Strain Fitness in Methanococcus maripaludis JJ around MMJJ_RS00175

Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMJJ_RS00165 and MMJJ_RS00170 are separated by 16 nucleotidesMMJJ_RS00170 and MMJJ_RS00175 are separated by 19 nucleotidesMMJJ_RS00175 and MMJJ_RS00180 are separated by 1 nucleotides MMJJ_RS00165: MMJJ_RS00165 - F420-dependent thioredoxin reductase, at 30,618 to 31,520 _RS00165 MMJJ_RS00170: MMJJ_RS00170 - thioredoxin family protein, at 31,537 to 31,884 _RS00170 MMJJ_RS00175: MMJJ_RS00175 - cytochrome c biogenesis CcdA family protein, at 31,904 to 32,557 _RS00175 MMJJ_RS00180: MMJJ_RS00180 - DNA topoisomerase I, at 32,559 to 35,030 _RS00180 Position (kb) 31 32 33Strain fitness (log2 ratio) -2 -1 0 1at 30.912 kb on - strand, within MMJJ_RS00165at 30.913 kb on + strand, within MMJJ_RS00165at 30.932 kb on + strand, within MMJJ_RS00165at 30.947 kb on - strand, within MMJJ_RS00165at 30.947 kb on - strand, within MMJJ_RS00165at 30.948 kb on + strand, within MMJJ_RS00165at 30.948 kb on + strand, within MMJJ_RS00165at 30.951 kb on - strand, within MMJJ_RS00165at 30.967 kb on - strand, within MMJJ_RS00165at 30.968 kb on + strand, within MMJJ_RS00165at 31.002 kb on - strand, within MMJJ_RS00165at 31.003 kb on + strand, within MMJJ_RS00165at 31.003 kb on + strand, within MMJJ_RS00165at 31.064 kb on - strand, within MMJJ_RS00165at 31.086 kb on - strand, within MMJJ_RS00165at 31.086 kb on - strand, within MMJJ_RS00165at 31.086 kb on - strand, within MMJJ_RS00165at 31.086 kb on - strand, within MMJJ_RS00165at 31.088 kb on - strand, within MMJJ_RS00165at 31.088 kb on - strand, within MMJJ_RS00165at 31.088 kb on - strand, within MMJJ_RS00165at 31.089 kb on + strand, within MMJJ_RS00165at 31.093 kb on - strand, within MMJJ_RS00165at 31.094 kb on + strand, within MMJJ_RS00165at 31.100 kb on - strand, within MMJJ_RS00165at 31.114 kb on - strandat 31.120 kb on - strand, within MMJJ_RS00165at 31.125 kb on + strand, within MMJJ_RS00165at 31.142 kb on + strand, within MMJJ_RS00165at 31.180 kb on - strand, within MMJJ_RS00165at 31.198 kb on - strand, within MMJJ_RS00165at 31.198 kb on - strand, within MMJJ_RS00165at 31.253 kb on - strand, within MMJJ_RS00165at 31.284 kb on + strand, within MMJJ_RS00165at 31.300 kb on - strand, within MMJJ_RS00165at 31.304 kb on - strand, within MMJJ_RS00165at 31.306 kb on - strand, within MMJJ_RS00165at 31.404 kb on + strand, within MMJJ_RS00165at 31.481 kb on - strandat 31.481 kb on - strandat 31.482 kb on + strandat 31.562 kb on - strandat 31.562 kb on - strandat 31.566 kb on - strandat 31.566 kb on - strandat 31.567 kb on + strandat 31.567 kb on + strandat 31.568 kb on - strandat 31.569 kb on + strandat 31.569 kb on + strandat 31.569 kb on + strandat 31.569 kb on + strandat 31.593 kb on - strand, within MMJJ_RS00170at 31.594 kb on + strand, within MMJJ_RS00170at 31.594 kb on + strand, within MMJJ_RS00170at 31.616 kb on - strand, within MMJJ_RS00170at 31.616 kb on - strand, within MMJJ_RS00170at 31.620 kb on - strand, within MMJJ_RS00170at 31.621 kb on + strand, within MMJJ_RS00170at 31.722 kb on - strand, within MMJJ_RS00170at 31.722 kb on - strand, within MMJJ_RS00170at 31.722 kb on - strand, within MMJJ_RS00170at 31.723 kb on + strand, within MMJJ_RS00170at 31.723 kb on + strand, within MMJJ_RS00170at 31.723 kb on + strand, within MMJJ_RS00170at 31.725 kb on + strand, within MMJJ_RS00170at 31.734 kb on + strand, within MMJJ_RS00170at 31.768 kb on - strand, within MMJJ_RS00170at 31.773 kb on - strand, within MMJJ_RS00170at 31.850 kb on - strandat 31.853 kb on - strandat 31.890 kb on + strandat 31.962 kb on - strandat 31.971 kb on - strand, within MMJJ_RS00175at 31.972 kb on + strand, within MMJJ_RS00175at 31.972 kb on + strand, within MMJJ_RS00175at 31.984 kb on + strand, within MMJJ_RS00175at 31.988 kb on - strand, within MMJJ_RS00175at 31.988 kb on - strand, within MMJJ_RS00175at 31.989 kb on + strand, within MMJJ_RS00175at 31.990 kb on - strand, within MMJJ_RS00175at 31.991 kb on + strand, within MMJJ_RS00175at 31.991 kb on + strand, within MMJJ_RS00175at 32.005 kb on - strand, within MMJJ_RS00175at 32.045 kb on + strand, within MMJJ_RS00175at 32.129 kb on - strand, within MMJJ_RS00175at 32.248 kb on - strand, within MMJJ_RS00175at 32.249 kb on + strand, within MMJJ_RS00175at 32.256 kb on - strand, within MMJJ_RS00175at 32.272 kb on + strand, within MMJJ_RS00175at 32.314 kb on - strand, within MMJJ_RS00175at 32.314 kb on - strand, within MMJJ_RS00175at 32.315 kb on + strand, within MMJJ_RS00175at 32.316 kb on - strand, within MMJJ_RS00175at 32.316 kb on - strand, within MMJJ_RS00175at 32.317 kb on + strand, within MMJJ_RS00175at 32.375 kb on - strand, within MMJJ_RS00175at 32.402 kb on - strand, within MMJJ_RS00175at 32.430 kb on - strand, within MMJJ_RS00175at 32.431 kb on + strand, within MMJJ_RS00175at 32.466 kb on - strand, within MMJJ_RS00175at 32.482 kb on + strand, within MMJJ_RS00175at 32.495 kb on + strandat 32.502 kb on + strandat 32.502 kb on + strandat 32.503 kb on - strandat 32.503 kb on - strandat 32.504 kb on + strandat 32.504 kb on + strandat 32.504 kb on + strandat 32.504 kb on + strandat 32.515 kb on - strandat 32.516 kb on + strandat 32.518 kb on + strandat 32.545 kb on + strandat 32.545 kb on + strandat 32.560 kb on - strandat 32.595 kb on + strandat 32.636 kb on - strandat 32.636 kb on - strandat 32.729 kb on - strandat 32.748 kb on - strandat 32.766 kb on + strandat 32.819 kb on - strand, within MMJJ_RS00180at 32.820 kb on + strand, within MMJJ_RS00180at 32.822 kb on + strand, within MMJJ_RS00180at 32.896 kb on - strand, within MMJJ_RS00180at 32.897 kb on + strand, within MMJJ_RS00180at 32.897 kb on + strand, within MMJJ_RS00180at 32.897 kb on + strand, within MMJJ_RS00180at 32.900 kb on - strand, within MMJJ_RS00180at 32.901 kb on + strand, within MMJJ_RS00180at 33.041 kb on - strand, within MMJJ_RS00180at 33.074 kb on - strand, within MMJJ_RS00180at 33.123 kb on - strand, within MMJJ_RS00180at 33.159 kb on + strand, within MMJJ_RS00180at 33.175 kb on - strand, within MMJJ_RS00180at 33.213 kb on + strand, within MMJJ_RS00180at 33.243 kb on + strand, within MMJJ_RS00180at 33.256 kb on + strand, within MMJJ_RS00180at 33.262 kb on + strand, within MMJJ_RS00180at 33.270 kb on + strand, within MMJJ_RS00180at 33.270 kb on + strand, within MMJJ_RS00180

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Per-strain Table

Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE
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30,912 - MMJJ_RS00165 0.33 +0.1
30,913 + MMJJ_RS00165 0.33 +0.6
30,932 + MMJJ_RS00165 0.35 -1.8
30,947 - MMJJ_RS00165 0.36 -0.1
30,947 - MMJJ_RS00165 0.36 -0.3
30,948 + MMJJ_RS00165 0.37 +0.0
30,948 + MMJJ_RS00165 0.37 -0.8
30,951 - MMJJ_RS00165 0.37 -0.4
30,967 - MMJJ_RS00165 0.39 +0.2
30,968 + MMJJ_RS00165 0.39 +0.1
31,002 - MMJJ_RS00165 0.43 -0.1
31,003 + MMJJ_RS00165 0.43 -0.4
31,003 + MMJJ_RS00165 0.43 +0.2
31,064 - MMJJ_RS00165 0.49 -1.6
31,086 - MMJJ_RS00165 0.52 +0.3
31,086 - MMJJ_RS00165 0.52 +0.1
31,086 - MMJJ_RS00165 0.52 +0.4
31,086 - MMJJ_RS00165 0.52 -0.5
31,088 - MMJJ_RS00165 0.52 +0.5
31,088 - MMJJ_RS00165 0.52 +0.0
31,088 - MMJJ_RS00165 0.52 +0.1
31,089 + MMJJ_RS00165 0.52 +0.5
31,093 - MMJJ_RS00165 0.53 +0.4
31,094 + MMJJ_RS00165 0.53 +0.4
31,100 - MMJJ_RS00165 0.53 -0.0
31,114 - +0.1
31,120 - MMJJ_RS00165 0.56 -0.7
31,125 + MMJJ_RS00165 0.56 +0.0
31,142 + MMJJ_RS00165 0.58 +0.9
31,180 - MMJJ_RS00165 0.62 +0.2
31,198 - MMJJ_RS00165 0.64 -0.1
31,198 - MMJJ_RS00165 0.64 +0.1
31,253 - MMJJ_RS00165 0.70 -0.1
31,284 + MMJJ_RS00165 0.74 -0.4
31,300 - MMJJ_RS00165 0.76 -0.9
31,304 - MMJJ_RS00165 0.76 -0.3
31,306 - MMJJ_RS00165 0.76 -0.8
31,404 + MMJJ_RS00165 0.87 -0.6
31,481 - -0.3
31,481 - +0.4
31,482 + +0.2
31,562 - +0.4
31,562 - -1.0
31,566 - +0.3
31,566 - +0.0
31,567 + +0.5
31,567 + +0.2
31,568 - -0.1
31,569 + -0.2
31,569 + -0.6
31,569 + -0.2
31,569 + +0.3
31,593 - MMJJ_RS00170 0.16 -1.4
31,594 + MMJJ_RS00170 0.16 -0.1
31,594 + MMJJ_RS00170 0.16 +0.0
31,616 - MMJJ_RS00170 0.23 -0.1
31,616 - MMJJ_RS00170 0.23 -0.5
31,620 - MMJJ_RS00170 0.24 +0.4
31,621 + MMJJ_RS00170 0.24 -0.7
31,722 - MMJJ_RS00170 0.53 +1.7
31,722 - MMJJ_RS00170 0.53 -0.1
31,722 - MMJJ_RS00170 0.53 +0.1
31,723 + MMJJ_RS00170 0.53 +1.0
31,723 + MMJJ_RS00170 0.53 -0.2
31,723 + MMJJ_RS00170 0.53 -0.5
31,725 + MMJJ_RS00170 0.54 -0.2
31,734 + MMJJ_RS00170 0.57 +0.8
31,768 - MMJJ_RS00170 0.66 -0.9
31,773 - MMJJ_RS00170 0.68 +0.5
31,850 - +0.2
31,853 - +0.2
31,890 + +0.2
31,962 - -0.7
31,971 - MMJJ_RS00175 0.10 +0.5
31,972 + MMJJ_RS00175 0.10 +0.4
31,972 + MMJJ_RS00175 0.10 +0.3
31,984 + MMJJ_RS00175 0.12 -0.8
31,988 - MMJJ_RS00175 0.13 -2.4
31,988 - MMJJ_RS00175 0.13 +0.4
31,989 + MMJJ_RS00175 0.13 +0.8
31,990 - MMJJ_RS00175 0.13 +0.5
31,991 + MMJJ_RS00175 0.13 +0.3
31,991 + MMJJ_RS00175 0.13 -0.3
32,005 - MMJJ_RS00175 0.15 -0.2
32,045 + MMJJ_RS00175 0.22 -0.4
32,129 - MMJJ_RS00175 0.34 -0.9
32,248 - MMJJ_RS00175 0.53 -0.0
32,249 + MMJJ_RS00175 0.53 +0.2
32,256 - MMJJ_RS00175 0.54 -0.1
32,272 + MMJJ_RS00175 0.56 -0.6
32,314 - MMJJ_RS00175 0.63 +0.6
32,314 - MMJJ_RS00175 0.63 +0.2
32,315 + MMJJ_RS00175 0.63 +0.6
32,316 - MMJJ_RS00175 0.63 -0.7
32,316 - MMJJ_RS00175 0.63 +0.4
32,317 + MMJJ_RS00175 0.63 +0.4
32,375 - MMJJ_RS00175 0.72 -0.1
32,402 - MMJJ_RS00175 0.76 +0.6
32,430 - MMJJ_RS00175 0.80 -0.0
32,431 + MMJJ_RS00175 0.81 -0.6
32,466 - MMJJ_RS00175 0.86 +0.3
32,482 + MMJJ_RS00175 0.88 +0.4
32,495 + +0.4
32,502 + +0.3
32,502 + -0.2
32,503 - -0.7
32,503 - +0.2
32,504 + -1.0
32,504 + -0.5
32,504 + +0.3
32,504 + +0.1
32,515 - +0.2
32,516 + -1.6
32,518 + -1.1
32,545 + -0.4
32,545 + +0.9
32,560 - +0.4
32,595 + +0.1
32,636 - -0.3
32,636 - -0.4
32,729 - +0.4
32,748 - +0.7
32,766 + -0.4
32,819 - MMJJ_RS00180 0.11 +0.0
32,820 + MMJJ_RS00180 0.11 +0.0
32,822 + MMJJ_RS00180 0.11 +0.5
32,896 - MMJJ_RS00180 0.14 -1.5
32,897 + MMJJ_RS00180 0.14 -0.0
32,897 + MMJJ_RS00180 0.14 -0.7
32,897 + MMJJ_RS00180 0.14 -1.2
32,900 - MMJJ_RS00180 0.14 -1.2
32,901 + MMJJ_RS00180 0.14 -0.5
33,041 - MMJJ_RS00180 0.19 -0.5
33,074 - MMJJ_RS00180 0.21 -0.8
33,123 - MMJJ_RS00180 0.23 -0.6
33,159 + MMJJ_RS00180 0.24 -1.4
33,175 - MMJJ_RS00180 0.25 -0.5
33,213 + MMJJ_RS00180 0.26 -0.0
33,243 + MMJJ_RS00180 0.28 -2.2
33,256 + MMJJ_RS00180 0.28 +0.1
33,262 + MMJJ_RS00180 0.28 -1.6
33,270 + MMJJ_RS00180 0.29 -0.5
33,270 + MMJJ_RS00180 0.29 -0.8

Or see this region's nucleotide sequence