Experiment: CDM1 without Cysteine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS17020 and BBR_RS17025 are separated by 101 nucleotides BBR_RS17025 and BBR_RS17030 are separated by 167 nucleotides BBR_RS17030 and BBR_RS17035 are separated by 23 nucleotides
BBR_RS17020: BBR_RS17020 - type I glutamate--ammonia ligase, at 1,593,859 to 1,595,196
_RS17020
BBR_RS17025: BBR_RS17025 - DUF2252 domain-containing protein, at 1,595,298 to 1,596,734
_RS17025
BBR_RS17030: BBR_RS17030 - bifunctional 1-(5-phosphoribosyl)-5-((5- phosphoribosylamino)methylideneamino)imidazole-4- carboxamide isomerase/phosphoribosylanthranilate isomerase PriA, at 1,596,902 to 1,597,627
_RS17030
BBR_RS17035: BBR_RS17035 - imidazole glycerol phosphate synthase subunit HisH, at 1,597,651 to 1,598,298
_RS17035
Position (kb)
1595
1596
1597 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1595.238 kb on + strand at 1595.311 kb on + strand at 1595.355 kb on + strand at 1595.390 kb on + strand at 1595.450 kb on - strand, within BBR_RS17025 at 1595.450 kb on - strand, within BBR_RS17025 at 1595.544 kb on - strand, within BBR_RS17025 at 1595.563 kb on + strand, within BBR_RS17025 at 1595.571 kb on - strand, within BBR_RS17025 at 1595.624 kb on - strand, within BBR_RS17025 at 1595.626 kb on + strand, within BBR_RS17025 at 1595.629 kb on - strand, within BBR_RS17025 at 1595.684 kb on + strand, within BBR_RS17025 at 1595.839 kb on - strand, within BBR_RS17025 at 1595.892 kb on - strand, within BBR_RS17025 at 1595.947 kb on + strand, within BBR_RS17025 at 1595.947 kb on + strand, within BBR_RS17025 at 1595.947 kb on + strand, within BBR_RS17025 at 1595.947 kb on + strand, within BBR_RS17025 at 1595.947 kb on + strand, within BBR_RS17025 at 1595.947 kb on + strand, within BBR_RS17025 at 1595.955 kb on - strand, within BBR_RS17025 at 1595.955 kb on - strand, within BBR_RS17025 at 1595.955 kb on - strand, within BBR_RS17025 at 1595.955 kb on - strand, within BBR_RS17025 at 1595.955 kb on - strand, within BBR_RS17025 at 1595.955 kb on - strand, within BBR_RS17025 at 1595.955 kb on - strand, within BBR_RS17025 at 1595.955 kb on - strand, within BBR_RS17025 at 1595.955 kb on - strand, within BBR_RS17025 at 1595.967 kb on + strand, within BBR_RS17025 at 1595.975 kb on - strand, within BBR_RS17025 at 1595.981 kb on + strand, within BBR_RS17025 at 1596.012 kb on - strand, within BBR_RS17025 at 1596.014 kb on - strand, within BBR_RS17025 at 1596.114 kb on - strand, within BBR_RS17025 at 1596.213 kb on + strand, within BBR_RS17025 at 1596.213 kb on + strand, within BBR_RS17025 at 1596.234 kb on - strand, within BBR_RS17025 at 1596.308 kb on + strand, within BBR_RS17025 at 1596.337 kb on - strand, within BBR_RS17025 at 1596.339 kb on + strand, within BBR_RS17025 at 1596.339 kb on + strand, within BBR_RS17025 at 1596.352 kb on - strand, within BBR_RS17025 at 1596.378 kb on + strand, within BBR_RS17025 at 1596.379 kb on + strand, within BBR_RS17025 at 1596.471 kb on - strand, within BBR_RS17025 at 1596.530 kb on - strand, within BBR_RS17025 at 1596.540 kb on + strand, within BBR_RS17025 at 1596.575 kb on - strand, within BBR_RS17025 at 1596.575 kb on - strand, within BBR_RS17025 at 1596.576 kb on - strand, within BBR_RS17025 at 1596.643 kb on - strand at 1596.664 kb on - strand at 1596.664 kb on - strand at 1596.688 kb on - strand at 1596.704 kb on + strand at 1596.814 kb on - strand at 1597.109 kb on - strand, within BBR_RS17030 at 1597.146 kb on - strand, within BBR_RS17030 at 1597.173 kb on + strand, within BBR_RS17030 at 1597.191 kb on - strand, within BBR_RS17030 at 1597.227 kb on - strand, within BBR_RS17030 at 1597.252 kb on + strand, within BBR_RS17030 at 1597.258 kb on + strand, within BBR_RS17030 at 1597.276 kb on + strand, within BBR_RS17030 at 1597.288 kb on + strand, within BBR_RS17030 at 1597.292 kb on - strand, within BBR_RS17030 at 1597.298 kb on + strand, within BBR_RS17030 at 1597.413 kb on - strand, within BBR_RS17030 at 1597.430 kb on - strand, within BBR_RS17030 at 1597.442 kb on + strand, within BBR_RS17030 at 1597.471 kb on - strand, within BBR_RS17030 at 1597.484 kb on + strand, within BBR_RS17030 at 1597.500 kb on + strand, within BBR_RS17030 at 1597.543 kb on - strand, within BBR_RS17030 at 1597.597 kb on + strand at 1597.602 kb on + strand at 1597.605 kb on - strand at 1597.656 kb on - strand at 1597.709 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Cysteine remove 1,595,238 + -0.8 1,595,311 + -0.2 1,595,355 + +0.6 1,595,390 + -0.8 1,595,450 - BBR_RS17025 0.11 -3.6 1,595,450 - BBR_RS17025 0.11 -0.7 1,595,544 - BBR_RS17025 0.17 -2.6 1,595,563 + BBR_RS17025 0.18 -1.7 1,595,571 - BBR_RS17025 0.19 -2.2 1,595,624 - BBR_RS17025 0.23 -1.6 1,595,626 + BBR_RS17025 0.23 -1.6 1,595,629 - BBR_RS17025 0.23 -2.8 1,595,684 + BBR_RS17025 0.27 +1.5 1,595,839 - BBR_RS17025 0.38 -1.0 1,595,892 - BBR_RS17025 0.41 -0.6 1,595,947 + BBR_RS17025 0.45 -1.3 1,595,947 + BBR_RS17025 0.45 +0.1 1,595,947 + BBR_RS17025 0.45 -0.1 1,595,947 + BBR_RS17025 0.45 -1.9 1,595,947 + BBR_RS17025 0.45 -0.0 1,595,947 + BBR_RS17025 0.45 -2.9 1,595,955 - BBR_RS17025 0.46 -1.0 1,595,955 - BBR_RS17025 0.46 -0.4 1,595,955 - BBR_RS17025 0.46 -2.6 1,595,955 - BBR_RS17025 0.46 -0.4 1,595,955 - BBR_RS17025 0.46 -1.1 1,595,955 - BBR_RS17025 0.46 -0.5 1,595,955 - BBR_RS17025 0.46 -1.0 1,595,955 - BBR_RS17025 0.46 -0.1 1,595,955 - BBR_RS17025 0.46 -2.1 1,595,967 + BBR_RS17025 0.47 -0.9 1,595,975 - BBR_RS17025 0.47 +1.5 1,595,981 + BBR_RS17025 0.48 -1.0 1,596,012 - BBR_RS17025 0.50 -2.4 1,596,014 - BBR_RS17025 0.50 -0.7 1,596,114 - BBR_RS17025 0.57 -1.0 1,596,213 + BBR_RS17025 0.64 -0.3 1,596,213 + BBR_RS17025 0.64 -0.8 1,596,234 - BBR_RS17025 0.65 -0.3 1,596,308 + BBR_RS17025 0.70 -0.3 1,596,337 - BBR_RS17025 0.72 -1.7 1,596,339 + BBR_RS17025 0.72 +0.6 1,596,339 + BBR_RS17025 0.72 +0.5 1,596,352 - BBR_RS17025 0.73 -0.7 1,596,378 + BBR_RS17025 0.75 -0.6 1,596,379 + BBR_RS17025 0.75 +0.0 1,596,471 - BBR_RS17025 0.82 +0.3 1,596,530 - BBR_RS17025 0.86 +0.2 1,596,540 + BBR_RS17025 0.86 -1.3 1,596,575 - BBR_RS17025 0.89 -1.6 1,596,575 - BBR_RS17025 0.89 -1.5 1,596,576 - BBR_RS17025 0.89 -0.6 1,596,643 - +0.4 1,596,664 - -0.6 1,596,664 - +0.9 1,596,688 - +0.2 1,596,704 + -0.3 1,596,814 - -1.9 1,597,109 - BBR_RS17030 0.29 +0.2 1,597,146 - BBR_RS17030 0.34 -1.4 1,597,173 + BBR_RS17030 0.37 +0.2 1,597,191 - BBR_RS17030 0.40 +0.7 1,597,227 - BBR_RS17030 0.45 -0.0 1,597,252 + BBR_RS17030 0.48 -0.7 1,597,258 + BBR_RS17030 0.49 +0.6 1,597,276 + BBR_RS17030 0.52 +0.1 1,597,288 + BBR_RS17030 0.53 -1.3 1,597,292 - BBR_RS17030 0.54 +0.2 1,597,298 + BBR_RS17030 0.55 -0.3 1,597,413 - BBR_RS17030 0.70 -0.3 1,597,430 - BBR_RS17030 0.73 -3.2 1,597,442 + BBR_RS17030 0.74 -0.1 1,597,471 - BBR_RS17030 0.78 -0.3 1,597,484 + BBR_RS17030 0.80 +0.2 1,597,500 + BBR_RS17030 0.82 -0.1 1,597,543 - BBR_RS17030 0.88 +0.7 1,597,597 + -1.0 1,597,602 + +0.5 1,597,605 - +0.5 1,597,656 - -1.1 1,597,709 + -0.3
Or see this region's nucleotide sequence