Experiment: Darkness for 72h then Flask growth in 60 uE constant light
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ho1 and Synpcc7942_1859 are separated by 123 nucleotides Synpcc7942_1859 and ycf29 overlap by 12 nucleotides ycf29 and natB are separated by 157 nucleotides
Synpcc7942_1858: ho1 - Heme oxygenase (decyclizing), at 1,926,893 to 1,927,609
ho1
Synpcc7942_1859: Synpcc7942_1859 - diguanylate cyclase/phosphodiesterase with PAS/PAC sensor(s), at 1,927,733 to 1,930,486
_1859
Synpcc7942_1860: ycf29 - two component transcriptional regulator, LuxR family, at 1,930,475 to 1,931,182
ycf29
Synpcc7942_1861: natB - periplasmic binding protein of ABC transporter for natural amino acids, at 1,931,340 to 1,932,605
natB
Position (kb)
1927
1928
1929
1930
1931 Strain fitness (log2 ratio)
-2
-1
0
1 at 1927.679 kb on + strand at 1927.679 kb on + strand at 1927.687 kb on - strand at 1927.687 kb on - strand at 1927.725 kb on + strand at 1927.733 kb on - strand at 1927.740 kb on + strand at 1927.752 kb on + strand at 1927.815 kb on - strand at 1927.815 kb on - strand at 1927.815 kb on - strand at 1927.842 kb on - strand at 1927.855 kb on - strand at 1927.864 kb on + strand at 1927.864 kb on + strand at 1927.881 kb on - strand at 1927.881 kb on - strand at 1927.889 kb on + strand at 1927.907 kb on - strand at 1927.965 kb on - strand at 1927.980 kb on - strand at 1928.022 kb on - strand, within Synpcc7942_1859 at 1928.031 kb on + strand, within Synpcc7942_1859 at 1928.076 kb on - strand, within Synpcc7942_1859 at 1928.076 kb on - strand, within Synpcc7942_1859 at 1928.159 kb on - strand, within Synpcc7942_1859 at 1928.159 kb on - strand, within Synpcc7942_1859 at 1928.225 kb on + strand, within Synpcc7942_1859 at 1928.300 kb on + strand, within Synpcc7942_1859 at 1928.306 kb on - strand, within Synpcc7942_1859 at 1928.307 kb on - strand, within Synpcc7942_1859 at 1928.307 kb on - strand, within Synpcc7942_1859 at 1928.308 kb on - strand, within Synpcc7942_1859 at 1928.408 kb on - strand, within Synpcc7942_1859 at 1928.424 kb on + strand, within Synpcc7942_1859 at 1928.431 kb on + strand, within Synpcc7942_1859 at 1928.573 kb on - strand, within Synpcc7942_1859 at 1928.573 kb on - strand at 1928.573 kb on - strand, within Synpcc7942_1859 at 1928.592 kb on - strand, within Synpcc7942_1859 at 1928.655 kb on - strand, within Synpcc7942_1859 at 1928.677 kb on - strand, within Synpcc7942_1859 at 1928.677 kb on - strand, within Synpcc7942_1859 at 1928.735 kb on - strand, within Synpcc7942_1859 at 1928.774 kb on + strand, within Synpcc7942_1859 at 1928.905 kb on + strand, within Synpcc7942_1859 at 1928.919 kb on - strand, within Synpcc7942_1859 at 1928.999 kb on - strand, within Synpcc7942_1859 at 1929.004 kb on + strand, within Synpcc7942_1859 at 1929.110 kb on - strand, within Synpcc7942_1859 at 1929.254 kb on + strand at 1929.265 kb on + strand, within Synpcc7942_1859 at 1929.348 kb on + strand, within Synpcc7942_1859 at 1929.555 kb on + strand, within Synpcc7942_1859 at 1929.563 kb on - strand, within Synpcc7942_1859 at 1929.599 kb on - strand, within Synpcc7942_1859 at 1929.636 kb on - strand, within Synpcc7942_1859 at 1929.636 kb on - strand, within Synpcc7942_1859 at 1929.700 kb on - strand, within Synpcc7942_1859 at 1929.732 kb on + strand, within Synpcc7942_1859 at 1929.740 kb on - strand, within Synpcc7942_1859 at 1929.740 kb on - strand, within Synpcc7942_1859 at 1929.745 kb on - strand, within Synpcc7942_1859 at 1929.802 kb on + strand, within Synpcc7942_1859 at 1929.812 kb on + strand, within Synpcc7942_1859 at 1929.823 kb on + strand, within Synpcc7942_1859 at 1929.831 kb on - strand, within Synpcc7942_1859 at 1929.859 kb on + strand, within Synpcc7942_1859 at 1929.927 kb on + strand, within Synpcc7942_1859 at 1929.993 kb on - strand, within Synpcc7942_1859 at 1930.016 kb on - strand, within Synpcc7942_1859 at 1930.068 kb on + strand, within Synpcc7942_1859 at 1930.097 kb on - strand, within Synpcc7942_1859 at 1930.166 kb on + strand, within Synpcc7942_1859 at 1930.193 kb on + strand, within Synpcc7942_1859 at 1930.201 kb on - strand, within Synpcc7942_1859 at 1930.257 kb on - strand at 1930.315 kb on + strand at 1930.318 kb on - strand at 1930.353 kb on + strand at 1930.418 kb on - strand at 1930.478 kb on + strand at 1931.194 kb on + strand at 1931.194 kb on + strand at 1931.196 kb on + strand at 1931.200 kb on - strand at 1931.202 kb on - strand at 1931.233 kb on - strand at 1931.241 kb on + strand at 1931.248 kb on - strand at 1931.248 kb on - strand at 1931.248 kb on - strand at 1931.248 kb on - strand at 1931.260 kb on - strand at 1931.279 kb on + strand at 1931.281 kb on - strand at 1931.287 kb on - strand at 1931.293 kb on - strand at 1931.298 kb on + strand at 1931.306 kb on - strand at 1931.320 kb on + strand at 1931.350 kb on - strand at 1931.350 kb on - strand at 1931.364 kb on - strand at 1931.364 kb on - strand at 1931.398 kb on + strand at 1931.407 kb on - strand at 1931.417 kb on - strand at 1931.464 kb on - strand at 1931.464 kb on - strand at 1931.484 kb on + strand, within natB
Per-strain Table
Position Strand Gene LocusTag Fraction Darkness for 72h then Flask growth in 60 uE constant light remove 1,927,679 + +0.5 1,927,679 + -1.1 1,927,687 - +0.7 1,927,687 - +0.5 1,927,725 + +0.3 1,927,733 - -0.4 1,927,740 + -0.7 1,927,752 + -1.0 1,927,815 - -0.4 1,927,815 - +1.0 1,927,815 - +0.6 1,927,842 - +0.4 1,927,855 - -0.0 1,927,864 + -0.1 1,927,864 + -0.2 1,927,881 - +0.7 1,927,881 - -0.7 1,927,889 + +0.4 1,927,907 - +0.3 1,927,965 - -0.0 1,927,980 - +0.1 1,928,022 - Synpcc7942_1859 0.10 -0.0 1,928,031 + Synpcc7942_1859 0.11 -0.4 1,928,076 - Synpcc7942_1859 0.12 +0.0 1,928,076 - Synpcc7942_1859 0.12 +0.0 1,928,159 - Synpcc7942_1859 0.15 +0.2 1,928,159 - Synpcc7942_1859 0.15 -0.3 1,928,225 + Synpcc7942_1859 0.18 +0.1 1,928,300 + Synpcc7942_1859 0.21 +0.5 1,928,306 - Synpcc7942_1859 0.21 -0.7 1,928,307 - Synpcc7942_1859 0.21 -0.3 1,928,307 - Synpcc7942_1859 0.21 -0.3 1,928,308 - Synpcc7942_1859 0.21 -0.7 1,928,408 - Synpcc7942_1859 0.25 -0.3 1,928,424 + Synpcc7942_1859 0.25 -1.4 1,928,431 + Synpcc7942_1859 0.25 -0.2 1,928,573 - Synpcc7942_1859 0.31 -0.4 1,928,573 - -0.4 1,928,573 - Synpcc7942_1859 0.31 +0.5 1,928,592 - Synpcc7942_1859 0.31 -0.2 1,928,655 - Synpcc7942_1859 0.33 -0.3 1,928,677 - Synpcc7942_1859 0.34 -0.4 1,928,677 - Synpcc7942_1859 0.34 +0.1 1,928,735 - Synpcc7942_1859 0.36 +0.1 1,928,774 + Synpcc7942_1859 0.38 -0.0 1,928,905 + Synpcc7942_1859 0.43 -0.9 1,928,919 - Synpcc7942_1859 0.43 -0.9 1,928,999 - Synpcc7942_1859 0.46 -0.2 1,929,004 + Synpcc7942_1859 0.46 +0.1 1,929,110 - Synpcc7942_1859 0.50 -0.2 1,929,254 + +1.2 1,929,265 + Synpcc7942_1859 0.56 +0.3 1,929,348 + Synpcc7942_1859 0.59 +0.1 1,929,555 + Synpcc7942_1859 0.66 -0.6 1,929,563 - Synpcc7942_1859 0.66 -0.9 1,929,599 - Synpcc7942_1859 0.68 -0.1 1,929,636 - Synpcc7942_1859 0.69 +0.2 1,929,636 - Synpcc7942_1859 0.69 -0.1 1,929,700 - Synpcc7942_1859 0.71 +0.3 1,929,732 + Synpcc7942_1859 0.73 -0.2 1,929,740 - Synpcc7942_1859 0.73 -0.9 1,929,740 - Synpcc7942_1859 0.73 +0.1 1,929,745 - Synpcc7942_1859 0.73 +1.1 1,929,802 + Synpcc7942_1859 0.75 -0.4 1,929,812 + Synpcc7942_1859 0.75 -0.2 1,929,823 + Synpcc7942_1859 0.76 +0.6 1,929,831 - Synpcc7942_1859 0.76 -0.0 1,929,859 + Synpcc7942_1859 0.77 -0.9 1,929,927 + Synpcc7942_1859 0.80 +0.1 1,929,993 - Synpcc7942_1859 0.82 +0.0 1,930,016 - Synpcc7942_1859 0.83 +0.0 1,930,068 + Synpcc7942_1859 0.85 +0.2 1,930,097 - Synpcc7942_1859 0.86 -0.0 1,930,166 + Synpcc7942_1859 0.88 +0.1 1,930,193 + Synpcc7942_1859 0.89 +0.0 1,930,201 - Synpcc7942_1859 0.90 -0.5 1,930,257 - -0.1 1,930,315 + -0.1 1,930,318 - -0.8 1,930,353 + -0.5 1,930,418 - -1.2 1,930,478 + -0.4 1,931,194 + +0.5 1,931,194 + +0.5 1,931,196 + +0.3 1,931,200 - +0.1 1,931,202 - -0.1 1,931,233 - -2.1 1,931,241 + -0.4 1,931,248 - +1.0 1,931,248 - +0.2 1,931,248 - -0.6 1,931,248 - -0.4 1,931,260 - +0.6 1,931,279 + +0.1 1,931,281 - -0.6 1,931,287 - -0.6 1,931,293 - -1.8 1,931,298 + +0.2 1,931,306 - -0.1 1,931,320 + +0.1 1,931,350 - +0.5 1,931,350 - -0.3 1,931,364 - -0.0 1,931,364 - +0.4 1,931,398 + +0.0 1,931,407 - +0.3 1,931,417 - +0.0 1,931,464 - +0.1 1,931,464 - +0.1 1,931,484 + natB Synpcc7942_1861 0.11 +0.6
Or see this region's nucleotide sequence