Experiment: Collected from freezer stock
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08465 and MMP_RS08470 are separated by 4 nucleotides MMP_RS08470 and MMP_RS08475 overlap by 4 nucleotides MMP_RS08475 and MMP_RS08480 overlap by 10 nucleotides
MMP_RS08465: MMP_RS08465 - NAD(P)-binding protein, at 1,588,782 to 1,589,972
_RS08465
MMP_RS08470: MMP_RS08470 - ATP-binding cassette domain-containing protein, at 1,589,977 to 1,591,038
_RS08470
MMP_RS08475: MMP_RS08475 - ABC transporter permease, at 1,591,035 to 1,591,817
_RS08475
MMP_RS08480: MMP_RS08480 - molybdate ABC transporter substrate-binding protein, at 1,591,808 to 1,592,611
_RS08480
Position (kb)
1589
1590
1591
1592 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1589.275 kb on - strand at 1589.453 kb on - strand at 1589.456 kb on + strand at 1589.461 kb on - strand at 1589.726 kb on + strand at 1590.013 kb on - strand at 1590.020 kb on + strand at 1590.073 kb on - strand at 1590.202 kb on + strand, within MMP_RS08470 at 1590.226 kb on - strand, within MMP_RS08470 at 1590.454 kb on + strand, within MMP_RS08470 at 1590.592 kb on - strand, within MMP_RS08470 at 1590.593 kb on + strand, within MMP_RS08470 at 1590.692 kb on + strand, within MMP_RS08470 at 1590.812 kb on + strand, within MMP_RS08470 at 1590.841 kb on - strand, within MMP_RS08470 at 1590.842 kb on + strand, within MMP_RS08470 at 1590.842 kb on + strand, within MMP_RS08470 at 1590.862 kb on - strand, within MMP_RS08470 at 1590.947 kb on + strand at 1590.950 kb on - strand at 1590.951 kb on + strand at 1590.970 kb on - strand at 1591.054 kb on - strand at 1591.093 kb on + strand at 1591.150 kb on - strand, within MMP_RS08475 at 1591.177 kb on - strand, within MMP_RS08475 at 1591.178 kb on + strand, within MMP_RS08475 at 1591.183 kb on - strand, within MMP_RS08475 at 1591.286 kb on - strand, within MMP_RS08475 at 1591.374 kb on - strand, within MMP_RS08475 at 1591.374 kb on - strand, within MMP_RS08475 at 1591.374 kb on - strand, within MMP_RS08475 at 1591.374 kb on - strand, within MMP_RS08475 at 1591.374 kb on - strand, within MMP_RS08475 at 1591.375 kb on + strand, within MMP_RS08475 at 1591.515 kb on - strand, within MMP_RS08475 at 1591.728 kb on - strand, within MMP_RS08475 at 1591.729 kb on + strand, within MMP_RS08475 at 1591.729 kb on + strand, within MMP_RS08475 at 1591.733 kb on - strand, within MMP_RS08475 at 1591.755 kb on - strand at 1591.865 kb on - strand at 1591.971 kb on + strand, within MMP_RS08480 at 1592.000 kb on + strand, within MMP_RS08480
Per-strain Table
Position Strand Gene LocusTag Fraction Collected from freezer stock remove 1,589,275 - -0.1 1,589,453 - +0.3 1,589,456 + +0.8 1,589,461 - -1.2 1,589,726 + -1.7 1,590,013 - -0.6 1,590,020 + +1.2 1,590,073 - -0.2 1,590,202 + MMP_RS08470 0.21 -0.1 1,590,226 - MMP_RS08470 0.23 +0.4 1,590,454 + MMP_RS08470 0.45 -0.3 1,590,592 - MMP_RS08470 0.58 -0.4 1,590,593 + MMP_RS08470 0.58 -0.4 1,590,692 + MMP_RS08470 0.67 +0.9 1,590,812 + MMP_RS08470 0.79 -0.1 1,590,841 - MMP_RS08470 0.81 +0.4 1,590,842 + MMP_RS08470 0.81 -0.6 1,590,842 + MMP_RS08470 0.81 -0.6 1,590,862 - MMP_RS08470 0.83 -1.3 1,590,947 + +0.2 1,590,950 - -0.4 1,590,951 + -0.2 1,590,970 - +0.7 1,591,054 - -0.2 1,591,093 + -0.5 1,591,150 - MMP_RS08475 0.15 -0.0 1,591,177 - MMP_RS08475 0.18 +2.7 1,591,178 + MMP_RS08475 0.18 +0.3 1,591,183 - MMP_RS08475 0.19 -0.1 1,591,286 - MMP_RS08475 0.32 +0.0 1,591,374 - MMP_RS08475 0.43 +0.8 1,591,374 - MMP_RS08475 0.43 +0.6 1,591,374 - MMP_RS08475 0.43 -0.7 1,591,374 - MMP_RS08475 0.43 -0.3 1,591,374 - MMP_RS08475 0.43 +0.0 1,591,375 + MMP_RS08475 0.43 -0.4 1,591,515 - MMP_RS08475 0.61 -0.3 1,591,728 - MMP_RS08475 0.89 +0.3 1,591,729 + MMP_RS08475 0.89 -0.6 1,591,729 + MMP_RS08475 0.89 -2.0 1,591,733 - MMP_RS08475 0.89 +0.1 1,591,755 - -0.1 1,591,865 - +1.0 1,591,971 + MMP_RS08480 0.20 -0.5 1,592,000 + MMP_RS08480 0.24 +0.2
Or see this region's nucleotide sequence