Experiment: Nickel (II) chloride 0.6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_1209 and Ac3H11_1210 are separated by 118 nucleotides Ac3H11_1210 and Ac3H11_1211 overlap by 4 nucleotides
Ac3H11_1209 - Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42), at 23,110 to 25,875
_1209
Ac3H11_1210 - transcriptional regulator, TetR family, at 25,994 to 26,725
_1210
Ac3H11_1211 - Outer membrane protein assembly factor YaeT precursor, at 26,722 to 28,170
_1211
Position (kb) 25
26
27 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 25.112 kb on - strand, within Ac3H11_1209 at 25.142 kb on - strand, within Ac3H11_1209 at 25.268 kb on - strand, within Ac3H11_1209 at 25.333 kb on + strand, within Ac3H11_1209 at 25.334 kb on - strand, within Ac3H11_1209 at 25.343 kb on - strand, within Ac3H11_1209 at 25.343 kb on - strand, within Ac3H11_1209 at 25.873 kb on + strand at 25.874 kb on - strand at 25.982 kb on + strand at 25.983 kb on - strand at 26.201 kb on + strand, within Ac3H11_1210 at 26.201 kb on + strand, within Ac3H11_1210 at 26.201 kb on + strand, within Ac3H11_1210 at 26.201 kb on + strand, within Ac3H11_1210 at 26.216 kb on + strand, within Ac3H11_1210 at 26.216 kb on + strand, within Ac3H11_1210 at 26.217 kb on - strand, within Ac3H11_1210 at 26.297 kb on + strand, within Ac3H11_1210 at 26.298 kb on - strand, within Ac3H11_1210 at 26.298 kb on - strand, within Ac3H11_1210 at 26.488 kb on + strand, within Ac3H11_1210 at 26.489 kb on - strand, within Ac3H11_1210 at 27.587 kb on - strand, within Ac3H11_1211 at 27.587 kb on - strand, within Ac3H11_1211
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 0.6 mM remove 25,112 - Ac3H11_1209 0.72 -1.4 25,142 - Ac3H11_1209 0.73 +1.0 25,268 - Ac3H11_1209 0.78 -2.2 25,333 + Ac3H11_1209 0.80 -0.1 25,334 - Ac3H11_1209 0.80 -0.2 25,343 - Ac3H11_1209 0.81 -0.9 25,343 - Ac3H11_1209 0.81 -1.9 25,873 + -0.3 25,874 - -0.1 25,982 + -0.2 25,983 - -0.7 26,201 + Ac3H11_1210 0.28 +0.2 26,201 + Ac3H11_1210 0.28 +0.6 26,201 + Ac3H11_1210 0.28 +0.1 26,201 + Ac3H11_1210 0.28 +0.1 26,216 + Ac3H11_1210 0.30 +0.7 26,216 + Ac3H11_1210 0.30 +0.4 26,217 - Ac3H11_1210 0.30 +0.0 26,297 + Ac3H11_1210 0.41 +0.3 26,298 - Ac3H11_1210 0.42 -0.5 26,298 - Ac3H11_1210 0.42 -0.3 26,488 + Ac3H11_1210 0.67 +0.2 26,489 - Ac3H11_1210 0.68 -0.3 27,587 - Ac3H11_1211 0.60 +0.3 27,587 - Ac3H11_1211 0.60 -3.0
Or see this region's nucleotide sequence