Experiment: Collected from freezer stock
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02015 and MMP_RS02020 are separated by 5 nucleotides MMP_RS02020 and MMP_RS02025 are separated by 4 nucleotides
MMP_RS02015: MMP_RS02015 - amidohydrolase, at 374,873 to 376,156
_RS02015
MMP_RS02020: MMP_RS02020 - gamma-glutamylcyclotransferase, at 376,162 to 376,485
_RS02020
MMP_RS02025: MMP_RS02025 - DNA-directed DNA polymerase, at 376,490 to 378,844
_RS02025
Position (kb) 376
377 Strain fitness (log2 ratio) -1
0
1 at 375.479 kb on - strand, within MMP_RS02015 at 375.480 kb on + strand, within MMP_RS02015 at 375.518 kb on - strand, within MMP_RS02015 at 375.626 kb on + strand, within MMP_RS02015 at 375.665 kb on - strand, within MMP_RS02015 at 375.667 kb on - strand, within MMP_RS02015 at 375.835 kb on + strand, within MMP_RS02015 at 376.049 kb on + strand at 376.127 kb on + strand at 376.131 kb on - strand at 376.132 kb on + strand at 376.179 kb on + strand at 376.221 kb on - strand, within MMP_RS02020 at 376.300 kb on - strand, within MMP_RS02020 at 376.318 kb on + strand, within MMP_RS02020 at 376.385 kb on - strand, within MMP_RS02020 at 376.479 kb on + strand at 376.568 kb on - strand at 376.679 kb on - strand at 376.682 kb on + strand at 376.682 kb on + strand at 376.691 kb on - strand at 376.691 kb on - strand at 376.691 kb on - strand at 376.730 kb on - strand, within MMP_RS02025 at 376.768 kb on - strand, within MMP_RS02025 at 376.789 kb on - strand, within MMP_RS02025 at 376.974 kb on + strand, within MMP_RS02025 at 377.239 kb on + strand, within MMP_RS02025
Per-strain Table
Position Strand Gene LocusTag Fraction Collected from freezer stock remove 375,479 - MMP_RS02015 0.47 +0.1 375,480 + MMP_RS02015 0.47 +0.8 375,518 - MMP_RS02015 0.50 -0.2 375,626 + MMP_RS02015 0.59 -0.3 375,665 - MMP_RS02015 0.62 +0.4 375,667 - MMP_RS02015 0.62 -0.5 375,835 + MMP_RS02015 0.75 +1.1 376,049 + -0.3 376,127 + -0.3 376,131 - +1.1 376,132 + +0.2 376,179 + -0.4 376,221 - MMP_RS02020 0.18 -0.2 376,300 - MMP_RS02020 0.43 -0.2 376,318 + MMP_RS02020 0.48 -0.6 376,385 - MMP_RS02020 0.69 +0.4 376,479 + -0.5 376,568 - -0.1 376,679 - +0.1 376,682 + +0.2 376,682 + +0.3 376,691 - +0.3 376,691 - +0.2 376,691 - +0.7 376,730 - MMP_RS02025 0.10 +0.3 376,768 - MMP_RS02025 0.12 -0.2 376,789 - MMP_RS02025 0.13 -0.5 376,974 + MMP_RS02025 0.21 -0.1 377,239 + MMP_RS02025 0.32 +0.3
Or see this region's nucleotide sequence