Experiment: CDM1 without Cysteine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12220 and BBR_RS12225 are separated by 108 nucleotides BBR_RS12225 and BBR_RS20675 are separated by 278 nucleotides BBR_RS20675 and BBR_RS20680 overlap by 16 nucleotides
BBR_RS12220: BBR_RS12220 - isopeptide-forming domain-containing fimbrial protein, at 491,317 to 492,864
_RS12220
BBR_RS12225: BBR_RS12225 - hypothetical protein, at 492,973 to 493,647
_RS12225
BBR_RS20675: BBR_RS20675 - hypothetical protein, at 493,926 to 494,420
_RS20675
BBR_RS20680: BBR_RS20680 - hypothetical protein, at 494,405 to 494,641
_RS20680
Position (kb) 492
493
494 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 491.998 kb on + strand, within BBR_RS12220 at 492.035 kb on - strand, within BBR_RS12220 at 492.035 kb on - strand, within BBR_RS12220 at 492.037 kb on + strand, within BBR_RS12220 at 492.037 kb on + strand, within BBR_RS12220 at 492.043 kb on - strand, within BBR_RS12220 at 492.045 kb on - strand, within BBR_RS12220 at 492.047 kb on - strand, within BBR_RS12220 at 492.049 kb on + strand, within BBR_RS12220 at 492.054 kb on + strand, within BBR_RS12220 at 492.105 kb on - strand, within BBR_RS12220 at 492.105 kb on - strand, within BBR_RS12220 at 492.117 kb on - strand, within BBR_RS12220 at 492.119 kb on + strand, within BBR_RS12220 at 492.119 kb on + strand, within BBR_RS12220 at 492.119 kb on + strand, within BBR_RS12220 at 492.121 kb on - strand, within BBR_RS12220 at 492.127 kb on - strand, within BBR_RS12220 at 492.127 kb on - strand, within BBR_RS12220 at 492.127 kb on - strand, within BBR_RS12220 at 492.127 kb on - strand, within BBR_RS12220 at 492.127 kb on - strand, within BBR_RS12220 at 492.134 kb on - strand, within BBR_RS12220 at 492.134 kb on - strand, within BBR_RS12220 at 492.143 kb on + strand, within BBR_RS12220 at 492.156 kb on + strand, within BBR_RS12220 at 492.156 kb on + strand, within BBR_RS12220 at 492.156 kb on + strand, within BBR_RS12220 at 492.164 kb on - strand, within BBR_RS12220 at 492.164 kb on - strand, within BBR_RS12220 at 492.164 kb on - strand, within BBR_RS12220 at 492.164 kb on - strand, within BBR_RS12220 at 492.168 kb on + strand, within BBR_RS12220 at 492.168 kb on + strand, within BBR_RS12220 at 492.175 kb on + strand, within BBR_RS12220 at 492.209 kb on + strand, within BBR_RS12220 at 492.217 kb on - strand, within BBR_RS12220 at 492.220 kb on + strand, within BBR_RS12220 at 492.232 kb on + strand, within BBR_RS12220 at 492.237 kb on + strand, within BBR_RS12220 at 492.245 kb on - strand, within BBR_RS12220 at 492.260 kb on - strand, within BBR_RS12220 at 492.267 kb on - strand, within BBR_RS12220 at 492.286 kb on + strand, within BBR_RS12220 at 492.311 kb on - strand, within BBR_RS12220 at 492.319 kb on + strand, within BBR_RS12220 at 492.344 kb on + strand, within BBR_RS12220 at 492.352 kb on - strand, within BBR_RS12220 at 492.352 kb on - strand, within BBR_RS12220 at 492.352 kb on - strand, within BBR_RS12220 at 492.362 kb on - strand, within BBR_RS12220 at 492.363 kb on - strand, within BBR_RS12220 at 492.369 kb on + strand, within BBR_RS12220 at 492.379 kb on + strand, within BBR_RS12220 at 492.381 kb on + strand, within BBR_RS12220 at 492.387 kb on - strand, within BBR_RS12220 at 492.389 kb on + strand, within BBR_RS12220 at 492.389 kb on - strand, within BBR_RS12220 at 492.389 kb on - strand, within BBR_RS12220 at 492.396 kb on - strand, within BBR_RS12220 at 492.396 kb on - strand, within BBR_RS12220 at 492.408 kb on + strand, within BBR_RS12220 at 492.431 kb on - strand, within BBR_RS12220 at 492.438 kb on - strand, within BBR_RS12220 at 492.438 kb on - strand, within BBR_RS12220 at 492.438 kb on - strand, within BBR_RS12220 at 492.438 kb on - strand, within BBR_RS12220 at 492.445 kb on + strand, within BBR_RS12220 at 492.463 kb on + strand, within BBR_RS12220 at 492.473 kb on + strand, within BBR_RS12220 at 492.476 kb on - strand, within BBR_RS12220 at 492.479 kb on - strand, within BBR_RS12220 at 492.481 kb on - strand, within BBR_RS12220 at 492.520 kb on - strand, within BBR_RS12220 at 492.526 kb on + strand, within BBR_RS12220 at 492.534 kb on - strand, within BBR_RS12220 at 492.534 kb on - strand, within BBR_RS12220 at 492.534 kb on - strand, within BBR_RS12220 at 492.547 kb on + strand, within BBR_RS12220 at 492.557 kb on - strand, within BBR_RS12220 at 492.572 kb on - strand, within BBR_RS12220 at 492.586 kb on + strand, within BBR_RS12220 at 492.625 kb on - strand, within BBR_RS12220 at 492.639 kb on + strand, within BBR_RS12220 at 492.645 kb on + strand, within BBR_RS12220 at 492.654 kb on + strand, within BBR_RS12220 at 492.654 kb on + strand, within BBR_RS12220 at 492.659 kb on + strand, within BBR_RS12220 at 492.661 kb on - strand, within BBR_RS12220 at 492.682 kb on + strand, within BBR_RS12220 at 492.682 kb on + strand, within BBR_RS12220 at 492.688 kb on + strand, within BBR_RS12220 at 492.691 kb on + strand, within BBR_RS12220 at 492.713 kb on - strand at 492.718 kb on + strand at 492.752 kb on - strand at 492.772 kb on + strand at 492.772 kb on + strand at 492.777 kb on + strand at 492.785 kb on - strand at 492.806 kb on + strand at 492.904 kb on - strand at 492.912 kb on - strand at 492.940 kb on - strand at 493.011 kb on + strand at 493.038 kb on - strand at 493.043 kb on - strand, within BBR_RS12225 at 493.089 kb on - strand, within BBR_RS12225 at 493.089 kb on - strand, within BBR_RS12225 at 493.107 kb on + strand, within BBR_RS12225 at 493.110 kb on - strand, within BBR_RS12225 at 493.114 kb on + strand, within BBR_RS12225 at 493.117 kb on - strand, within BBR_RS12225 at 493.121 kb on + strand, within BBR_RS12225 at 493.175 kb on - strand, within BBR_RS12225 at 493.175 kb on - strand, within BBR_RS12225 at 493.175 kb on - strand, within BBR_RS12225 at 493.183 kb on + strand, within BBR_RS12225 at 493.183 kb on + strand, within BBR_RS12225 at 493.183 kb on - strand, within BBR_RS12225 at 493.191 kb on - strand, within BBR_RS12225 at 493.191 kb on - strand, within BBR_RS12225 at 493.216 kb on - strand, within BBR_RS12225 at 493.242 kb on + strand, within BBR_RS12225 at 493.246 kb on - strand, within BBR_RS12225 at 493.246 kb on - strand, within BBR_RS12225 at 493.261 kb on + strand, within BBR_RS12225 at 493.269 kb on - strand, within BBR_RS12225 at 493.293 kb on - strand, within BBR_RS12225 at 493.367 kb on - strand, within BBR_RS12225 at 493.369 kb on - strand, within BBR_RS12225 at 493.398 kb on - strand, within BBR_RS12225 at 493.510 kb on + strand, within BBR_RS12225 at 493.511 kb on - strand, within BBR_RS12225 at 493.511 kb on - strand, within BBR_RS12225 at 493.511 kb on - strand, within BBR_RS12225 at 493.555 kb on + strand, within BBR_RS12225 at 493.557 kb on - strand, within BBR_RS12225 at 493.598 kb on + strand at 493.599 kb on - strand at 493.604 kb on - strand at 493.606 kb on - strand at 493.612 kb on + strand at 493.650 kb on - strand at 493.650 kb on - strand at 493.674 kb on - strand at 493.692 kb on + strand at 493.700 kb on - strand at 493.700 kb on - strand at 493.706 kb on + strand at 493.706 kb on + strand at 493.742 kb on + strand at 493.742 kb on + strand at 493.763 kb on + strand at 493.771 kb on - strand at 493.773 kb on + strand at 493.773 kb on + strand at 493.814 kb on + strand at 493.820 kb on - strand at 493.825 kb on - strand at 493.825 kb on - strand at 493.850 kb on + strand at 493.876 kb on + strand at 493.884 kb on - strand at 493.884 kb on - strand at 493.901 kb on + strand at 493.910 kb on + strand at 493.910 kb on + strand at 493.910 kb on + strand at 493.911 kb on - strand at 493.918 kb on - strand at 493.920 kb on + strand at 493.920 kb on - strand at 493.959 kb on - strand at 493.959 kb on - strand at 493.992 kb on + strand, within BBR_RS20675 at 494.001 kb on + strand, within BBR_RS20675 at 494.001 kb on + strand, within BBR_RS20675 at 494.021 kb on - strand, within BBR_RS20675 at 494.037 kb on - strand, within BBR_RS20675 at 494.114 kb on + strand, within BBR_RS20675 at 494.127 kb on - strand, within BBR_RS20675 at 494.129 kb on + strand, within BBR_RS20675 at 494.163 kb on - strand, within BBR_RS20675 at 494.163 kb on - strand, within BBR_RS20675 at 494.224 kb on - strand, within BBR_RS20675 at 494.248 kb on + strand, within BBR_RS20675 at 494.266 kb on + strand, within BBR_RS20675 at 494.390 kb on + strand at 494.390 kb on + strand at 494.390 kb on + strand at 494.390 kb on + strand at 494.395 kb on + strand at 494.395 kb on + strand at 494.423 kb on + strand at 494.440 kb on - strand, within BBR_RS20680 at 494.440 kb on - strand, within BBR_RS20680 at 494.470 kb on + strand, within BBR_RS20680 at 494.470 kb on + strand, within BBR_RS20680 at 494.470 kb on + strand, within BBR_RS20680 at 494.478 kb on - strand, within BBR_RS20680 at 494.482 kb on + strand, within BBR_RS20680 at 494.491 kb on - strand, within BBR_RS20680 at 494.588 kb on + strand, within BBR_RS20680 at 494.604 kb on + strand, within BBR_RS20680 at 494.618 kb on - strand at 494.626 kb on + strand at 494.626 kb on + strand at 494.628 kb on - strand at 494.634 kb on - strand at 494.636 kb on - strand at 494.636 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Cysteine remove 491,998 + BBR_RS12220 0.44 -0.9 492,035 - BBR_RS12220 0.46 -2.0 492,035 - BBR_RS12220 0.46 +0.2 492,037 + BBR_RS12220 0.47 +1.7 492,037 + BBR_RS12220 0.47 -0.3 492,043 - BBR_RS12220 0.47 -0.0 492,045 - BBR_RS12220 0.47 +0.3 492,047 - BBR_RS12220 0.47 -0.7 492,049 + BBR_RS12220 0.47 -1.4 492,054 + BBR_RS12220 0.48 -0.9 492,105 - BBR_RS12220 0.51 -1.0 492,105 - BBR_RS12220 0.51 +2.2 492,117 - BBR_RS12220 0.52 -0.3 492,119 + BBR_RS12220 0.52 +0.7 492,119 + BBR_RS12220 0.52 +2.2 492,119 + BBR_RS12220 0.52 +0.2 492,121 - BBR_RS12220 0.52 -0.7 492,127 - BBR_RS12220 0.52 -0.3 492,127 - BBR_RS12220 0.52 +0.2 492,127 - BBR_RS12220 0.52 -0.1 492,127 - BBR_RS12220 0.52 -0.9 492,127 - BBR_RS12220 0.52 +0.2 492,134 - BBR_RS12220 0.53 +0.1 492,134 - BBR_RS12220 0.53 -0.5 492,143 + BBR_RS12220 0.53 -1.8 492,156 + BBR_RS12220 0.54 -0.2 492,156 + BBR_RS12220 0.54 +0.7 492,156 + BBR_RS12220 0.54 -0.1 492,164 - BBR_RS12220 0.55 -0.2 492,164 - BBR_RS12220 0.55 -0.2 492,164 - BBR_RS12220 0.55 +0.1 492,164 - BBR_RS12220 0.55 -0.6 492,168 + BBR_RS12220 0.55 +0.5 492,168 + BBR_RS12220 0.55 +0.2 492,175 + BBR_RS12220 0.55 -0.5 492,209 + BBR_RS12220 0.58 -0.3 492,217 - BBR_RS12220 0.58 +0.5 492,220 + BBR_RS12220 0.58 +0.3 492,232 + BBR_RS12220 0.59 +0.4 492,237 + BBR_RS12220 0.59 -0.0 492,245 - BBR_RS12220 0.60 +1.2 492,260 - BBR_RS12220 0.61 -0.2 492,267 - BBR_RS12220 0.61 -0.7 492,286 + BBR_RS12220 0.63 -2.2 492,311 - BBR_RS12220 0.64 +0.3 492,319 + BBR_RS12220 0.65 +0.5 492,344 + BBR_RS12220 0.66 +0.2 492,352 - BBR_RS12220 0.67 -1.6 492,352 - BBR_RS12220 0.67 +0.9 492,352 - BBR_RS12220 0.67 +1.2 492,362 - BBR_RS12220 0.68 -0.1 492,363 - BBR_RS12220 0.68 +0.1 492,369 + BBR_RS12220 0.68 +1.3 492,379 + BBR_RS12220 0.69 +0.9 492,381 + BBR_RS12220 0.69 -0.2 492,387 - BBR_RS12220 0.69 -0.7 492,389 + BBR_RS12220 0.69 -0.2 492,389 - BBR_RS12220 0.69 +2.4 492,389 - BBR_RS12220 0.69 +0.2 492,396 - BBR_RS12220 0.70 -0.2 492,396 - BBR_RS12220 0.70 +0.1 492,408 + BBR_RS12220 0.70 +1.5 492,431 - BBR_RS12220 0.72 -0.4 492,438 - BBR_RS12220 0.72 -0.2 492,438 - BBR_RS12220 0.72 +0.4 492,438 - BBR_RS12220 0.72 -0.6 492,438 - BBR_RS12220 0.72 +1.0 492,445 + BBR_RS12220 0.73 +0.4 492,463 + BBR_RS12220 0.74 +1.9 492,473 + BBR_RS12220 0.75 +0.1 492,476 - BBR_RS12220 0.75 -0.1 492,479 - BBR_RS12220 0.75 -0.9 492,481 - BBR_RS12220 0.75 -0.3 492,520 - BBR_RS12220 0.78 +0.2 492,526 + BBR_RS12220 0.78 +0.4 492,534 - BBR_RS12220 0.79 +0.4 492,534 - BBR_RS12220 0.79 -0.3 492,534 - BBR_RS12220 0.79 +0.2 492,547 + BBR_RS12220 0.79 +0.4 492,557 - BBR_RS12220 0.80 -0.4 492,572 - BBR_RS12220 0.81 -0.7 492,586 + BBR_RS12220 0.82 +1.7 492,625 - BBR_RS12220 0.84 +0.7 492,639 + BBR_RS12220 0.85 -0.0 492,645 + BBR_RS12220 0.86 -0.9 492,654 + BBR_RS12220 0.86 -1.0 492,654 + BBR_RS12220 0.86 +0.8 492,659 + BBR_RS12220 0.87 +0.6 492,661 - BBR_RS12220 0.87 -0.6 492,682 + BBR_RS12220 0.88 +0.1 492,682 + BBR_RS12220 0.88 +0.6 492,688 + BBR_RS12220 0.89 -0.5 492,691 + BBR_RS12220 0.89 -1.6 492,713 - +0.3 492,718 + -1.1 492,752 - +0.7 492,772 + -0.6 492,772 + -1.8 492,777 + -0.3 492,785 - +0.1 492,806 + +0.5 492,904 - +0.3 492,912 - +0.5 492,940 - -0.6 493,011 + +0.3 493,038 - +1.2 493,043 - BBR_RS12225 0.10 -0.4 493,089 - BBR_RS12225 0.17 -1.4 493,089 - BBR_RS12225 0.17 -0.7 493,107 + BBR_RS12225 0.20 -0.3 493,110 - BBR_RS12225 0.20 +0.0 493,114 + BBR_RS12225 0.21 -0.9 493,117 - BBR_RS12225 0.21 -1.2 493,121 + BBR_RS12225 0.22 -1.4 493,175 - BBR_RS12225 0.30 -1.7 493,175 - BBR_RS12225 0.30 -1.3 493,175 - BBR_RS12225 0.30 -0.6 493,183 + BBR_RS12225 0.31 -1.1 493,183 + BBR_RS12225 0.31 +1.4 493,183 - BBR_RS12225 0.31 -0.4 493,191 - BBR_RS12225 0.32 +0.1 493,191 - BBR_RS12225 0.32 -0.2 493,216 - BBR_RS12225 0.36 -0.0 493,242 + BBR_RS12225 0.40 -0.5 493,246 - BBR_RS12225 0.40 +0.2 493,246 - BBR_RS12225 0.40 -3.2 493,261 + BBR_RS12225 0.43 -1.9 493,269 - BBR_RS12225 0.44 -1.5 493,293 - BBR_RS12225 0.47 -1.3 493,367 - BBR_RS12225 0.58 -0.9 493,369 - BBR_RS12225 0.59 -1.4 493,398 - BBR_RS12225 0.63 +1.4 493,510 + BBR_RS12225 0.80 +0.1 493,511 - BBR_RS12225 0.80 -1.3 493,511 - BBR_RS12225 0.80 -0.1 493,511 - BBR_RS12225 0.80 -2.5 493,555 + BBR_RS12225 0.86 -1.2 493,557 - BBR_RS12225 0.87 -1.9 493,598 + -1.6 493,599 - +0.1 493,604 - -0.7 493,606 - -0.3 493,612 + -2.5 493,650 - -0.0 493,650 - -0.0 493,674 - +0.2 493,692 + -0.3 493,700 - -0.4 493,700 - -0.5 493,706 + +0.1 493,706 + +0.1 493,742 + -2.6 493,742 + -1.3 493,763 + -0.9 493,771 - +0.4 493,773 + -0.7 493,773 + -0.4 493,814 + +0.1 493,820 - +1.0 493,825 - +0.0 493,825 - -0.4 493,850 + -0.8 493,876 + -1.8 493,884 - -0.5 493,884 - +1.3 493,901 + -0.1 493,910 + +0.3 493,910 + -0.1 493,910 + -0.2 493,911 - +1.5 493,918 - +0.6 493,920 + -0.2 493,920 - -0.3 493,959 - -0.1 493,959 - +0.8 493,992 + BBR_RS20675 0.13 -0.4 494,001 + BBR_RS20675 0.15 +0.3 494,001 + BBR_RS20675 0.15 +0.7 494,021 - BBR_RS20675 0.19 -1.4 494,037 - BBR_RS20675 0.22 -0.2 494,114 + BBR_RS20675 0.38 -0.9 494,127 - BBR_RS20675 0.41 +1.2 494,129 + BBR_RS20675 0.41 -0.9 494,163 - BBR_RS20675 0.48 +0.7 494,163 - BBR_RS20675 0.48 +0.8 494,224 - BBR_RS20675 0.60 +0.1 494,248 + BBR_RS20675 0.65 -0.4 494,266 + BBR_RS20675 0.69 -2.4 494,390 + -0.5 494,390 + +0.0 494,390 + +1.1 494,390 + -2.4 494,395 + +0.3 494,395 + -0.5 494,423 + -1.0 494,440 - BBR_RS20680 0.15 +0.1 494,440 - BBR_RS20680 0.15 -1.3 494,470 + BBR_RS20680 0.27 -0.5 494,470 + BBR_RS20680 0.27 -1.5 494,470 + BBR_RS20680 0.27 -0.9 494,478 - BBR_RS20680 0.31 +0.0 494,482 + BBR_RS20680 0.32 -0.7 494,491 - BBR_RS20680 0.36 -1.1 494,588 + BBR_RS20680 0.77 -0.5 494,604 + BBR_RS20680 0.84 -0.3 494,618 - -0.1 494,626 + +0.5 494,626 + -0.9 494,628 - +0.7 494,634 - +0.9 494,636 - -0.4 494,636 - -0.6
Or see this region's nucleotide sequence