Experiment: Collected from freezer stock
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08445 and MMP_RS08450 are separated by 21 nucleotides MMP_RS08450 and MMP_RS08455 are separated by 9 nucleotides MMP_RS08455 and MMP_RS08460 are separated by 305 nucleotides
MMP_RS08445: MMP_RS08445 - DUF2099 family protein, at 1,585,066 to 1,585,917
_RS08445
MMP_RS08450: MMP_RS08450 - isopentenyl phosphate kinase family protein, at 1,585,939 to 1,586,712
_RS08450
MMP_RS08455: MMP_RS08455 - multiprotein bridging factor aMBF1, at 1,586,722 to 1,587,198
_RS08455
MMP_RS08460: MMP_RS08460 - proteasome-activating nucleotidase, at 1,587,504 to 1,588,727
_RS08460
Position (kb)
1586
1587
1588 Strain fitness (log2 ratio)
-1
0
1 at 1586.496 kb on + strand at 1586.508 kb on + strand at 1586.569 kb on + strand at 1586.711 kb on + strand at 1586.711 kb on + strand at 1586.845 kb on - strand, within MMP_RS08455 at 1587.004 kb on + strand, within MMP_RS08455 at 1587.030 kb on - strand, within MMP_RS08455 at 1587.062 kb on + strand, within MMP_RS08455 at 1587.163 kb on + strand at 1587.203 kb on + strand at 1587.253 kb on - strand at 1587.258 kb on + strand at 1587.330 kb on + strand at 1587.330 kb on + strand at 1587.332 kb on + strand at 1587.333 kb on - strand at 1587.334 kb on + strand at 1587.336 kb on - strand at 1587.337 kb on + strand at 1587.351 kb on + strand at 1587.369 kb on + strand at 1587.376 kb on - strand at 1587.377 kb on + strand at 1587.379 kb on + strand at 1587.463 kb on + strand at 1587.465 kb on + strand at 1587.723 kb on + strand, within MMP_RS08460 at 1587.728 kb on + strand, within MMP_RS08460 at 1587.770 kb on - strand, within MMP_RS08460
Per-strain Table
Position Strand Gene LocusTag Fraction Collected from freezer stock remove 1,586,496 + -1.3 1,586,508 + +0.3 1,586,569 + -0.3 1,586,711 + +0.2 1,586,711 + -0.4 1,586,845 - MMP_RS08455 0.26 +0.1 1,587,004 + MMP_RS08455 0.59 -0.7 1,587,030 - MMP_RS08455 0.65 +0.7 1,587,062 + MMP_RS08455 0.71 -0.6 1,587,163 + -0.8 1,587,203 + -0.2 1,587,253 - +0.6 1,587,258 + +0.0 1,587,330 + +0.3 1,587,330 + +0.5 1,587,332 + -0.0 1,587,333 - -1.0 1,587,334 + -0.3 1,587,336 - +0.2 1,587,337 + -0.3 1,587,351 + +0.3 1,587,369 + +0.0 1,587,376 - -0.3 1,587,377 + -0.3 1,587,379 + -0.6 1,587,463 + -0.1 1,587,465 + -0.7 1,587,723 + MMP_RS08460 0.18 +1.1 1,587,728 + MMP_RS08460 0.18 +0.9 1,587,770 - MMP_RS08460 0.22 +0.8
Or see this region's nucleotide sequence