Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3535

Experiment: L-Malic (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_3534 and Pf6N2E2_3535 are separated by 390 nucleotidesPf6N2E2_3535 and Pf6N2E2_3536 overlap by 8 nucleotides Pf6N2E2_3534 - Cobalamin biosynthesis protein CobG, at 4,124,063 to 4,125,331 _3534 Pf6N2E2_3535 - Cobalt-precorrin-6y C5-methyltransferase (EC 2.1.1.-) / Cobalt-precorrin-6y C15-methyltransferase [decarboxylating] (EC 2.1.1.-), at 4,125,722 to 4,126,933 _3535 Pf6N2E2_3536 - Cobalt-precorrin-6 synthase, anaerobic, at 4,126,926 to 4,128,023 _3536 Position (kb) 4125 4126 4127Strain fitness (log2 ratio) -2 -1 0 1at 4125.035 kb on - strand, within Pf6N2E2_3534at 4125.353 kb on + strandat 4125.353 kb on + strandat 4125.354 kb on - strandat 4125.354 kb on - strandat 4125.395 kb on - strandat 4125.430 kb on + strandat 4125.430 kb on + strandat 4125.431 kb on - strandat 4125.506 kb on + strandat 4125.506 kb on + strandat 4125.506 kb on + strandat 4125.506 kb on + strandat 4125.506 kb on + strandat 4125.506 kb on + strandat 4125.507 kb on - strandat 4125.507 kb on - strandat 4125.638 kb on + strandat 4125.639 kb on - strandat 4125.639 kb on - strandat 4125.639 kb on - strandat 4125.682 kb on - strandat 4125.741 kb on + strandat 4125.962 kb on + strand, within Pf6N2E2_3535at 4125.962 kb on + strand, within Pf6N2E2_3535at 4125.962 kb on + strand, within Pf6N2E2_3535at 4125.963 kb on - strand, within Pf6N2E2_3535at 4125.963 kb on - strand, within Pf6N2E2_3535at 4125.963 kb on - strand, within Pf6N2E2_3535at 4125.963 kb on - strand, within Pf6N2E2_3535at 4125.963 kb on - strand, within Pf6N2E2_3535at 4126.128 kb on + strand, within Pf6N2E2_3535at 4126.128 kb on + strand, within Pf6N2E2_3535at 4126.128 kb on + strand, within Pf6N2E2_3535at 4126.356 kb on + strand, within Pf6N2E2_3535at 4126.534 kb on + strand, within Pf6N2E2_3535at 4126.534 kb on + strand, within Pf6N2E2_3535at 4126.534 kb on + strand, within Pf6N2E2_3535at 4126.535 kb on - strand, within Pf6N2E2_3535at 4126.535 kb on - strand, within Pf6N2E2_3535at 4126.700 kb on - strand, within Pf6N2E2_3535at 4127.060 kb on - strand, within Pf6N2E2_3536at 4127.060 kb on - strand, within Pf6N2E2_3536at 4127.218 kb on + strand, within Pf6N2E2_3536at 4127.218 kb on + strand, within Pf6N2E2_3536at 4127.218 kb on + strand, within Pf6N2E2_3536at 4127.219 kb on - strand, within Pf6N2E2_3536at 4127.356 kb on - strand, within Pf6N2E2_3536at 4127.499 kb on + strand, within Pf6N2E2_3536at 4127.499 kb on + strand, within Pf6N2E2_3536at 4127.499 kb on + strand, within Pf6N2E2_3536at 4127.500 kb on - strand, within Pf6N2E2_3536at 4127.500 kb on - strand, within Pf6N2E2_3536at 4127.500 kb on - strand, within Pf6N2E2_3536at 4127.500 kb on - strand, within Pf6N2E2_3536at 4127.604 kb on + strand, within Pf6N2E2_3536at 4127.605 kb on - strand, within Pf6N2E2_3536at 4127.605 kb on - strand, within Pf6N2E2_3536

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Malic (C)
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4,125,035 - Pf6N2E2_3534 0.77 -0.1
4,125,353 + -0.0
4,125,353 + +0.8
4,125,354 - -0.8
4,125,354 - -0.6
4,125,395 - +0.8
4,125,430 + -2.3
4,125,430 + -2.6
4,125,431 - +0.2
4,125,506 + -0.6
4,125,506 + -0.1
4,125,506 + +0.0
4,125,506 + +0.5
4,125,506 + +1.3
4,125,506 + +0.8
4,125,507 - +0.2
4,125,507 - +0.4
4,125,638 + -0.1
4,125,639 - +0.8
4,125,639 - +0.6
4,125,639 - -0.1
4,125,682 - +0.8
4,125,741 + -0.6
4,125,962 + Pf6N2E2_3535 0.20 -1.3
4,125,962 + Pf6N2E2_3535 0.20 -0.1
4,125,962 + Pf6N2E2_3535 0.20 +1.3
4,125,963 - Pf6N2E2_3535 0.20 +0.5
4,125,963 - Pf6N2E2_3535 0.20 -0.2
4,125,963 - Pf6N2E2_3535 0.20 -1.6
4,125,963 - Pf6N2E2_3535 0.20 -0.2
4,125,963 - Pf6N2E2_3535 0.20 -0.3
4,126,128 + Pf6N2E2_3535 0.33 +0.1
4,126,128 + Pf6N2E2_3535 0.33 -1.0
4,126,128 + Pf6N2E2_3535 0.33 -0.4
4,126,356 + Pf6N2E2_3535 0.52 +0.0
4,126,534 + Pf6N2E2_3535 0.67 -0.1
4,126,534 + Pf6N2E2_3535 0.67 -2.1
4,126,534 + Pf6N2E2_3535 0.67 -1.6
4,126,535 - Pf6N2E2_3535 0.67 +0.1
4,126,535 - Pf6N2E2_3535 0.67 -0.3
4,126,700 - Pf6N2E2_3535 0.81 -2.0
4,127,060 - Pf6N2E2_3536 0.12 -0.4
4,127,060 - Pf6N2E2_3536 0.12 -0.7
4,127,218 + Pf6N2E2_3536 0.27 +0.1
4,127,218 + Pf6N2E2_3536 0.27 +0.2
4,127,218 + Pf6N2E2_3536 0.27 +0.3
4,127,219 - Pf6N2E2_3536 0.27 -0.3
4,127,356 - Pf6N2E2_3536 0.39 -0.2
4,127,499 + Pf6N2E2_3536 0.52 -0.5
4,127,499 + Pf6N2E2_3536 0.52 -0.6
4,127,499 + Pf6N2E2_3536 0.52 +0.3
4,127,500 - Pf6N2E2_3536 0.52 +0.0
4,127,500 - Pf6N2E2_3536 0.52 +0.4
4,127,500 - Pf6N2E2_3536 0.52 -1.9
4,127,500 - Pf6N2E2_3536 0.52 -0.9
4,127,604 + Pf6N2E2_3536 0.62 -0.1
4,127,605 - Pf6N2E2_3536 0.62 -1.5
4,127,605 - Pf6N2E2_3536 0.62 -0.8

Or see this region's nucleotide sequence