Experiment: Collected from freezer stock
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03345 and MMP_RS03350 are separated by 39 nucleotides MMP_RS03350 and MMP_RS03355 are separated by 108 nucleotides MMP_RS03355 and MMP_RS03360 are separated by 95 nucleotides MMP_RS03360 and MMP_RS03365 are separated by 19 nucleotides MMP_RS03365 and MMP_RS03370 are separated by 39 nucleotides
MMP_RS03345: MMP_RS03345 - hypothetical protein, at 628,612 to 629,844
_RS03345
MMP_RS03350: MMP_RS03350 - winged helix-turn-helix domain-containing protein, at 629,884 to 630,426
_RS03350
MMP_RS03355: MMP_RS03355 - beta-propeller domain-containing protein, at 630,535 to 632,772
_RS03355
MMP_RS03360: MMP_RS03360 - 50S ribosomal protein L24, at 632,868 to 633,056
_RS03360
MMP_RS03365: MMP_RS03365 - 30S ribosomal protein S28e, at 633,076 to 633,306
_RS03365
MMP_RS03370: MMP_RS03370 - 50S ribosomal protein L7Ae, at 633,346 to 633,699
_RS03370
Position (kb) 630
631
632
633 Strain fitness (log2 ratio) -2
-1
0
1 at 629.557 kb on - strand, within MMP_RS03345 at 629.569 kb on + strand, within MMP_RS03345 at 629.608 kb on - strand, within MMP_RS03345 at 629.674 kb on - strand, within MMP_RS03345 at 629.754 kb on - strand at 629.760 kb on - strand at 629.768 kb on - strand at 629.790 kb on + strand at 629.801 kb on - strand at 629.802 kb on + strand at 629.802 kb on + strand at 629.804 kb on + strand at 629.805 kb on - strand at 629.845 kb on - strand at 629.883 kb on - strand at 629.952 kb on + strand, within MMP_RS03350 at 630.202 kb on + strand, within MMP_RS03350 at 630.203 kb on - strand, within MMP_RS03350 at 630.322 kb on + strand, within MMP_RS03350 at 630.407 kb on - strand at 630.412 kb on - strand at 630.413 kb on + strand at 630.483 kb on - strand at 630.575 kb on - strand at 630.599 kb on + strand at 630.599 kb on + strand at 630.628 kb on - strand at 630.628 kb on - strand at 630.631 kb on + strand at 630.713 kb on + strand at 630.730 kb on + strand at 630.794 kb on + strand, within MMP_RS03355 at 631.032 kb on + strand, within MMP_RS03355 at 631.055 kb on + strand, within MMP_RS03355 at 631.341 kb on - strand, within MMP_RS03355 at 631.352 kb on + strand, within MMP_RS03355 at 631.818 kb on + strand, within MMP_RS03355 at 631.818 kb on + strand, within MMP_RS03355 at 631.879 kb on - strand, within MMP_RS03355 at 631.910 kb on + strand, within MMP_RS03355 at 631.917 kb on + strand, within MMP_RS03355 at 632.019 kb on + strand, within MMP_RS03355 at 632.315 kb on + strand, within MMP_RS03355 at 632.347 kb on - strand, within MMP_RS03355 at 632.348 kb on + strand, within MMP_RS03355 at 632.361 kb on + strand, within MMP_RS03355 at 632.510 kb on - strand, within MMP_RS03355 at 632.582 kb on - strand at 632.589 kb on + strand at 632.589 kb on + strand at 632.619 kb on + strand at 632.739 kb on + strand at 632.739 kb on + strand at 632.742 kb on - strand at 632.837 kb on - strand at 633.691 kb on - strand at 633.692 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Collected from freezer stock remove 629,557 - MMP_RS03345 0.77 +0.1 629,569 + MMP_RS03345 0.78 +1.3 629,608 - MMP_RS03345 0.81 -0.2 629,674 - MMP_RS03345 0.86 +0.2 629,754 - -0.3 629,760 - +0.3 629,768 - -0.3 629,790 + -0.5 629,801 - +0.6 629,802 + -0.1 629,802 + -0.2 629,804 + +0.3 629,805 - +0.5 629,845 - +0.2 629,883 - -0.1 629,952 + MMP_RS03350 0.13 +0.1 630,202 + MMP_RS03350 0.59 -0.3 630,203 - MMP_RS03350 0.59 -0.1 630,322 + MMP_RS03350 0.81 -0.1 630,407 - +0.1 630,412 - -0.1 630,413 + -0.1 630,483 - +0.1 630,575 - -0.3 630,599 + -0.2 630,599 + +0.5 630,628 - -0.1 630,628 - -0.0 630,631 + -0.1 630,713 + +0.4 630,730 + -0.0 630,794 + MMP_RS03355 0.12 -0.1 631,032 + MMP_RS03355 0.22 -0.2 631,055 + MMP_RS03355 0.23 -0.6 631,341 - MMP_RS03355 0.36 -0.4 631,352 + MMP_RS03355 0.37 -0.6 631,818 + MMP_RS03355 0.57 +0.3 631,818 + MMP_RS03355 0.57 +0.3 631,879 - MMP_RS03355 0.60 +0.0 631,910 + MMP_RS03355 0.61 +0.7 631,917 + MMP_RS03355 0.62 +0.6 632,019 + MMP_RS03355 0.66 -1.1 632,315 + MMP_RS03355 0.80 -0.0 632,347 - MMP_RS03355 0.81 -0.9 632,348 + MMP_RS03355 0.81 -0.2 632,361 + MMP_RS03355 0.82 -0.6 632,510 - MMP_RS03355 0.88 +0.4 632,582 - -0.1 632,589 + -0.2 632,589 + -0.2 632,619 + -0.4 632,739 + +0.5 632,739 + -0.2 632,742 - -0.1 632,837 - -0.1 633,691 - -1.5 633,692 + -2.4
Or see this region's nucleotide sequence