Experiment: Nickel (II) chloride 0.6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_44 and Ac3H11_45 overlap by 30 nucleotides Ac3H11_45 and Ac3H11_46 are separated by 127 nucleotides
Ac3H11_44 - TPR repeat precursor, at 41,082 to 42,158
_44
Ac3H11_45 - hypothetical protein, at 42,129 to 42,263
_45
Ac3H11_46 - Cysteinyl-tRNA synthetase (EC 6.1.1.16), at 42,391 to 43,764
_46
Position (kb)
42
43 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 41.179 kb on + strand at 41.179 kb on + strand at 41.179 kb on + strand at 41.179 kb on + strand at 41.179 kb on + strand at 41.180 kb on - strand at 41.180 kb on - strand at 41.180 kb on - strand at 41.180 kb on - strand at 41.180 kb on - strand at 41.180 kb on - strand at 41.338 kb on + strand, within Ac3H11_44 at 41.350 kb on + strand, within Ac3H11_44 at 41.350 kb on + strand, within Ac3H11_44 at 41.350 kb on + strand, within Ac3H11_44 at 41.351 kb on - strand, within Ac3H11_44 at 41.351 kb on - strand, within Ac3H11_44 at 41.471 kb on + strand, within Ac3H11_44 at 41.472 kb on - strand, within Ac3H11_44 at 41.719 kb on + strand, within Ac3H11_44 at 41.720 kb on - strand, within Ac3H11_44 at 41.720 kb on - strand, within Ac3H11_44 at 41.720 kb on - strand, within Ac3H11_44 at 41.720 kb on - strand, within Ac3H11_44 at 41.720 kb on - strand, within Ac3H11_44 at 41.743 kb on + strand, within Ac3H11_44 at 41.743 kb on + strand, within Ac3H11_44 at 41.743 kb on + strand, within Ac3H11_44 at 41.743 kb on + strand, within Ac3H11_44 at 41.743 kb on + strand, within Ac3H11_44 at 41.743 kb on + strand, within Ac3H11_44 at 41.744 kb on - strand, within Ac3H11_44 at 41.744 kb on - strand, within Ac3H11_44 at 41.744 kb on - strand, within Ac3H11_44 at 41.744 kb on - strand, within Ac3H11_44 at 41.744 kb on - strand, within Ac3H11_44 at 41.744 kb on - strand, within Ac3H11_44 at 41.744 kb on - strand, within Ac3H11_44 at 41.744 kb on - strand, within Ac3H11_44 at 41.776 kb on + strand, within Ac3H11_44 at 41.776 kb on + strand, within Ac3H11_44 at 41.776 kb on + strand, within Ac3H11_44 at 41.845 kb on + strand, within Ac3H11_44 at 41.846 kb on - strand, within Ac3H11_44 at 41.866 kb on + strand, within Ac3H11_44 at 41.866 kb on + strand, within Ac3H11_44 at 41.867 kb on - strand, within Ac3H11_44 at 41.867 kb on - strand, within Ac3H11_44 at 41.867 kb on - strand, within Ac3H11_44 at 41.867 kb on - strand, within Ac3H11_44 at 41.867 kb on - strand, within Ac3H11_44 at 41.867 kb on - strand, within Ac3H11_44 at 41.887 kb on + strand, within Ac3H11_44 at 42.068 kb on - strand at 42.068 kb on - strand at 42.068 kb on - strand at 42.203 kb on + strand, within Ac3H11_45 at 42.203 kb on + strand, within Ac3H11_45 at 42.203 kb on + strand, within Ac3H11_45 at 42.203 kb on + strand, within Ac3H11_45 at 42.203 kb on + strand, within Ac3H11_45 at 42.203 kb on + strand, within Ac3H11_45 at 42.203 kb on + strand, within Ac3H11_45 at 42.203 kb on + strand, within Ac3H11_45 at 42.203 kb on + strand, within Ac3H11_45 at 42.203 kb on + strand, within Ac3H11_45 at 42.203 kb on + strand, within Ac3H11_45 at 42.203 kb on + strand, within Ac3H11_45 at 42.203 kb on + strand, within Ac3H11_45 at 42.203 kb on + strand, within Ac3H11_45 at 42.204 kb on - strand, within Ac3H11_45 at 42.204 kb on - strand, within Ac3H11_45 at 42.204 kb on - strand, within Ac3H11_45 at 42.204 kb on - strand, within Ac3H11_45 at 42.204 kb on - strand, within Ac3H11_45 at 42.204 kb on - strand, within Ac3H11_45 at 42.204 kb on - strand, within Ac3H11_45 at 42.204 kb on - strand, within Ac3H11_45 at 42.204 kb on - strand, within Ac3H11_45 at 42.204 kb on - strand, within Ac3H11_45 at 42.204 kb on - strand, within Ac3H11_45 at 42.204 kb on - strand, within Ac3H11_45 at 42.204 kb on - strand, within Ac3H11_45 at 42.204 kb on - strand, within Ac3H11_45 at 42.204 kb on - strand, within Ac3H11_45 at 42.205 kb on + strand, within Ac3H11_45 at 42.205 kb on + strand, within Ac3H11_45 at 42.205 kb on + strand, within Ac3H11_45 at 42.205 kb on + strand, within Ac3H11_45 at 42.205 kb on + strand, within Ac3H11_45 at 42.205 kb on + strand, within Ac3H11_45 at 42.205 kb on + strand, within Ac3H11_45 at 42.205 kb on + strand, within Ac3H11_45 at 42.220 kb on + strand, within Ac3H11_45 at 42.220 kb on + strand, within Ac3H11_45 at 42.272 kb on + strand at 42.272 kb on + strand at 42.272 kb on + strand at 42.272 kb on + strand at 42.272 kb on + strand at 42.272 kb on + strand at 42.272 kb on + strand at 42.272 kb on + strand at 42.272 kb on + strand at 42.272 kb on + strand at 42.329 kb on + strand at 42.329 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 0.6 mM remove 41,179 + +0.4 41,179 + +0.5 41,179 + +0.4 41,179 + -2.1 41,179 + +1.0 41,180 - -0.4 41,180 - +0.4 41,180 - -0.1 41,180 - +0.2 41,180 - +0.3 41,180 - -0.6 41,338 + Ac3H11_44 0.24 +0.7 41,350 + Ac3H11_44 0.25 +0.6 41,350 + Ac3H11_44 0.25 +0.5 41,350 + Ac3H11_44 0.25 +2.2 41,351 - Ac3H11_44 0.25 -0.9 41,351 - Ac3H11_44 0.25 -0.4 41,471 + Ac3H11_44 0.36 +1.2 41,472 - Ac3H11_44 0.36 +0.9 41,719 + Ac3H11_44 0.59 +1.6 41,720 - Ac3H11_44 0.59 +2.3 41,720 - Ac3H11_44 0.59 +3.1 41,720 - Ac3H11_44 0.59 +0.9 41,720 - Ac3H11_44 0.59 +2.2 41,720 - Ac3H11_44 0.59 +2.1 41,743 + Ac3H11_44 0.61 +1.8 41,743 + Ac3H11_44 0.61 +2.0 41,743 + Ac3H11_44 0.61 +1.7 41,743 + Ac3H11_44 0.61 +0.6 41,743 + Ac3H11_44 0.61 +0.3 41,743 + Ac3H11_44 0.61 +0.6 41,744 - Ac3H11_44 0.61 +2.0 41,744 - Ac3H11_44 0.61 +1.5 41,744 - Ac3H11_44 0.61 +1.0 41,744 - Ac3H11_44 0.61 +1.7 41,744 - Ac3H11_44 0.61 +1.9 41,744 - Ac3H11_44 0.61 +2.6 41,744 - Ac3H11_44 0.61 +2.4 41,744 - Ac3H11_44 0.61 +2.0 41,776 + Ac3H11_44 0.64 +1.0 41,776 + Ac3H11_44 0.64 +2.2 41,776 + Ac3H11_44 0.64 +0.4 41,845 + Ac3H11_44 0.71 +1.9 41,846 - Ac3H11_44 0.71 +2.3 41,866 + Ac3H11_44 0.73 +1.0 41,866 + Ac3H11_44 0.73 +1.4 41,867 - Ac3H11_44 0.73 +1.2 41,867 - Ac3H11_44 0.73 +0.6 41,867 - Ac3H11_44 0.73 +1.8 41,867 - Ac3H11_44 0.73 +2.2 41,867 - Ac3H11_44 0.73 +0.7 41,867 - Ac3H11_44 0.73 +3.0 41,887 + Ac3H11_44 0.75 +2.5 42,068 - +1.9 42,068 - +2.7 42,068 - +2.9 42,203 + Ac3H11_45 0.55 +0.8 42,203 + Ac3H11_45 0.55 +1.1 42,203 + Ac3H11_45 0.55 +1.3 42,203 + Ac3H11_45 0.55 +1.0 42,203 + Ac3H11_45 0.55 +1.0 42,203 + Ac3H11_45 0.55 +0.6 42,203 + Ac3H11_45 0.55 +1.5 42,203 + Ac3H11_45 0.55 +2.2 42,203 + Ac3H11_45 0.55 +1.1 42,203 + Ac3H11_45 0.55 +1.1 42,203 + Ac3H11_45 0.55 +0.7 42,203 + Ac3H11_45 0.55 +2.2 42,203 + Ac3H11_45 0.55 +1.8 42,203 + Ac3H11_45 0.55 +0.8 42,204 - Ac3H11_45 0.56 +1.9 42,204 - Ac3H11_45 0.56 +0.7 42,204 - Ac3H11_45 0.56 +1.9 42,204 - Ac3H11_45 0.56 +2.7 42,204 - Ac3H11_45 0.56 +1.4 42,204 - Ac3H11_45 0.56 +0.6 42,204 - Ac3H11_45 0.56 +1.0 42,204 - Ac3H11_45 0.56 +0.5 42,204 - Ac3H11_45 0.56 +0.2 42,204 - Ac3H11_45 0.56 +1.3 42,204 - Ac3H11_45 0.56 +1.4 42,204 - Ac3H11_45 0.56 +1.8 42,204 - Ac3H11_45 0.56 +2.2 42,204 - Ac3H11_45 0.56 +1.5 42,204 - Ac3H11_45 0.56 +2.1 42,205 + Ac3H11_45 0.56 +3.1 42,205 + Ac3H11_45 0.56 -0.2 42,205 + Ac3H11_45 0.56 +2.1 42,205 + Ac3H11_45 0.56 +2.0 42,205 + Ac3H11_45 0.56 +0.6 42,205 + Ac3H11_45 0.56 +1.5 42,205 + Ac3H11_45 0.56 +1.8 42,205 + Ac3H11_45 0.56 +1.8 42,220 + Ac3H11_45 0.67 +1.7 42,220 + Ac3H11_45 0.67 +0.5 42,272 + +1.2 42,272 + +0.6 42,272 + +0.5 42,272 + +1.0 42,272 + -1.2 42,272 + -0.1 42,272 + +0.5 42,272 + +0.2 42,272 + +0.0 42,272 + +0.1 42,329 + +1.0 42,329 + -0.3
Or see this region's nucleotide sequence