Experiment: L-Histidine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1386 and Pf6N2E2_1387 are separated by 5 nucleotides Pf6N2E2_1387 and Pf6N2E2_1388 overlap by 78 nucleotides Pf6N2E2_1388 and Pf6N2E2_1389 are separated by 137 nucleotides Pf6N2E2_1389 and Pf6N2E2_1390 are separated by 147 nucleotides Pf6N2E2_1390 and Pf6N2E2_1391 are separated by 165 nucleotides
Pf6N2E2_1386 - amino acid ABC transporter, periplasmic substrate-binding protein, at 1,627,779 to 1,628,654
_1386
Pf6N2E2_1387 - Cytochrome C550 (Soluble cytochrome C), at 1,628,660 to 1,629,121
_1387
Pf6N2E2_1388 - hypothetical protein, at 1,629,044 to 1,629,238
_1388
Pf6N2E2_1389 - Quino(hemo)protein alcohol dehydrogenase, PQQ-dependent (EC 1.1.99.8), at 1,629,376 to 1,631,232
_1389
Pf6N2E2_1390 - pentapeptide repeat family protein, at 1,631,380 to 1,632,024
_1390
Pf6N2E2_1391 - DNA-binding response regulator, LuxR family, at 1,632,190 to 1,632,828
_1391
Position (kb)
1629
1630
1631
1632 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5
6
7 at 1628.386 kb on + strand, within Pf6N2E2_1386 at 1628.387 kb on - strand, within Pf6N2E2_1386 at 1628.387 kb on - strand, within Pf6N2E2_1386 at 1628.387 kb on - strand, within Pf6N2E2_1386 at 1628.387 kb on - strand, within Pf6N2E2_1386 at 1628.521 kb on + strand, within Pf6N2E2_1386 at 1628.572 kb on - strand at 1628.581 kb on + strand at 1628.582 kb on - strand at 1628.676 kb on - strand at 1628.724 kb on + strand, within Pf6N2E2_1387 at 1628.724 kb on + strand, within Pf6N2E2_1387 at 1628.724 kb on + strand, within Pf6N2E2_1387 at 1628.745 kb on + strand, within Pf6N2E2_1387 at 1628.745 kb on + strand, within Pf6N2E2_1387 at 1628.745 kb on + strand, within Pf6N2E2_1387 at 1628.746 kb on - strand, within Pf6N2E2_1387 at 1628.746 kb on - strand, within Pf6N2E2_1387 at 1628.746 kb on - strand, within Pf6N2E2_1387 at 1628.746 kb on - strand, within Pf6N2E2_1387 at 1628.777 kb on + strand, within Pf6N2E2_1387 at 1628.777 kb on + strand, within Pf6N2E2_1387 at 1628.777 kb on + strand, within Pf6N2E2_1387 at 1629.035 kb on + strand, within Pf6N2E2_1387 at 1629.035 kb on + strand, within Pf6N2E2_1387 at 1629.036 kb on - strand, within Pf6N2E2_1387 at 1629.179 kb on - strand, within Pf6N2E2_1388 at 1629.179 kb on - strand, within Pf6N2E2_1388 at 1629.225 kb on + strand at 1629.225 kb on + strand at 1629.225 kb on + strand at 1629.225 kb on + strand at 1629.226 kb on - strand at 1629.226 kb on - strand at 1629.236 kb on + strand at 1629.237 kb on - strand at 1629.261 kb on - strand at 1629.611 kb on - strand, within Pf6N2E2_1389 at 1629.611 kb on - strand, within Pf6N2E2_1389 at 1629.676 kb on + strand, within Pf6N2E2_1389 at 1629.676 kb on + strand, within Pf6N2E2_1389 at 1629.677 kb on - strand, within Pf6N2E2_1389 at 1629.677 kb on - strand, within Pf6N2E2_1389 at 1629.742 kb on + strand, within Pf6N2E2_1389 at 1629.743 kb on - strand, within Pf6N2E2_1389 at 1629.808 kb on + strand, within Pf6N2E2_1389 at 1629.808 kb on + strand, within Pf6N2E2_1389 at 1629.809 kb on - strand, within Pf6N2E2_1389 at 1629.823 kb on + strand, within Pf6N2E2_1389 at 1629.823 kb on + strand, within Pf6N2E2_1389 at 1629.824 kb on - strand, within Pf6N2E2_1389 at 1629.824 kb on - strand, within Pf6N2E2_1389 at 1629.861 kb on + strand, within Pf6N2E2_1389 at 1630.316 kb on - strand, within Pf6N2E2_1389 at 1630.327 kb on + strand, within Pf6N2E2_1389 at 1630.327 kb on + strand, within Pf6N2E2_1389 at 1630.328 kb on - strand, within Pf6N2E2_1389 at 1630.381 kb on + strand at 1630.382 kb on - strand, within Pf6N2E2_1389 at 1630.382 kb on - strand, within Pf6N2E2_1389 at 1630.382 kb on - strand, within Pf6N2E2_1389 at 1630.445 kb on - strand, within Pf6N2E2_1389 at 1630.507 kb on + strand, within Pf6N2E2_1389 at 1630.508 kb on - strand, within Pf6N2E2_1389 at 1630.508 kb on - strand, within Pf6N2E2_1389 at 1630.508 kb on - strand, within Pf6N2E2_1389 at 1630.717 kb on + strand, within Pf6N2E2_1389 at 1630.717 kb on + strand, within Pf6N2E2_1389 at 1630.718 kb on - strand, within Pf6N2E2_1389 at 1630.718 kb on - strand, within Pf6N2E2_1389 at 1630.718 kb on - strand, within Pf6N2E2_1389 at 1630.741 kb on + strand, within Pf6N2E2_1389 at 1630.741 kb on + strand, within Pf6N2E2_1389 at 1630.741 kb on + strand, within Pf6N2E2_1389 at 1630.741 kb on + strand, within Pf6N2E2_1389 at 1630.741 kb on + strand, within Pf6N2E2_1389 at 1630.742 kb on - strand, within Pf6N2E2_1389 at 1630.742 kb on - strand, within Pf6N2E2_1389 at 1630.772 kb on - strand, within Pf6N2E2_1389 at 1630.793 kb on - strand, within Pf6N2E2_1389 at 1630.810 kb on + strand, within Pf6N2E2_1389 at 1630.843 kb on + strand, within Pf6N2E2_1389 at 1630.843 kb on + strand, within Pf6N2E2_1389 at 1630.843 kb on + strand, within Pf6N2E2_1389 at 1630.844 kb on - strand, within Pf6N2E2_1389 at 1630.895 kb on + strand, within Pf6N2E2_1389 at 1630.895 kb on + strand, within Pf6N2E2_1389 at 1630.895 kb on + strand, within Pf6N2E2_1389 at 1630.895 kb on + strand, within Pf6N2E2_1389 at 1630.895 kb on + strand, within Pf6N2E2_1389 at 1630.896 kb on - strand, within Pf6N2E2_1389 at 1631.230 kb on + strand at 1631.443 kb on - strand at 1632.023 kb on - strand at 1632.191 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (C) remove 1,628,386 + Pf6N2E2_1386 0.69 +2.2 1,628,387 - Pf6N2E2_1386 0.69 +1.5 1,628,387 - Pf6N2E2_1386 0.69 +1.5 1,628,387 - Pf6N2E2_1386 0.69 +1.4 1,628,387 - Pf6N2E2_1386 0.69 +2.0 1,628,521 + Pf6N2E2_1386 0.85 +2.5 1,628,572 - +1.6 1,628,581 + +2.0 1,628,582 - +2.6 1,628,676 - +4.0 1,628,724 + Pf6N2E2_1387 0.14 +3.8 1,628,724 + Pf6N2E2_1387 0.14 +4.0 1,628,724 + Pf6N2E2_1387 0.14 +3.9 1,628,745 + Pf6N2E2_1387 0.18 +4.1 1,628,745 + Pf6N2E2_1387 0.18 +3.7 1,628,745 + Pf6N2E2_1387 0.18 +4.3 1,628,746 - Pf6N2E2_1387 0.19 +3.1 1,628,746 - Pf6N2E2_1387 0.19 +4.3 1,628,746 - Pf6N2E2_1387 0.19 +4.2 1,628,746 - Pf6N2E2_1387 0.19 +4.2 1,628,777 + Pf6N2E2_1387 0.25 +3.3 1,628,777 + Pf6N2E2_1387 0.25 +4.0 1,628,777 + Pf6N2E2_1387 0.25 +3.0 1,629,035 + Pf6N2E2_1387 0.81 +5.0 1,629,035 + Pf6N2E2_1387 0.81 +4.1 1,629,036 - Pf6N2E2_1387 0.81 +3.5 1,629,179 - Pf6N2E2_1388 0.69 +2.1 1,629,179 - Pf6N2E2_1388 0.69 +3.7 1,629,225 + +5.7 1,629,225 + +5.4 1,629,225 + +5.8 1,629,225 + +5.5 1,629,226 - +5.0 1,629,226 - +4.7 1,629,236 + +6.3 1,629,237 - +5.8 1,629,261 - +7.3 1,629,611 - Pf6N2E2_1389 0.13 +5.9 1,629,611 - Pf6N2E2_1389 0.13 +6.1 1,629,676 + Pf6N2E2_1389 0.16 +6.0 1,629,676 + Pf6N2E2_1389 0.16 +6.2 1,629,677 - Pf6N2E2_1389 0.16 +6.4 1,629,677 - Pf6N2E2_1389 0.16 +5.8 1,629,742 + Pf6N2E2_1389 0.20 +6.3 1,629,743 - Pf6N2E2_1389 0.20 +5.4 1,629,808 + Pf6N2E2_1389 0.23 +6.2 1,629,808 + Pf6N2E2_1389 0.23 +6.1 1,629,809 - Pf6N2E2_1389 0.23 +5.7 1,629,823 + Pf6N2E2_1389 0.24 +6.1 1,629,823 + Pf6N2E2_1389 0.24 +6.7 1,629,824 - Pf6N2E2_1389 0.24 +5.3 1,629,824 - Pf6N2E2_1389 0.24 +6.4 1,629,861 + Pf6N2E2_1389 0.26 +7.0 1,630,316 - Pf6N2E2_1389 0.51 +6.7 1,630,327 + Pf6N2E2_1389 0.51 +6.7 1,630,327 + Pf6N2E2_1389 0.51 +6.1 1,630,328 - Pf6N2E2_1389 0.51 +7.1 1,630,381 + +6.1 1,630,382 - Pf6N2E2_1389 0.54 +6.7 1,630,382 - Pf6N2E2_1389 0.54 +5.1 1,630,382 - Pf6N2E2_1389 0.54 +7.4 1,630,445 - Pf6N2E2_1389 0.58 +6.3 1,630,507 + Pf6N2E2_1389 0.61 +6.2 1,630,508 - Pf6N2E2_1389 0.61 +6.3 1,630,508 - Pf6N2E2_1389 0.61 +5.8 1,630,508 - Pf6N2E2_1389 0.61 +6.1 1,630,717 + Pf6N2E2_1389 0.72 +6.4 1,630,717 + Pf6N2E2_1389 0.72 +5.4 1,630,718 - Pf6N2E2_1389 0.72 +5.3 1,630,718 - Pf6N2E2_1389 0.72 +5.3 1,630,718 - Pf6N2E2_1389 0.72 +6.5 1,630,741 + Pf6N2E2_1389 0.74 +5.5 1,630,741 + Pf6N2E2_1389 0.74 +5.8 1,630,741 + Pf6N2E2_1389 0.74 +5.9 1,630,741 + Pf6N2E2_1389 0.74 +6.1 1,630,741 + Pf6N2E2_1389 0.74 +5.5 1,630,742 - Pf6N2E2_1389 0.74 +6.5 1,630,742 - Pf6N2E2_1389 0.74 +5.7 1,630,772 - Pf6N2E2_1389 0.75 +5.4 1,630,793 - Pf6N2E2_1389 0.76 +7.0 1,630,810 + Pf6N2E2_1389 0.77 +4.9 1,630,843 + Pf6N2E2_1389 0.79 +6.0 1,630,843 + Pf6N2E2_1389 0.79 +6.5 1,630,843 + Pf6N2E2_1389 0.79 +5.4 1,630,844 - Pf6N2E2_1389 0.79 +6.6 1,630,895 + Pf6N2E2_1389 0.82 +5.8 1,630,895 + Pf6N2E2_1389 0.82 +5.7 1,630,895 + Pf6N2E2_1389 0.82 +5.7 1,630,895 + Pf6N2E2_1389 0.82 +6.5 1,630,895 + Pf6N2E2_1389 0.82 +5.9 1,630,896 - Pf6N2E2_1389 0.82 +5.9 1,631,230 + +6.0 1,631,443 - +3.2 1,632,023 - +0.1 1,632,191 + -0.2
Or see this region's nucleotide sequence