Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_642

Experiment: L-Malic (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_640 and Pf6N2E2_641 overlap by 34 nucleotidesPf6N2E2_641 and Pf6N2E2_642 are separated by 31 nucleotidesPf6N2E2_642 and Pf6N2E2_643 are separated by 167 nucleotidesPf6N2E2_643 and Pf6N2E2_644 overlap by 4 nucleotides Pf6N2E2_640 - Outer membrane TonB-dependent transducer VreA of trans-envelope signaling system, at 731,648 to 732,280 _640 Pf6N2E2_641 - Extracytoplasmic function (ECF) sigma factor VreI, at 732,247 to 732,822 _641 Pf6N2E2_642 - Sigma factor regulator VreR (cytoplasmic membrane-localized) of trans-envelope signaling system, at 732,854 to 733,801 _642 Pf6N2E2_643 - General secretion pathway protein J, at 733,969 to 734,580 _643 Pf6N2E2_644 - General secretion pathway protein H, at 734,577 to 735,029 _644 Position (kb) 732 733 734Strain fitness (log2 ratio) -1 0 1 2at 731.882 kb on + strand, within Pf6N2E2_640at 731.975 kb on + strand, within Pf6N2E2_640at 731.975 kb on + strand, within Pf6N2E2_640at 731.976 kb on - strand, within Pf6N2E2_640at 732.228 kb on + strandat 732.229 kb on - strandat 732.253 kb on - strandat 732.325 kb on + strand, within Pf6N2E2_641at 732.326 kb on - strand, within Pf6N2E2_641at 732.326 kb on - strand, within Pf6N2E2_641at 732.401 kb on - strand, within Pf6N2E2_641at 732.401 kb on - strand, within Pf6N2E2_641at 732.454 kb on + strand, within Pf6N2E2_641at 732.454 kb on + strand, within Pf6N2E2_641at 732.454 kb on + strand, within Pf6N2E2_641at 732.454 kb on + strand, within Pf6N2E2_641at 732.454 kb on + strand, within Pf6N2E2_641at 732.455 kb on - strand, within Pf6N2E2_641at 732.477 kb on + strand, within Pf6N2E2_641at 732.622 kb on + strand, within Pf6N2E2_641at 732.623 kb on - strand, within Pf6N2E2_641at 732.709 kb on - strand, within Pf6N2E2_641at 733.517 kb on + strand, within Pf6N2E2_642at 733.517 kb on + strand, within Pf6N2E2_642at 733.518 kb on - strand, within Pf6N2E2_642at 733.518 kb on - strand, within Pf6N2E2_642at 733.927 kb on + strandat 733.970 kb on + strandat 733.997 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Malic (C)
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731,882 + Pf6N2E2_640 0.37 -0.9
731,975 + Pf6N2E2_640 0.52 -0.6
731,975 + Pf6N2E2_640 0.52 -1.4
731,976 - Pf6N2E2_640 0.52 -0.3
732,228 + -1.0
732,229 - -0.0
732,253 - -0.2
732,325 + Pf6N2E2_641 0.14 -0.1
732,326 - Pf6N2E2_641 0.14 -1.0
732,326 - Pf6N2E2_641 0.14 -0.5
732,401 - Pf6N2E2_641 0.27 +1.9
732,401 - Pf6N2E2_641 0.27 +1.8
732,454 + Pf6N2E2_641 0.36 -0.5
732,454 + Pf6N2E2_641 0.36 +1.4
732,454 + Pf6N2E2_641 0.36 -0.0
732,454 + Pf6N2E2_641 0.36 +0.4
732,454 + Pf6N2E2_641 0.36 +0.2
732,455 - Pf6N2E2_641 0.36 +0.1
732,477 + Pf6N2E2_641 0.40 +0.1
732,622 + Pf6N2E2_641 0.65 -1.3
732,623 - Pf6N2E2_641 0.65 +0.8
732,709 - Pf6N2E2_641 0.80 -0.3
733,517 + Pf6N2E2_642 0.70 -0.1
733,517 + Pf6N2E2_642 0.70 -0.3
733,518 - Pf6N2E2_642 0.70 -0.1
733,518 - Pf6N2E2_642 0.70 -1.1
733,927 + -0.5
733,970 + +0.6
733,997 + +0.2

Or see this region's nucleotide sequence