.76 +0.1 2,151,030 - Pf6N2E2_1816 0.83 +1.4 2,151,030 - Pf6N2E2_1816 0.83 -0.9 2,151,056 + Pf6N2E2_1816 0.84 +0.0 2,151,057 - Pf6N2E2_1816 0.84 -0.1 2,151,057 - Pf6N2E2_1816 0.84 +2.9 2,151,143 + -0.4 2,151,144 - Pf6N2E2_1816 0.87 +1.1 2,151,144 - Pf6N2E2_1816 0.87 -0.1 2,151,288 + +0.5 2,151,297 - -0.7 2,151,297 - -0.7 2,151,489 + +0.3 2,151,490 - -0.9 2,151,559 - Pf6N2E2_1817 0.17 +1.1 2,151,559 - Pf6N2E2_1817 0.17 -1.1 2,151,559 - Pf6N2E2_1817 0.17 +1.1 2,151,568 - Pf6N2E2_1817 0.19 +1.6 2,151,845 + Pf6N2E2_1817 0.85 +1.0 2,151,909 - +0.2 2,152,181 + Pf6N2E2_1818 0.49 +0.2 2,152,182 - Pf6N2E2_1818 0.49 -0.5 2,152,271 + Pf6N2E2_1818 0.69 -1.0 2,152,294 + Pf6N2E2_1818 0.74 +1.4 2,152,295 - Pf6N2E2_1818 0.74 -0.1 2,152,322 + Pf6N2E2_1818 0.80 +1.4 2,152,322 + Pf6N2E2_1818 0.80 -0.3 2,152,322 + Pf6N2E2_1818 0.80 -0.1 2,152,323 - Pf6N2E2_1818 0.80 -0.2 2,152,577 - +0.1 2,152,687 + Pf6N2E2_1819 0.13 -0.1 2,152,845 - Pf6N2E2_1819 0.29 +0.5
Or see this region's nucleotide sequence