Experiment: L-Malic (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_638 and Pf6N2E2_639 are separated by 180 nucleotides Pf6N2E2_639 and Pf6N2E2_640 are separated by 205 nucleotides Pf6N2E2_640 and Pf6N2E2_641 overlap by 34 nucleotides Pf6N2E2_641 and Pf6N2E2_642 are separated by 31 nucleotides
Pf6N2E2_638 - 4-carboxymuconolactone decarboxylase domain/alkylhydroperoxidase AhpD family core domain protein, at 730,480 to 730,920
_638
Pf6N2E2_639 - FIG01285020: hypothetical protein, at 731,101 to 731,442
_639
Pf6N2E2_640 - Outer membrane TonB-dependent transducer VreA of trans-envelope signaling system, at 731,648 to 732,280
_640
Pf6N2E2_641 - Extracytoplasmic function (ECF) sigma factor VreI, at 732,247 to 732,822
_641
Pf6N2E2_642 - Sigma factor regulator VreR (cytoplasmic membrane-localized) of trans-envelope signaling system, at 732,854 to 733,801
_642
Position (kb) 731
732
733 Strain fitness (log2 ratio) -2
-1
0
1
2 at 730.801 kb on + strand, within Pf6N2E2_638 at 730.801 kb on + strand, within Pf6N2E2_638 at 730.802 kb on - strand, within Pf6N2E2_638 at 730.802 kb on - strand, within Pf6N2E2_638 at 731.029 kb on - strand at 731.103 kb on - strand at 731.169 kb on + strand, within Pf6N2E2_639 at 731.170 kb on - strand, within Pf6N2E2_639 at 731.170 kb on - strand, within Pf6N2E2_639 at 731.170 kb on - strand, within Pf6N2E2_639 at 731.170 kb on - strand, within Pf6N2E2_639 at 731.495 kb on + strand at 731.528 kb on + strand at 731.528 kb on + strand at 731.529 kb on - strand at 731.540 kb on - strand at 731.540 kb on - strand at 731.572 kb on + strand at 731.573 kb on - strand at 731.573 kb on - strand at 731.803 kb on + strand, within Pf6N2E2_640 at 731.803 kb on + strand, within Pf6N2E2_640 at 731.804 kb on - strand, within Pf6N2E2_640 at 731.882 kb on + strand, within Pf6N2E2_640 at 731.975 kb on + strand, within Pf6N2E2_640 at 731.975 kb on + strand, within Pf6N2E2_640 at 731.976 kb on - strand, within Pf6N2E2_640 at 732.228 kb on + strand at 732.229 kb on - strand at 732.253 kb on - strand at 732.325 kb on + strand, within Pf6N2E2_641 at 732.326 kb on - strand, within Pf6N2E2_641 at 732.326 kb on - strand, within Pf6N2E2_641 at 732.401 kb on - strand, within Pf6N2E2_641 at 732.401 kb on - strand, within Pf6N2E2_641 at 732.454 kb on + strand, within Pf6N2E2_641 at 732.454 kb on + strand, within Pf6N2E2_641 at 732.454 kb on + strand, within Pf6N2E2_641 at 732.454 kb on + strand, within Pf6N2E2_641 at 732.454 kb on + strand, within Pf6N2E2_641 at 732.455 kb on - strand, within Pf6N2E2_641 at 732.477 kb on + strand, within Pf6N2E2_641 at 732.622 kb on + strand, within Pf6N2E2_641 at 732.623 kb on - strand, within Pf6N2E2_641 at 732.709 kb on - strand, within Pf6N2E2_641
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic (C) remove 730,801 + Pf6N2E2_638 0.73 +1.0 730,801 + Pf6N2E2_638 0.73 -1.8 730,802 - Pf6N2E2_638 0.73 -0.1 730,802 - Pf6N2E2_638 0.73 +0.1 731,029 - +1.0 731,103 - +0.1 731,169 + Pf6N2E2_639 0.20 +0.5 731,170 - Pf6N2E2_639 0.20 -0.4 731,170 - Pf6N2E2_639 0.20 +2.0 731,170 - Pf6N2E2_639 0.20 -0.2 731,170 - Pf6N2E2_639 0.20 -0.1 731,495 + +0.0 731,528 + -0.5 731,528 + +0.3 731,529 - +0.4 731,540 - +1.2 731,540 - -0.4 731,572 + -0.3 731,573 - +0.6 731,573 - -0.9 731,803 + Pf6N2E2_640 0.24 +1.2 731,803 + Pf6N2E2_640 0.24 -2.0 731,804 - Pf6N2E2_640 0.25 -0.1 731,882 + Pf6N2E2_640 0.37 -0.9 731,975 + Pf6N2E2_640 0.52 -0.6 731,975 + Pf6N2E2_640 0.52 -1.4 731,976 - Pf6N2E2_640 0.52 -0.3 732,228 + -1.0 732,229 - -0.0 732,253 - -0.2 732,325 + Pf6N2E2_641 0.14 -0.1 732,326 - Pf6N2E2_641 0.14 -1.0 732,326 - Pf6N2E2_641 0.14 -0.5 732,401 - Pf6N2E2_641 0.27 +1.9 732,401 - Pf6N2E2_641 0.27 +1.8 732,454 + Pf6N2E2_641 0.36 -0.5 732,454 + Pf6N2E2_641 0.36 +1.4 732,454 + Pf6N2E2_641 0.36 -0.0 732,454 + Pf6N2E2_641 0.36 +0.4 732,454 + Pf6N2E2_641 0.36 +0.2 732,455 - Pf6N2E2_641 0.36 +0.1 732,477 + Pf6N2E2_641 0.40 +0.1 732,622 + Pf6N2E2_641 0.65 -1.3 732,623 - Pf6N2E2_641 0.65 +0.8 732,709 - Pf6N2E2_641 0.80 -0.3
Or see this region's nucleotide sequence