Experiment: CDM1 without Cysteine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14330 and BBR_RS14335 are separated by 137 nucleotides BBR_RS14335 and BBR_RS14340 are separated by 148 nucleotides BBR_RS14340 and BBR_RS14345 are separated by 3 nucleotides BBR_RS14345 and BBR_RS14350 are separated by 168 nucleotides BBR_RS14350 and BBR_RS14355 are separated by 225 nucleotides
BBR_RS14330: BBR_RS14330 - glycogen debranching enzyme GlgX, at 990,756 to 992,867
_RS14330
BBR_RS14335: BBR_RS14335 - hypothetical protein, at 993,005 to 993,205
_RS14335
BBR_RS14340: BBR_RS14340 - helix-turn-helix domain-containing protein, at 993,354 to 993,839
_RS14340
BBR_RS14345: BBR_RS14345 - hypothetical protein, at 993,843 to 994,493
_RS14345
BBR_RS14350: BBR_RS14350 - hypothetical protein, at 994,662 to 994,889
_RS14350
BBR_RS14355: BBR_RS14355 - tRNA-Pro, at 995,115 to 995,191
_RS14355
Position (kb) 993
994
995 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 992.910 kb on + strand at 992.917 kb on + strand at 992.917 kb on + strand at 992.956 kb on - strand at 992.962 kb on + strand at 993.000 kb on + strand at 993.101 kb on + strand, within BBR_RS14335 at 993.101 kb on + strand, within BBR_RS14335 at 993.125 kb on - strand, within BBR_RS14335 at 993.132 kb on + strand, within BBR_RS14335 at 993.132 kb on + strand, within BBR_RS14335 at 993.140 kb on - strand, within BBR_RS14335 at 993.181 kb on + strand, within BBR_RS14335 at 993.189 kb on - strand at 993.286 kb on + strand at 993.294 kb on - strand at 993.304 kb on - strand at 993.371 kb on + strand at 993.390 kb on + strand at 993.519 kb on - strand, within BBR_RS14340 at 993.732 kb on + strand, within BBR_RS14340 at 993.742 kb on + strand, within BBR_RS14340 at 993.760 kb on - strand, within BBR_RS14340 at 993.760 kb on - strand, within BBR_RS14340 at 993.809 kb on - strand at 993.874 kb on - strand at 993.943 kb on + strand, within BBR_RS14345 at 993.943 kb on + strand, within BBR_RS14345 at 993.956 kb on + strand, within BBR_RS14345 at 993.985 kb on - strand, within BBR_RS14345 at 993.995 kb on + strand, within BBR_RS14345 at 994.003 kb on - strand, within BBR_RS14345 at 994.003 kb on - strand, within BBR_RS14345 at 994.088 kb on + strand, within BBR_RS14345 at 994.095 kb on + strand, within BBR_RS14345 at 994.095 kb on + strand, within BBR_RS14345 at 994.105 kb on + strand, within BBR_RS14345 at 994.113 kb on - strand, within BBR_RS14345 at 994.219 kb on - strand, within BBR_RS14345 at 994.266 kb on - strand, within BBR_RS14345 at 994.283 kb on + strand, within BBR_RS14345 at 994.298 kb on + strand, within BBR_RS14345 at 994.328 kb on + strand, within BBR_RS14345 at 994.386 kb on - strand, within BBR_RS14345 at 994.428 kb on - strand, within BBR_RS14345 at 994.507 kb on + strand at 994.510 kb on - strand at 994.606 kb on + strand at 994.614 kb on - strand at 994.614 kb on - strand at 994.702 kb on - strand, within BBR_RS14350 at 994.724 kb on - strand, within BBR_RS14350 at 994.729 kb on + strand, within BBR_RS14350 at 994.747 kb on - strand, within BBR_RS14350 at 994.770 kb on + strand, within BBR_RS14350 at 994.794 kb on + strand, within BBR_RS14350 at 994.881 kb on + strand at 994.889 kb on - strand at 995.015 kb on + strand at 995.015 kb on + strand at 995.375 kb on - strand at 995.439 kb on + strand at 995.484 kb on + strand at 995.492 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Cysteine remove 992,910 + -1.7 992,917 + +0.4 992,917 + -0.2 992,956 - +0.7 992,962 + +0.6 993,000 + -0.8 993,101 + BBR_RS14335 0.48 +0.1 993,101 + BBR_RS14335 0.48 -1.7 993,125 - BBR_RS14335 0.60 -0.4 993,132 + BBR_RS14335 0.63 +3.4 993,132 + BBR_RS14335 0.63 +0.1 993,140 - BBR_RS14335 0.67 -0.6 993,181 + BBR_RS14335 0.88 -0.3 993,189 - -0.6 993,286 + +0.3 993,294 - -0.2 993,304 - -0.4 993,371 + +0.4 993,390 + +0.3 993,519 - BBR_RS14340 0.34 -0.7 993,732 + BBR_RS14340 0.78 -0.8 993,742 + BBR_RS14340 0.80 -0.7 993,760 - BBR_RS14340 0.84 -0.2 993,760 - BBR_RS14340 0.84 -0.6 993,809 - +0.4 993,874 - -0.9 993,943 + BBR_RS14345 0.15 +0.6 993,943 + BBR_RS14345 0.15 -0.9 993,956 + BBR_RS14345 0.17 -1.7 993,985 - BBR_RS14345 0.22 -0.8 993,995 + BBR_RS14345 0.23 -0.2 994,003 - BBR_RS14345 0.25 +0.2 994,003 - BBR_RS14345 0.25 -1.5 994,088 + BBR_RS14345 0.38 -0.8 994,095 + BBR_RS14345 0.39 -0.6 994,095 + BBR_RS14345 0.39 -1.2 994,105 + BBR_RS14345 0.40 -0.6 994,113 - BBR_RS14345 0.41 -0.6 994,219 - BBR_RS14345 0.58 +0.2 994,266 - BBR_RS14345 0.65 -1.2 994,283 + BBR_RS14345 0.68 -1.4 994,298 + BBR_RS14345 0.70 -0.3 994,328 + BBR_RS14345 0.75 -1.9 994,386 - BBR_RS14345 0.83 -1.4 994,428 - BBR_RS14345 0.90 -1.2 994,507 + -0.2 994,510 - -0.6 994,606 + +0.1 994,614 - -1.1 994,614 - +0.5 994,702 - BBR_RS14350 0.18 -0.1 994,724 - BBR_RS14350 0.27 -0.1 994,729 + BBR_RS14350 0.29 -0.2 994,747 - BBR_RS14350 0.37 -1.1 994,770 + BBR_RS14350 0.47 -0.1 994,794 + BBR_RS14350 0.58 +0.1 994,881 + -1.2 994,889 - -0.5 995,015 + -1.7 995,015 + -1.5 995,375 - -2.7 995,439 + +0.6 995,484 + -1.7 995,492 + +0.3
Or see this region's nucleotide sequence