Experiment: Darkness for 72h then Flask growth in 60 uE constant light
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_0646 and Synpcc7942_0647 are separated by 203 nucleotides Synpcc7942_0647 and Synpcc7942_0648 are separated by 104 nucleotides Synpcc7942_0648 and rpoD1 are separated by 72 nucleotides
Synpcc7942_0646: Synpcc7942_0646 - hypothetical protein, at 640,765 to 640,992
_0646
Synpcc7942_0647: Synpcc7942_0647 - hypothetical protein, at 641,196 to 641,738
_0647
Synpcc7942_0648: Synpcc7942_0648 - hypothetical protein, at 641,843 to 642,694
_0648
Synpcc7942_0649: rpoD1 - RNA polymerase sigma factor RpoD, at 642,767 to 643,966
rpoD1
Position (kb)
641
642
643 Strain fitness (log2 ratio)
-1
0
1 at 640.905 kb on - strand, within Synpcc7942_0646 at 640.931 kb on - strand, within Synpcc7942_0646 at 640.956 kb on + strand, within Synpcc7942_0646 at 640.986 kb on + strand at 641.015 kb on + strand at 641.025 kb on + strand at 641.028 kb on - strand at 641.051 kb on + strand at 641.053 kb on + strand at 641.059 kb on - strand at 641.059 kb on - strand at 641.069 kb on + strand at 641.086 kb on + strand at 641.120 kb on + strand at 641.120 kb on + strand at 641.123 kb on - strand at 641.125 kb on + strand at 641.166 kb on + strand at 641.335 kb on + strand, within Synpcc7942_0647 at 641.343 kb on - strand, within Synpcc7942_0647 at 641.371 kb on - strand, within Synpcc7942_0647 at 641.391 kb on - strand, within Synpcc7942_0647 at 641.425 kb on + strand, within Synpcc7942_0647 at 641.455 kb on - strand, within Synpcc7942_0647 at 641.535 kb on - strand, within Synpcc7942_0647 at 641.549 kb on - strand, within Synpcc7942_0647 at 641.553 kb on + strand, within Synpcc7942_0647 at 641.554 kb on - strand, within Synpcc7942_0647 at 641.649 kb on + strand, within Synpcc7942_0647 at 641.699 kb on - strand at 641.699 kb on - strand at 641.728 kb on - strand at 641.752 kb on + strand at 641.774 kb on + strand at 641.774 kb on + strand at 641.774 kb on + strand at 641.774 kb on + strand at 641.782 kb on - strand at 641.782 kb on - strand at 641.807 kb on - strand at 642.119 kb on - strand, within Synpcc7942_0648 at 642.248 kb on + strand, within Synpcc7942_0648 at 642.350 kb on + strand, within Synpcc7942_0648 at 642.584 kb on - strand, within Synpcc7942_0648 at 642.758 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Darkness for 72h then Flask growth in 60 uE constant light remove 640,905 - Synpcc7942_0646 0.61 -0.1 640,931 - Synpcc7942_0646 0.73 -1.3 640,956 + Synpcc7942_0646 0.84 -0.0 640,986 + -0.1 641,015 + -0.5 641,025 + +0.5 641,028 - -0.0 641,051 + -0.4 641,053 + -0.1 641,059 - -0.1 641,059 - +0.3 641,069 + -0.0 641,086 + -0.2 641,120 + -0.2 641,120 + +0.2 641,123 - -0.1 641,125 + -0.6 641,166 + +0.3 641,335 + Synpcc7942_0647 0.26 +0.0 641,343 - Synpcc7942_0647 0.27 -0.2 641,371 - Synpcc7942_0647 0.32 +0.1 641,391 - Synpcc7942_0647 0.36 +0.1 641,425 + Synpcc7942_0647 0.42 +0.1 641,455 - Synpcc7942_0647 0.48 +0.6 641,535 - Synpcc7942_0647 0.62 -0.7 641,549 - Synpcc7942_0647 0.65 -0.1 641,553 + Synpcc7942_0647 0.66 -0.0 641,554 - Synpcc7942_0647 0.66 -0.1 641,649 + Synpcc7942_0647 0.83 -0.3 641,699 - +0.4 641,699 - +0.4 641,728 - -0.1 641,752 + +0.6 641,774 + -0.6 641,774 + +0.3 641,774 + -0.5 641,774 + +0.2 641,782 - +0.5 641,782 - -0.1 641,807 - +0.1 642,119 - Synpcc7942_0648 0.32 -1.4 642,248 + Synpcc7942_0648 0.48 -0.4 642,350 + Synpcc7942_0648 0.60 -0.8 642,584 - Synpcc7942_0648 0.87 -0.2 642,758 + -0.1
Or see this region's nucleotide sequence